Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH221178.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
VcCD00034732
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2388nts
Open reading frame: 1 to 2388
ATGAAATTCAGCGAATCATGGCTTCGTGAGTGGGTTAACCCGGCAATTACCACTGACGAA
TTAACTCACCAAATTACAATGGCGGGTCTGGAAGTGGATGACGTACTGGCCGTAGCTGGT
GTGTTCGACGGCGTAAAAGTGGGTCACGTGGTTGAGTGTGCTCAACATCCAGATGCTGAC
AAACTGCGCGTCACCAAAGTCGATGTAGGCGAAGAAGAGCTTCTGGACATCGTGTGTGGT
GCAGCCAACTGTCGTCAAGGTCTGAAAGTTGCGGTTGCAACCGTAGGCGCGACGCTGCCA
GGCGATTTCAAGATTAAAAAAGCTAAACTGCGTGGCCAACCGTCACACGGCATGCTGTGT
TCTTTCTCTGAGCTGGGTATCGATGTTGAATCGAACGGCATCATGGAACTGGCTGAAAAC
GCACCGATTGGTATGGATTTCCGCGATTTTCTGTCACTGAACGATGTGACTATCGATGTG
GATTTGACGTCTAACCGCGCGGACTGTTTCAGCATCCGTGGTCTGGCTCGTGAAGTGGGC
GTATTGAACCGTGCGGACGTGACTGCTCCTGCGGTGAATGCGATTGTAGCAACCATCAAT
GATACGATTTCAATTGACGTGAAAGCGCCAGCGGCGTGTCCGCGTTACCTTGGTCGTATC
GTGAAGAACGTGAATGTTCAAGCGCAAACGCCACTCTGGATGCAAGAAAAACTGCGTCGT
TGTGGTATCCGCTCTATCGATCCGGTGGTTGATATCACTAACTTCGTGATGCTGGAGCAA
GGCCAACCAATGCACGCGTTTGATCTGGCGAAGATTGAAGGCGGAATCGTGGTTCGTCTG
GCTGAGCAAGATGAAAAACTGACGCTGCTTGATGGCTCAGAAGCCAAATTAAACGCAGAT
ACGCTAGTGATTGCTGACCAACAAAAAGCGCTGGCGATTGCTGGTGTATTCGGTGGCGAG
CATTCTGGCGTCAGCACTGACACCAAAGATGTATTACTTGAGTGTGCGTTCTTCGCACCA
GACCACATTCGTGGTCGTGCACGTAGCTACGGCCTACACACCGATTCTTCAATGCGTTTT
GAACGTGGTGTGGATTATGCTTTGCAACACGCCGCGATGGAGCGTGCAACGCAACTGCTG
GTTGAGATCTGTGGTGGTGACGTTGCGCCAGTGGTAGCTGCAGAATCTGCGGCCGATTTA
CCAAAACCAAACCAAGTTGCGCTGCGTCGTAGCAAGCTCGACAAACTGCTGGGTCATGCG
ATTCCGGATGCGGACGTCGTGGAGATTCTAGAGCGTCTAGGCATGCAGGTCGAGACCACC
GCAGAAGGCTGGCAAGCGACCGCGCCAACATGGCGTTTTGATATTGCTATCGAGCAAGAT
CTGGTGGAAGAAGTTGGCCGTATCTATGGTTACAACAACATTCCTAATCAAGCGCCAGTG
GCCGCGTTGAACATGAACCTGCACAACGAAGCGAAATTACCGCTGAAGCGTGTACGCGAT
CTGCTGGTTGACCGTGGTTACCAAGAAGCGATCACCTATAGCTTCGTTGAGCCTGAGCAA
CAGAAACTGGTTGTACCGGGTGTTGATGCGCTGATCCTGCCAAACCCAATTTCTGCTGAA
ATGTCAGCGATGCGTTTGAGCTTGATCCAAGGTCTGCTGAACACTGTGGTTCACAACCAG
AAACGTCAGCAACCTCGTGTTCGTTTGTTTGAATACGGTTTGCGCTTCATTCCTGATGCG
GCGGCAGAAAATGGCATGCGCCAAGAGCCTATGCTGGCGGGTGTGATCTCTGGCGCTCGT
GGTGAAGAGCACTGGAATATGGAAACGGCAACCGTTGATTTCTTCGATATGAAGGGCGAT
CTGGAAGCAGTTCTTGAACTGACCGCGAAAGGCAAAGCCTATAGCTTCGCCGCGACTAAG
CACCCAGCGCTGCATCCAGGTCAAGCTGCAGCGATTATGGTGGATGGCAAAGCGATCGGT
GTTATTGGTACGGTTCACCCAGAACTTGAGCGTAAGTTTGGCCTCAATGGTCGCACTATT
GTGTTCGAGATCGAGTGGAATGCGATCAACACACGCGTGATCCCAGAAGCCGCTGCGATT
TCTAAGTTCCCAGCCAACCGTCGTGATATCGCGTTGGTGGTCGATGGCAACATCGCTTCT
GGCGACATCGTTGAAGCTTGCCGTGTGGCGGGTGGTGAGCTACTGAAAGACGCAAAACTG
TTTGACGTTTATGTCGGTAAAGGCGTGGAAGAAGGCAAGAAGAGCTTGGCGATTGCGCTG
ACGTTGCAATCGGTTGAACGCACACTTGAAGAAGCGGATATCGCAGCAGCGGTAGAGGCG
ATTGTGCAAGCCGTATCTGCCCAATTCGGCGCGGCTCTGCGCGACTTG
Protein sequence predicted based on nucleotide sequence
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDAD KLRVTKVDVGEEELLDIVCGAANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLC SFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDVDLTSNRADCFSIRGLAREVG VLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNAD TLVIADQQKALAIAGVFGGEHSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRF ERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADLPKPNQVALRRSKLDKLLGHA IPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQAPV AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAE MSAMRLSLIQGLLNTVVHNQKRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGAR GEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATKHPALHPGQAAAIMVDGKAIG VIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEA IVQAVSAQFGAALRDL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2388
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
