RPA3550 (R. palustris CGA009 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  RPA3550
Gene Name:  coenzyme B12-binding elongator protein 3/MiaB/NifB
Original Clone ID:
APC39179.2_SG264_pMCSG19B
Keyword:
partial cds
Species:
Rhodopseudomonas palustris CGA009
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
RpCD00343063
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1206nts
Open reading frame: 1 to 1206

GGAGACGGCTTCGACTACGGCCTTTGGCGCGATCTGGTGCGCGATGCGATCAAGGCCTGC
GCCACCCCGCATGTCGGCGTGTCGTACATGTTCGGCACCACCAAGCCGTGCTTCACCGAA
GTGATCGACCTGATCCGCAGCGAATTCCCGCAGGTGCCGGTCCTGTGCGGCGGCGTGCAG
GCGACGTTCGACTATCGGGAGATCCTGGCGAGCGGGCTGTGCGACATCGTCGCCCGCCAC
GAAGGCGAACAGCAACTGATCGGCTATCTCGGCGCGCTGGCCGGCAATCCGGATGCCGTT
CCGGTCGGCATGGCGTTCACCGAGGCCGGCGCTATCGTCGAACTCGGCGCACCGCCACCG
GATAATCCGGTGAACTGGGACGTCACACCGTTCTACGACGCGATCCGGATCGAGGAGTAT
CACCGCTACGGCGGGCTGGGCGCGTTCTCGCGCTATGTCGGCGCCGACAAGCCGTTCGCC
ACGGTGCTCGCCGCGCGCGGCTGCCGCGCCCGCTGCACTTTCTGCACGGTGCGCAACTTC
AACGGCTTCGGCCTGCGGCAGCGCACGGTGCAGGACGTCATCGACGAGGTGAAGCACCTC
GTGACCGATCACGGCATCCGCTCGATCGACTGGCTCGACGACGATCTGCTGTGGGACCGG
GAGCGCGCGCTGGCGCTGTTCAGGGGCCTCGCCGAGCAAGTGCCGGGGCTGGAATGGACC
GCCAGCAACGGCCTGATCGCGGTGGCGATCGACGACGAGGTGATGCAGGCGATGGTCGCG
TCCGGCCTGCGCGCCTTCAAGATCGGTGTCGAGTCCGGCAACGACGCGATGCTGAAGACG
ATCAAGAAGCCGACCACCAAGCCGAAGCTGCGCGCCCGCGCCGAGCTGTTCGCCAGATAT
CCGCAAGTGTTCGTCTCGACCAATTTCATCATCGGCTTTCCCGACGAAACCTTCGGGCAG
ATGATGGACAGCTACGATCTGGCCGTCGAGCTCGCCAGCGACTGGGCCAGCTTCTACATC
TGCCAGCCGCTGAAGGGCACCGAGATGTTCTCGGCGTTCCAATCGCTCGGCGATGCCCGC
ACCAAGGAAGAGCGCTACGACAAGACCATCAATCCCGGACGCTCCGCCGAGCGCGGCGAA
TTCGGCTACCGCTTCGCGCCCGACCGCGTGCTGCGCACCGGCTGGGAGGTGTTCGAGATA
GATTAA

Protein sequence predicted based on nucleotide sequence

GDGFDYGLWRDLVRDAIKACATPHVGVSYMFGTTKPCFTEVIDLIRSEFPQVPVLCGGVQ
ATFDYREILASGLCDIVARHEGEQQLIGYLGALAGNPDAVPVGMAFTEAGAIVELGAPPP
DNPVNWDVTPFYDAIRIEEYHRYGGLGAFSRYVGADKPFATVLAARGCRARCTFCTVRNF
NGFGLRQRTVQDVIDEVKHLVTDHGIRSIDWLDDDLLWDRERALALFRGLAEQVPGLEWT
ASNGLIAVAIDDEVMQAMVASGLRAFKIGVESGNDAMLKTIKKPTTKPKLRARAELFARY
PQVFVSTNFIIGFPDETFGQMMDSYDLAVELASDWASFYICQPLKGTEMFSAFQSLGDAR
TKEERYDKTINPGRSAERGEFGYRFAPDRVLRTGWEVFEID*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC39179.2
Recommended expression in:
Not Applicable