Gene:
Original Clone ID:
NCI10295
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
HsCD00343140
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG
ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT
TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA
CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT
TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG
GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG
GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGATTCCCATCACCTATCCG
CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC
ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT
CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAA
Protein sequence predicted based on nucleotide sequence
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPP PVKKRKRKCLLL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Confirmed
SolubleProtein: Protein_Confirmed
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
Gene
Gene Symbol :
RAC1
Symbol Nomenclature :
RAC1
SYNONYM :
Rac-1; TC-25; p21-Rac1
Designation :
cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1
Full Nomenclature :
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GENEID :
Locus Tag :
MIG5
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Reference Sequence Alignment
HsCD00343140 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG NM_005052.2 ATGCAGGCCATCAAGTGCGTGGTGGTCGGCGACGGCGCCGTGGGGAAGACATGCTTGCTG NM_006908.4 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG NM_002872.4 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGATGGGGCCGTGGGCAAGACCTGCCTTCTC ***************** ******** **.** ** ** **.** **.** *** * ** HsCD00343140 ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT NM_005052.2 ATCAGCTACACGACCAACGCCTTCCCCGGAGAGTACATCCCCACCGTTTTTGACAACTAC NM_006908.4 ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT NM_002872.4 ATCAGCTACACCACCAACGCCTTTCCCGGAGAGTACATCCCCACCGTGTTTGACAACTAT ***** ***** ***** **.** ** *****.** ***** ** ** ******** ** HsCD00343140 TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA NM_005052.2 TCTGCCAACGTGATGGTGGACGGGAAACCAGTCAACTTGGGGCTGTGGGACACAGCGGGT NM_006908.4 TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA NM_002872.4 TCAGCCAATGTGATGGTGGACAGCAAGCCAGTGAACCTGGGGCTGTGGGACACTGCTGGG **:***** ** *****.** .* **.**.** ** **** *.***** **:** ** HsCD00343140 CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT NM_005052.2 CAGGAGGACTACGATCGGCTGCGGCCACTCTCCTACCCCCAAACTGACGTCTTTCTGATC NM_006908.4 CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT NM_002872.4 CAGGAGGACTACGACCGTCTCCGGCCGCTCTCCTATCCACAGACGGACGTCTTCCTCATC **.**.** ** ** .* * ** ** **.***** ** **.** ** ** ** * ** HsCD00343140 TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG NM_005052.2 TGCTTCTCTCTGGTGAGCCCGGCCTCCTTCGAGAATGTTCGTGCCAAGTGGTACCCGGAG NM_006908.4 TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG NM_002872.4 TGCTTCTCCCTCGTCAGCCCAGCCTCTTATGAGAACGTCCGCGCCAAGTGGTTCCCAGAA ***** ** ** ** ** ** **.** *: **.** ** ** **.*******: ** **. HsCD00343140 GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG NM_005052.2 GTGCGGCACCACTGCCCCCACACGCCCATCCTCCTGGTGGGCACCAAGCTGGACCTCCGC NM_006908.4 GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG NM_002872.4 GTGCGGCACCACTGCCCCAGCACACCCATCATCCTGGTGGGCACCAAGCTGGACCTGCGG ************** ***..*** ******.****.*****.** **.** ** ** .* HsCD00343140 GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGATTCCCATCACCTATCCG NM_005052.2 GACGACAAGGACACCATTGAGCGGCTGCGGGACAAGAAGCTGGCACCCATCACCTACCCA NM_006908.4 GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGACTCCCATCACCTATCCG NM_002872.4 GACGACAAGGACACCATCGAGAAACTGAAGGAGAAGAAGCTGGCTCCCATCACCTACCCG ** ** **.***** ** ***...***..*** *********. :*********** **. HsCD00343140 CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC NM_005052.2 CAGGGCCTGGCCATGGCCCGGGAGATTGGCTCTGTGAAATACCTGGAGTGCTCAGCCCTG NM_006908.4 CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC NM_002872.4 CAGGGCCTGGCACTGGCCAAGGAGATTGACTCGGTGAAATACCTGGAGTGCTCAGCTCTC ***** **.**..**** ..********. * **.*****************.** ** HsCD00343140 ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT NM_005052.2 ACCCAGCGGGGCCTGAAGACAGTGTTTGACGAGGCGATCCGCGCGGTGCTCTGCCCGCCC NM_006908.4 ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT NM_002872.4 ACCCAGAGAGGCCTGAAAACCGTGTTCGACGAGGCCATCCGGGCCGTGCTGTGCCCTCAG **.***.*.***** **.**.***** *****.** ***** ** ** ** ***** *. HsCD00343140 CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAA NM_005052.2 CCAGTGAAGAAGCCGGGGAAGAAGTGCACCGTCTTCTAG NM_006908.4 CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAA NM_002872.4 CCCACGCGGCAGCAGAAGCGCGCCTGCAGCCTCCTCTAG **.. *..*.**. *..*.. .. ***. * * **.
