Gene:
Gene Symbol:
MAGI1
Gene Name:
membrane associated guanylate kinase, WW and PDZ domain containing 1
Original Clone ID:
NCI10356
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
HsCD00343154
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGTCCAAAGTGATCCAGAAGAAGAACCACTGGACTAGCAGGGTTCACGAATGCACCGTG
AAGCGGGGACCCCAGGGCGAGCTGGGGGTGACGGTGCTGGGAGGCGCGGAGCACGGGGAG
TTTCCGTACGTCGGAGCGGTGGCGGCGGTCGAGGCAGCGGGGCTTCCCGGCGGCGGCGAG
GGCCCGAGGCTGGGCGAAGGGGAGCTGCTTCTGGAGGTGCAGGGGGTCCGGGTGTCCGGC
TTGCCCCGCTATGACGTGCTGGGGGTCATCGACAGCTGCAAGGAGGCCGTCACCTTCAAG
GCCGTCAGACAAGGAGGAAGGCTGAACAAGGACCTGCGACATTTTCTCAATCAGCGATTC
CAGAAGGGGTCTCCTGATCATGAGCTCCAGCAGACCATAAGGGATAACCTTTACCGCCAT
GCTGTGCCTTGCACAACCCGATCTCCCAGAGAAGGAGAAGTGCCTGGCGTGGACTATAAC
TTTCTGACTGTGAAGGAGTTCTTGGACCTCGAGCAGAGTGGGACTCTTCTGGAAGTCGGC
ACCTATGAAGGAAACTATTATGGGACACCCAAGCCTCCTAGCCAGCCAGTCAGTGGGAAA
GTGATCACGACGGATGCCTTGCACAGCCTTCAGTCTGGCTCTAAGCAGTCGACCCCGAAG
CGAACCAAGTCCTACAATGATATGCAAAATGCTGGCATAGTCCACGCGGAGAATGAGGAG
GAGGATGACGTTCCTGAAATGAACAGCAGCTTTACAGCCGATTCTGGTGAACAAGAGGAG
CACACTCTCCAAGAAACAGCATTACCACCTGTGAATAGTAGCATCATCGCTGCTCCCATC
ACGGACCCTTCTCAGAAGTTCCCTCAATACCTACCTCTTTCTGCAGAGGATAATTTAGGT
CCTCTACCTGAAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTTATAGAC
CATAACACGAAAACAACATCTTGGTTAGACCCTCGGTGCCTAAACAAGCAGCAGAAGCCA
CTGGAAGAGTGTGAAGATGATGAAGGGGTACACACCGAGGAGCTGGACAGTGAACTAGAA
CTGCCTGCTGGTTGGGAAAAGATTGAAGACCCTGTCTATGGTATCTACTATGTAGACCAC
ATCAACAGGAAGACACAATATGAGAACCCGGTTCTAGAAGCCAAACGGAAGAAGCAGCTT
GAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGACAGAAGAATGGACAGAAGATCACTCAGCCCTTGTGCCTCCTGTTATTCCAAACCAC
CCTCCAAGCAATCCAGAGCCAGCCAGAGAAGTTCCACTTCAGGGCAAACCCTTTTTTACA
CGAAACCCTTCTGAGTTGAAAGGCAAGTTCATTCACACAAAGCTGCGGAAAAGCAGTCGT
GGCTTTGGCTTCACGGTGGTTGGAGGGGATGAACCTGATGAGTTTCTCCAGATCAAGAGC
TTGGTCCTAGATGGTCCTGCTGCATTGGATGGCAAGATGGAAACAGGGGATGTGATTGTA
AGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAAGATCTTCCAG
TCCATCCCCATTGGTGCCAGCGTGGACCTTGAACTCTGCCGAGGTTATCCATTGCCTTTT
GATCCAGATGACCCCAATACAAGTTTAGTGACCTCGGTAGCCATTTTGGATAAAGAACCA
ATTATTGTGAATGGGCAAGAGACCTATGATTCACCAGCTAGCCACAGTAGTAAAACAGGC
AAAGTCAATGGCATGAAGGATGCCAGGCCAAGCAGCCCAGCGGACGTGGCTTCAAATAGT
TCTCATGGTTATCCTAATGACACTGTTTCTTTGGCTTCCTCCATAGCCACTCAGCCAGAA
CTCATAACTGTTCATATTGTCAAAGGGCCAATGGGCTTTGGTTTTACTATCGCAGACAGT
CCTGGTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACAGCCCAAGGTGCCGAGGCCTG
AAAGAAGGGGATCTCATAGTGGAAGTTAATAAGAAGAACGTGCAGGCCCTAACTCACAAC
CAAGTGGTGGATATGCTGGTTGAGTGTCCTAAGGGAAGTGAGGTCACATTGTTGGTGCAA
CGAGGAGGGCTGCCAGTTCCCAAGAAGAGCCCAAAGTCGCAACCACTGGAAAGGAAAGAC
AGCCAGAATAGTTCTCAGCACAGTGTTTCCAGCCACCGAAGCCTGCACACAGCATCCCCA
AGCCACAGCACACAGGTGCTCCCCGAGTTCCCACCTGCAGAGGCCCAAGCTCCAGATCAA
ACTGACAGCTCTGGCCAGAAAAAACCAGATCCTTTTAAAATCTGGGCCCAGTCCAGGAGC
ATGTATGAAAACCGACCTATGTCACCTTCACCTGCGTCGGGATTGAGCAAGGGTGAGAGA
GAGAGAGAAATCAATTCCACGAATTTTGGAGAATGTCCGATTCCAGATTACCAGGAACAG
