rpsA (B. thuringiensis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
BuR149F-21.2
Keyword:
targeted domain
Species:
Bacillus thuringiensis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BtCD00344190
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 207nts
Open reading frame: 1 to 207

ATGGGTCATGTATTTGATGTTACTGTAAAAGATATTGTGAATGGCGGATTAGTTGTGGAC
CTTGGTGTTCGCGGTTTTATTCCAGCTTCACTTGTAGAAGTACATTATGTAGAAGACTTT
ACTGACTATAAAGGTAAAACATTAGCGGTGAAAATTGTTGAATTAGACCGTGAAAAAAAT
CGTGTAATCCTTTCGCATAAAGCGGTA

Protein sequence predicted based on nucleotide sequence

MGHVFDVTVKDIVNGGLVVDLGVRGFIPASLVEVHYVEDFTDYKGKTLAVKIVELDREKN
RVILSHKAV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c36t,I12I;t45c,G15G;
t72c,R24R;c81t,I27I;
g84a,P28P;t117c,D39D;
c135t,G45G;g141a,T47T;
c147g,A49A;c171t,R57R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in BuR149F
Recommended expression in:
Not Applicable