Pathways
NCI :
Alpha4 beta1 integrin signaling events
NCI :
Alpha9 beta1 integrin signaling events
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Arf1 pathway
NCI :
Arf6 downstream pathway
NCI :
BCR signaling pathway
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR4-mediated signaling events
NCI :
E-cadherin signaling in keratinocytes
NCI :
E-cadherin signaling in the nascent adherens junction
NCI :
Endothelins
NCI :
EPHA2 forward signaling
NCI :
EPHB forward signaling
NCI :
Ephrin B reverse signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL6-mediated signaling events
NCI :
Integrin-linked kinase signaling
NCI :
Integrins in angiogenesis
NCI :
Lissencephaly gene (LIS1) in neuronal migration and development
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
N-cadherin signaling events
NCI :
Nectin adhesion pathway
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Noncanonical Wnt signaling pathway
NCI :
Nongenotropic Androgen signaling
NCI :
Osteopontin-mediated events
NCI :
p38 MAPK signaling pathway
NCI :
p75(NTR)-mediated signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
RAC1 signaling pathway
NCI :
Regulation of p38-alpha and p38-beta
NCI :
Regulation of RAC1 activity
NCI :
S1P1 pathway
NCI :
S1P2 pathway
NCI :
S1P3 pathway
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PRL
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-4-mediated signaling events
NCI :
Thromboxane A2 receptor signaling
NCI :
Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling
Panther :
Axon guidance mediated by netrin
Panther :
Axon guidance mediated by semaphorins
Panther :
Axon guidance mediated by Slit/Robo
Panther :
B cell activation
Panther :
Cytoskeletal regulation by Rho GTPase
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Huntington disease
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Integrin signalling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
VEGF signaling pathway
Reactome :
Activation of Rac
Reactome :
Adaptive Immune System
Reactome :
Axon guidance
Reactome :
beta-catenin independent WNT signaling
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent Vav1 pathway
Reactome :
Cell death signalling via NRAGE, NRIF and NADE
Reactome :
Cell-Cell communication
Reactome :
Costimulation by the CD28 family
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
DCC mediated attractive signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
DSCAM interactions
Reactome :
Factors involved in megakaryocyte development and platelet production
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
G alpha (12/13) signalling events
Reactome :
GPCR downstream signaling
Reactome :
GPVI-mediated activation cascade
Reactome :
Hemostasis
Reactome :
HIV Infection
Reactome :
Host Interactions of HIV factors
Reactome :
Immune System
Reactome :
Inactivation of Cdc42 and Rac
Reactome :
Innate Immune System
Reactome :
L1CAM interactions
Reactome :
Membrane Trafficking
Reactome :
Nef and signal transduction
Reactome :
Netrin-1 signaling
Reactome :
NRAGE signals death through JNK
Reactome :
p75 NTR receptor-mediated signalling
Reactome :
PCP/CE pathway
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Sema3A PAK dependent Axon repulsion
Reactome :
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
Reactome :
Sema4D in semaphorin signaling
Reactome :
Sema4D induced cell migration and growth-cone collapse
Reactome :
Sema4D mediated inhibition of cell attachment and migration
Reactome :
Semaphorin interactions
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by GPCR
Reactome :
Signaling by Robo receptor
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
The role of Nef in HIV-1 replication and disease pathogenesis
Reactome :
Translocation of GLUT4 to the plasma membrane
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Focal Adhesion
WikiPathway :
G13 Signaling Pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
NOD pathway
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TOR signaling
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Wnt Signaling Pathway
Protein
SMART domain :
SMART domain :
SMART domain :
UniProt :
UniProt :
UniProt :
HPRD :
03627
Clone
Organism
Species Specific ID:
Genome
Reagents
Labome :