GACATCTTCCTCTGGAGAAAAGAGACTGGATTTGGATTTAGGATTCTGGGTGGAAATGAA
CCAGGGGAACCTATTTATATTGGTCACATCGTACCACTGGGTGCTGCTGATACTGACGGC
CGCCTGAGGTCTGGAGATGAATTAATCTGTGTGGATGGGACGCCAGTAATTGGAAAATCA
CACCAGCTTGTGGTCCAGCTTATGCAACAAGCTGCCAAGCAAGGCCACGTCAATCTCACG
GTGCGGCGTAAAGTGGTTTTTGCGGTGCCCAAAACCGAGAACGAGGTGCCCTCGCCAGCC
TCCTCTCATCACAGTAGCAACCAGCCGGCCTCGCTGACAGAAGAGAAGCGCACTCCGCAG
GGCAGCCAGAACTCGCTGAACACGGTGAGCTCGGGCAGCGGCAGCACCAGCGGCATCGGC
AGTGGCGGCGGCGGGGGCAGCGGCGTGGTCAGCACCGTGGTGCAGCCCTACGACGTGGAG
ATCCGGCGCGGGGAGAACGAGGGCTTCGGCTTCGTCATCGTGTCCTCGGTGAGCAGGCCC
GAAGCAGGCACGACCTTTGCAGGCAATGCATGTGTGGCTATGCCTCACAAAATAGGTCGG
ATTATTGAGGGGAGCCCTGCTGACCGCTGTGGCAAGCTGAAAGTAGGAGACCGGATCTTG
GCAGTAAATGGATGTTCCATCACCAACAAATCCCATTCAGACATTGTGAACCTAATCAAG
GAAGCGGGAAACACAGTTACCCTCCGCATCATTCCTGGGGATGAGTCCTCGAATGCCACC
TTGCTGACCAATGCAGAGAAGATTGCCACCATCACCACCACACACACCCCTTCTCAGCAA
GGGACCCAGGAGACAAGGAATACCACCAAACCAAAGCAGGAATCTCAATTTGAGTTCAAA
GCACCCCAAGCAACACAGGAGCAAGATTTTTACACTGTGGAACTGGAAAGAGGAGCCAAG
GGATTTGGCTTTAGCCTTCGAGGAGGCCGAGAGTATAACATGGACCTCTATGTTCTGCGC
TTAGCAGAGGACGGTCCTGCGGAGAGGTGTGGAAAGATGAGGATTGGTGATGAAATTTTA
GAGATCAATGGTGAGACCACCAAAAACATGAAGCATTCTCGAGCTATAGAACTGATTAAG
AATGGTGGCCGCAGAGTTCGTCTGTTTCTGAAGCGGGGAGACGGCTCAGTACCAGAATAT
GGTGGGTCAAACTATGAAAACATTCCTTCCTTCCCTGGCATGACTCCATGA
Protein sequence predicted based on nucleotide sequence
MSKVIQKKNHWTSRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAVEAAGLPGGGE GPRLGEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRF QKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYNFLTVKEFLDLEQSGTLLEVG TYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHAENEE EDDVPEMNSSFTADSGEQEEHTLQETALPPVNSSIIAAPITDPSQKFPQYLPLSAEDNLG PLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELE LPAGWEKIEDPVYGIYYVDHINRKTQYENPVLEAKRKKQLEQQQQQQQQQQQQQQQQQQQ QTEEWTEDHSALVPPVIPNHPPSNPEPAREVPLQGKPFFTRNPSELKGKFIHTKLRKSSR GFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQ SIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTG KVNGMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADS PGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQALTHNQVVDMLVECPKGSEVTLLVQ RGGLPVPKKSPKSQPLERKDSQNSSQHSVSSHRSLHTASPSHSTQVLPEFPPAEAQAPDQ TDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGEREREINSTNFGECPIPDYQEQ DIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKS HQLVVQLMQQAAKQGHVNLTVRRKVVFAVPKTENEVPSPASSHHSSNQPASLTEEKRTPQ GSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSTVVQPYDVEIRRGENEGFGFVIVSSVSRP EAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIK EAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHTPSQQGTQETRNTTKPKQESQFEFK APQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEIL EINGETTKNMKHSRAIELIKNGGRRVRLFLKRGDGSVPEYGGSNYENIPSFPGMTP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Confirmed
SolubleProtein: Protein_Confirmed
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
