rpsA (B. thuringiensis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
BuR149D-21.2
Keyword:
targeted domain
Species:
Bacillus thuringiensis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BtCD00344191
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 249nts
Open reading frame: 1 to 249

ATGGGTGACGTTGTTACAGGTTCTGTAACGAAGGTTGAAGAGAAACAAGTGCTTGTAAAT
GTTGGATACAAAACAGATGGCGTAATTCCAATTAGTGAATTAGCTAACGTTCATATTGAA
AAAGCAAGCGATGTTGTAGAATTAGATCAAACACTAGAGTTGAAAATTATTAAATTAGAA
GAAGATGATCTTGTCTTATCTAAACGTGCTGTTGATGCAGAAAAAGCATGGGTAGAATTA
CAAGAGAAA

Protein sequence predicted based on nucleotide sequence

MGDVVTGSVTKVEEKQVLVNVGYKTDGVIPISELANVHIEKASDVVELDQTLELKIIKLE
EDDLVLSKRAVDAEKAWVELQEK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a33g,K11K;c60t,N20N;
g99a,E33E;a159g,E53E;
a162g,L54L;g165a,K55K;
g166a,V56I;g183a,E61E;
a184g,N62D;a195c,V65V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in BuR149D
Recommended expression in:
Not Applicable