Gene:
Gene Symbol:
TRERF1
Gene Name:
transcriptional regulating factor 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100061587)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00350632
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGTGACCAGCAACTGTACAAGACCAACCATGTGGCCCATGGTAGTGAGAACCTTTTC
TACCAACAGCCACCACTTGGCGTCCACAGCGGGCTGAACCACAACTATGGGAATGCAGTT
ACAGGGGGCGGAATGGATGCCCCTCAGGCCTCGCCAATCTCCCCCCACTTCCCTCAAGAT
ACACGGGATGGTCTGGGCTTGCCTGTTGGCTCCAAAAACCTTGGCCAAATGGATACCTCG
AGGCAGGGAGGGTGGGGAAGTCATGCAGGGCCTGGAAACCATGTCCAGCTACGTGGAAAC
CTGGCCAACTCAAACATGATGTGGGGGGCACCAGCCCAGGCTGAGCCCACTGATGGCTAC
CAATACACCTACTCCCAGGCCAGCGAGATCCGGACCCAGAAGCTTACCAGCGGTGTCTTA
CACAAGCTGGACTCTTTCACCCAGGTGTTTGCCAACCAAAACCTGCGAATTCAGGTCAAC
AATATGGCCCAGGTGCTGCACACTCAGTCAGCAGTGATGGATGGAGCCCCTGACAGTGCT
CTCCGCCAGCTGCTGTCTCAGAAGCCCATGGAGCCCCCAGCACCGGCTATCCCTTCCCGC
TACCAGCAGGTGCCCCAGCAGCCTCACCCTGGTTTCACTGGTGGGCTGTCCAAACCAGCT
CTTCAGGTCGGGCAGCACCCTACCCAAGGGCACCTGTATTATGACTACCAGCAGCCTCTG
GCTCAGGTGCCAGTGCAGGGAGGACAGCCACTGCAGGCCCCACAGATGCTGTCACAGCAC
ATGCAACAGATGCAGCAGCACCAGTATTACCCACCGCAGCAACAGCAGCAAGCCGGGCAA
CAGCGTATCTCCATGCAAGAAATACAGACGCAGCCGCAACAAATTCGCCCATCACAGCCA
CAGCCGCCGCCACAGCAGCAGCAGCCGCAGCAGCTACAGCTGCAGCAGCGGCAGGGTTCA
ATGCAGATACCTCAGTATTATCAGCCCCAACCCATGATGCAGCACTTGCAAGAGCAGCAG
CAGCAACAGATGCACCTGCAGCCTCCTTCTTATCACAGGGACCCTCACCAGTATACCCCA
GAGCAGGCACACACTGTCCAGCTGATTCCCCTGGGCTCCATGTCCCAGTACTACTACCAG
GAGCCCCAGCAGCCCTACAGCCACCCCCTCTACCAGCAGAGCCACCTGTCCCAGCACCAG
CAGCGTGAGGACAGTCAGCTGAAGACCTACTCTAGTGACAGACAGGCCCAGGCCATGCTG
AGCTCCCATGGGGACCTGGGGCCTCCTGACACAGGAATGGGAGACCCAGCGAGCTCAGAT
CTGACCCGGGTCAGCAGCACCCTCCCCCATCGCCCCCTCCTATCCCCCAGTGGGATCCAC
CTCAACAACATGGGGCCTCAGCATCAGCAGCTGTCTCCCAGTGCCATGTGGCCCCAGATG
CACCTACCTGATGGGAGAGCCCAGCCAGGGTCCCCTGAGTCAAGTGGCCAACCCAAAGGA
GCGTTTGGGGAGCAGTTTGATGCCAAGAACAAGCTGACATGCTCCATCTGCCTGAAGGAG
TTCAAGAACCTGCCTGCCCTGAATGGCCACATGCGGTCCCACGGGGGAATGAGGGCCTCC
CCCAACCTCAAACAGGAGGAAGGAGAGAAGGTCCTGCCGCCTCAGCCCCAGCCACCACTG
CCGCCTCCGCCTCCGCCTCCGCCGCCACCACAGCTCCCTCCCGAGGCAGAAAGCCTCACG
CCTATGGTCATGCCCGTGTCTGTCCCTGTCAAGCTTCTCCCGCCCAAGCCCAGCTCTCAG
GGGTTCACCAACAGCACCGTTGCCGCCCCCTCCGCCAGAGACAAGCCAGCCAGCTCGATG
TCGGACGACGAGATGCCTGTGCTCGAAATCCCCAGGAAGCATCAGCCGAGTGTGCCCAAA
GCCGAGGAGCCCCTCAAGACCGTGCAGGAGAAGAAAAAGTTCCGGCACCGGCCGGAACCT
CTCTTCATCCCGCCGCCGCCCTCCTACAACCCGAACCCCGCTGCCTCCTACTCGGGCGCC
ACCCTGTACCAGAGCCAGCTGCGCTCCCCGCGCGTCCTCGGGGACCACCTGCTCCTGGAC
CCCACCCACGAGCTGCCCCCTTACACGCCCCCACCCATGCTGAGCCCGGTGCGCCAGGGC
TCGGGGCTCTTCAGCAATGTCCTCATCTCCGGCCACGGCCCTGGCGCCCACCCGCAGCTG
CCCCTGACGCCCCTGACGCCCACACCACGGGTGCTGCTGTGTCGCTCCAACAGCATCGAT
GGCAGCAACGTGACGGTCACCCCAGGGCCTGGAGAGCAGACTGTAGATGTTGAACCACGC
