SPy_1110 (Streptococcus pyogenes) in pSGX4

Explanation of Terms

Gene:
Gene Symbol:  SPy_1110
Gene Name:  NAD-dependent malic enzyme
Original Clone ID:
6108d2BSt10b1
Keyword:
None
Species:
Streptococcus pyogenes
Type:
cDNA
Vector Name:
pSGX4
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SpCD00342421
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1170nts
Open reading frame: 1 to 1170

AAAAATCAATTAGGTCAACTTGCCCTTGAACAAGCCAAAACGTTCGGCGGTAAATTGGAA
GTTCAACCAAAAGTTGACATTAAAACTAAACATGATTTGAGTATTGCCTACACACCAGGT
GTAGCGAGTGTGTCATCTGCCATCGCAAAAGATAAAACATTAGCATATGACTTAACCACC
AAGAAAAATACAGTAGCCGTTATCAGTGATGGTACTGCGGTTCTTGGTCTGGGGGATATT
GGCCCTGAAGCAGCCATGCCAGTTATGGAAGGAAAAGCAGCCCTTTTTAAAGCATTTGCG
GGTGTTGATGCTATTCCGATTGTATTAGATACCAAAGATACTGAAGAAATTATTAGTATT
GTCAAGGCGCTTGCACCAACCTTTGGTGGTATTAATTTAGAGGATATTAGTGCGCCCCGC
TGTTTTGAAATTGAGCAGCGCTTGATTAAGGAATGTCATATTCCTGTTTTCCATGACGAT
CAGCATGGAACAGCTATTGTGGTGTTAGCAGCCATCTTCAATAGTTTGAAACTTCTTAAA
AAATCCCTTGATGAGGTATCCATTGTGGTGAATGGTGGTGGATCTGCTGGATTATCCATT
ACCCGTAAATTATTAGCAGCAGGTGCTACGAAAGTGACAGTAGTGGATAAATTTGGCATT
ATCAATGAACAAGAAGCCGCTCAATTAGCGCCACATCATTTGGATATAGCGAAAGTCACT
AATCGTGAGTTTAAATCAGGTACCTTAGAAGATGCTCTTGAAGGAGCAGATATCTTCATT
GGCGTATCAGCACCAGGAGTCTTAAAAGCAGAGTGGATTAGTAAAATGGCAGCGCGCCCA
GTAATTTTTGCCATGGCCAACCCTATTCCAGAGATTTACCCCGATGAAGCCCTTGAAGCG
GGGGCTTACATTGTAGGTACTGGCCGCAGTGATTTTCCTAACCAGATTAATAATGTCCTT
GCCTTTCCAGGTATTTTTAGGGGAGCATTAGATGCACGGGCAAAAACCATTACTGTTGAG
ATGCAAATTGCTGCCGCAAAAGGTATTGCCAGTCTTGTCCCAGATGACGCTTTGAGCACT
ACTAATATTATTCCAGATGCCTTTAAAGAAGGAGTCGCTGAGATTGTCGCTAAAAGCGTC
AGGTCGGTAGTCCTTAAATCAGAAGGGTAA

Protein sequence predicted based on nucleotide sequence

KNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVASVSSAIAKDKTLAYDLTT
KKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAGVDAIPIVLDTKDTEEIISI
VKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHDDQHGTAIVVLAAIFNSLKLLK
KSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDKFGIINEQEAAQLAPHHLDIAKVT
NREFKSGTLEDALEGADIFIGVSAPGVLKAEWISKMAARPVIFAMANPIPEIYPDEALEA
GAYIVGTGRSDFPNQINNVLAFPGIFRGALDARAKTITVEMQIAAAKGIASLVPDDALST
TNIIPDAFKEGVAEIVAKSVRSVVLKSEG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t882c,P294P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6108d
Recommended expression in:
Not Applicable