Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
CpR74B-413-474-21.13
Keyword:
targeted domain
Species:
Clostridium perfringens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
CpCD00343769
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 189nts
Open reading frame: 1 to 189

ATGAATAAACAAGGGGTAGTAAAGGTAAATAGTGCTTTAAATATGAGAAGCGGTCCAGGA
AGTAATTACGGAGTAATAGGAACTTTACGTAATAATGATAAGGTAGAGATAATTAAAGAA
GTAGATGGATGGTATGAAATAAAGTTTAATGGAAAAAGTGGATATGTATCAAGCCAATAC
ATAAAAGTA

Protein sequence predicted based on nucleotide sequence

MNKQGVVKVNSALNMRSGPGSNYGVIGTLRNNDKVEIIKEVDGWYEIKFNGKSGYVSSQY
IKV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c36t,A12A;t51c,S17S;
c54t,G18G;c63t,S21S;
g100a,E34K;a108g,E36E;
c111a,I37I;c114t,I38I;
g117a,K39K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in CpR74B
Recommended expression in:
Not Applicable