ATCAACATTGGCTTGAGATTCCAAGCAGAAATCCCTGAACTCCAAGATATCTCTGCCCTG
GCCCAGGACACACACAAGGCCACACTGGTATGGAAGCCCTGGCCAGAACTAGAAAACCAT
GACCTCCAGCAAAGAGTGGAGAATCTTCTGAATTTGTGCTGTTCCAGTGCATTGCCAGGT
GGAGGGACCAATTCTGAATTTGCTTTGCACTCTCTGTTTGAGGCCAAAGGTGATGTGATG
GTTGCTCTGGAAATGCTGCTACTGCGGAAGCCTGTCAGGTTAAAATGTCATCCTTTAGCA
AATTACCACTATGCCGGTTCGGACAAGTGGACCTCCCTAGAAAGAAAACTGTTTAACAAA
GCACTAGCCACTTACAGCAAAGACTTTATTTTTGTACAGAAGATGGTGAAGTCCAAGACG
GTGGCTCAGTGCGTGGAGTACTACTACACGTGGAAAAAGATCATGCGGCTGGGGCGGAAA
CACCGGACACGCCTGGCAGAAATCATCGACGATTGTGTGACAAGTGAAGAAGAAGAAGAG
TTAGAGGAGGAGGAGGAGGAGGACCCGGAAGAAGATAGGAAATCCACAAAAGAAGAAGAG
AGTGAGGTGCCGAAGTCCCCGGAGCCACCACCCGTCCCCGTCCTGGCTCCCACGGAGGGG
CCGCCCCTGCAGGCCCTGGGCCAGCCCTCAGGCTCCTTCATCTGTGAAATGCCCAACTGT
GGGGCTGTGTTCAGCTCCCGACAGGCACTGAATGGCCATGCCCGCATCCACGGGGGCACC
AACCAGGTGACCAAGGCCCGAGGTGCCATCCCCTCTGGGAAGCAGAAGCCTGGTGGCACC
CAGAGTGGGTACTGTTCGGTAAAGAGCTCACCCTCTCACAGCACCACCAGCGGCGAGACA
GACCCCACCACCATCTTCCCCTGCAAGGAGTGTGGCAAAGTCTTCTTCAAGATCAAAAGC
CGAAATGCACACATGAAAACTCACAGGCAGCAGGAGGAACAACAGAGGCAAAAGGCTCAG
AAGGCGGCTTTTGCAGCTGAGATGGCAGCCACGATTGAGAGGACTACGGGGCCCGTGGGG
GCGCCGGGGCTGCTGCCCCTGGACCAGCTGAGTCTGATCAAACCCATCAAGGATGTGGAC
ATCCTCGACGACGACGTCGTCCAGCAGTTGGGAGGTGTCATGGAAGAGGCTGAAGTTGTG
GACACCGATCTTCTCTTGGATGATCAAGATTCAGTCTTGCTTCAGGGTGACGCAGAACTA
Protein sequence predicted based on nucleotide sequence
MGDQQLYKTNHVAHGSENLFYQQPPLGVHSGLNHNYGNAVTGGGMDAPQASPISPHFPQD TRDGLGLPVGSKNLGQMDTSRQGGWGSHAGPGNHVQLRGNLANSNMMWGAPAQAEPTDGY QYTYSQASEIRTQKLTSGVLHKLDSFTQVFANQNLRIQVNNMAQVLHTQSAVMDGAPDSA LRQLLSQKPMEPPAPAIPSRYQQVPQQPHPGFTGGLSKPALQVGQHPTQGHLYYDYQQPL AQVPVQGGQPLQAPQMLSQHMQQMQQHQYYPPQQQQQAGQQRISMQEIQTQPQQIRPSQP QPPPQQQQPQQLQLQQRQGSMQIPQYYQPQPMMQHLQEQQQQQMHLQPPSYHRDPHQYTP EQAHTVQLIPLGSMSQYYYQEPQQPYSHPLYQQSHLSQHQQREDSQLKTYSSDRQAQAML SSHGDLGPPDTGMGDPASSDLTRVSSTLPHRPLLSPSGIHLNNMGPQHQQLSPSAMWPQM HLPDGRAQPGSPESSGQPKGAFGEQFDAKNKLTCSICLKEFKNLPALNGHMRSHGGMRAS PNLKQEEGEKVLPPQPQPPLPPPPPPPPPPQLPPEAESLTPMVMPVSVPVKLLPPKPSSQ GFTNSTVAAPSARDKPASSMSDDEMPVLEIPRKHQPSVPKAEEPLKTVQEKKKFRHRPEP LFIPPPPSYNPNPAASYSGATLYQSQLRSPRVLGDHLLLDPTHELPPYTPPPMLSPVRQG SGLFSNVLISGHGPGAHPQLPLTPLTPTPRVLLCRSNSIDGSNVTVTPGPGEQTVDVEPR INIGLRFQAEIPELQDISALAQDTHKATLVWKPWPELENHDLQQRVENLLNLCCSSALPG GGTNSEFALHSLFEAKGDVMVALEMLLLRKPVRLKCHPLANYHYAGSDKWTSLERKLFNK ALATYSKDFIFVQKMVKSKTVAQCVEYYYTWKKIMRLGRKHRTRLAEIIDDCVTSEEEEE LEEEEEEDPEEDRKSTKEEESEVPKSPEPPPVPVLAPTEGPPLQALGQPSGSFICEMPNC GAVFSSRQALNGHARIHGGTNQVTKARGAIPSGKQKPGGTQSGYCSVKSSPSHSTTSGET DPTTIFPCKECGKVFFKIKSRNAHMKTHRQQEEQQRQKAQKAAFAAEMAATIERTTGPVG APGLLPLDQLSLIKPIKDVDILDDDVVQQLGGVMEEAEVVDTDLLLDDQDSVLLQGDAEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
