RD1_3083 (R. denitrificans )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  RD1_3083
Gene Name:  short chain dehydrogenase
Original Clone ID:
RdR78A-20-242-21.45
Keyword:
targeted domain
Species:
Roseobacter denitrificans
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
RdCD00343664
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 672nts
Open reading frame: 1 to 672

ATGGCCCCTATGAGCGAATCCGAGAAGTTGCTATATCGCTCCAACCTTCTGGGACCGGAC
AAGAGAATCACAAATTACGGCGGCGGCAATACATCGGCCAAGGTGATCGAAACGGATCCG
TTGACGGGTGAGCCTGCCGAGGTGCTCTGGGTCAAGGGGTCAGGCGGCGATGTCGGGTCG
GTCAGGATGGACGGGTTCGCGACGCTCTACATGGACAAGCTGAACGCATTACGCGGCATC
TATCGCGGCGGCGAGCATGAGGATGAGATGGTGGCCTTCCTGCGCCATTGCACGTTCAAC
CTGAACCCGCGCGCGGCCAGCATCGACACGCCGCTGCATGCCTATGTGCCGGCAAAACAC
GTCGATCACATGCACCCGGATGCGATTATTGCCATCGCCGCCGCGAAGGACAGCAAGAAA
CTCACCCAAGAGATTTTCGGGGATCGCATCGGGTGGCTGCCTTGGAAAAGACCGGGCTAC
GAACTGGGGCTATGGCTGTCGGATTTCTGCGAAAAGAACCCCGACGCCAAGGGTGTTGTG
CTGGAAAGCCATGGGCTCTTTACCTGGGCCGATGACGCGCAGGAGTGCTATGAACGGACG
CTGGAGATCATTCAGACCGCGATGGATTGGTTTGCAGAGCAAACAGCGGGCAAGCCCGCT
TTTGGCCGCGCA

Protein sequence predicted based on nucleotide sequence

MAPMSESEKLLYRSNLLGPDKRITNYGGGNTSAKVIETDPLTGEPAEVLWVKGSGGDVGS
VRMDGFATLYMDKLNALRGIYRGGEHEDEMVAFLRHCTFNLNPRAASIDTPLHAYVPAKH
VDHMHPDAIIAIAAAKDSKKLTQEIFGDRIGWLPWKRPGYELGLWLSDFCEKNPDAKGVV
LESHGLFTWADDAQECYERTLEIIQTAMDWFAEQTAGKPAFGRA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t55c,S19P;a181g,I61V;
g581a,R194Q;g667c,G223R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in RdR78A
Recommended expression in:
Not Applicable