BF0993 (B. fragilis NCTC 9343 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF0993
Gene Name:  hypothetical protein
Original Clone ID:
APC62121_SG173_pMCSG19
Keyword:
full length
Species:
Bacteroides fragilis NCTC 9343
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
BfCD00342381
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1254nts
Open reading frame: 1 to 1254

ATGAGCATCATTATTTACCTGTTGATTACGATGGCATTTTCTGCTTTCTTTTCGGGAATG
GAGATTGCTTTTGTTTCGGTAGACAAACTTCGTTTTGAAATGGACCGGAAAGGAGGGGTA
TCATCGCGTATCCTTTCGCTATTCTTCCGGAATCCCAATGATTTTATTTCGACCATGCTG
GTCGGGAATAACATCGCTCTGGTTATCTATGGTATATTGATGGCACAGATTATCGGCGAC
AATTTGCTGGCCGGATGGATCACCAATCATTTTGTAATGGTATTGGTACAGACCGTGATC
TCCACACTGATCATCTTGGTGACAGGAGAGTTTCTGCCCAAAACGCTTTTTAAGATCAAT
CCCAATCTGGCCCTGAATGTCTGTGCAGTTCCTCTTTTCATCTGTTATGTTGTTCTCTAT
CCTATATCTAAATTTTCGTCGGGAGTCTCTTACCTCTTTCTTCGCCTGTTTGGGATGAAA
GTGAACAAGGAAGCCTCTGCGAAAGCCTTTGGTAAGGTAGATCTGGATTATTTTGTCCAG
TCGAGTATAGACAATGCCGAAAGTGAGGAAACTCTGGACACGGAAGTGAAAATCTTTCAG
AATGCACTCGACTTCTCGGCGGTCAAGATACGCGACTGCATCGTTCCACGGACAGAAGTG
GTAGCTGTTGCGCTGGATACATCCCTTGAAGAACTTAAAGGCCGGTTTGTTGAGTCCGGT
ATATCAAAAATAATTGTCTACGATGGTAATATAGATAATGTGGTGGGATACATTCATTCG
TCCGAAATGTTTCGTAGCCCGAAAGATTGGCGCAATCATGTGAAAGAAGTTCCCATTGTG
CCTGAAACGATGGCGGCCCATAAACTGATGAAGCTGTTTATGCAACAGAAAAAGACCATT
GCCGTGGTAGTGGATGAATTTGGAGGAACTTCGGGTATTGTCAGCCTTGAAGACCTTGTT
GAAGAAATTTTCGGTGACATCGAAGACGAACACGACAACACTTCCTATATTTGTAAGCAG
ATCGGTGAGCATGAATACGTGCTTTCAGCCCGTTTGGAAATAGAAAAAGTAAACGAAACT
TTTAATCTGGAGCTTCCCGAATCCGATGACTATCTAACGGTGGGAGGATTAATCTTGAAT
CAATACCAGAGCTTTCCGAAATTGCACGAATTGGTCTCTGTCGGTAAATATCAGTTTAAG
ATAATTAAGGTTACAGCAACGAAAATCGAACTTGTCCGACTGAAAGTAATGGAA

Protein sequence predicted based on nucleotide sequence

MSIIIYLLITMAFSAFFSGMEIAFVSVDKLRFEMDRKGGVSSRILSLFFRNPNDFISTML
VGNNIALVIYGILMAQIIGDNLLAGWITNHFVMVLVQTVISTLIILVTGEFLPKTLFKIN
PNLALNVCAVPLFICYVVLYPISKFSSGVSYLFLRLFGMKVNKEASAKAFGKVDLDYFVQ
SSIDNAESEETLDTEVKIFQNALDFSAVKIRDCIVPRTEVVAVALDTSLEELKGRFVESG
ISKIIVYDGNIDNVVGYIHSSEMFRSPKDWRNHVKEVPIVPETMAAHKLMKLFMQQKKTI
AVVVDEFGGTSGIVSLEDLVEEIFGDIEDEHDNTSYICKQIGEHEYVLSARLEIEKVNET
FNLELPESDDYLTVGGLILNQYQSFPKLHELVSVGKYQFKIIKVTATKIELVRLKVME

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
ProteinExpressed: Tested_Not_Found
Find experimental protocols in APC62121
Recommended expression in:
Not Applicable