rpsA (B. thuringiensis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
BuR149E-21.2
Keyword:
targeted domain
Species:
Bacillus thuringiensis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BtCD00344193
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 234nts
Open reading frame: 1 to 234

ATGGTAGAATTACAAGAGAAATTTAATTCTGGTCATGTATTTGATGTTACTGTAAAAGAT
ATTGTGAATGGCGGATTAGTTGTGGACCTTGGTGTTCGCGGTTTTATTCCAGCTTCACTT
GTAGAAGTACATTATGTAGAAGACTTTACTGACTATAAAGGTAAAACATTAGCGGTGAAA
ATTGTTGAATTAGACCGTGAAAAAAATCGTGTAATCCTTTCGCATAAAGCGGTA

Protein sequence predicted based on nucleotide sequence

MVELQEKFNSGHVFDVTVKDIVNGGLVVDLGVRGFIPASLVEVHYVEDFTDYKGKTLAVK
IVELDREKNRVILSHKAV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c63t,I21I;t72c,G24G;
t99c,R33R;c108t,I36I;
g111a,P37P;t144c,D48D;
c162t,G54G;g168a,T56T;
c174g,A58A;c198t,R66R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in BuR149E
Recommended expression in:
Not Applicable