Gene:
Gene Symbol:
RAPH1
Gene Name:
Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
clone failed to grow from both plate and tube sources.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00350656
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCAGCTATCAGATGAAGAAATTGATCATGGTGCTGAAGAAGACAGTGACAAGGAA
GATCAGGACCTGGACAAAATGTTTGGAGCCTGGCTTGGAGAACTAGACAAACTCACTCAG
AGTTTGGATTCTGACAAGCCCATGGAACCAGTAAAAAGATCTCCTCTTCGCCAGGAAACA
AACATGGCCAACTTTTCTTACCGCTTCTCCATATACAACTTGAATGAAGCTCTGAATCAG
GGAGAGACTGTGGATCTGGATGCCTTGATGGCTGATCTTTGCTCTATAGAGCAGGAGCTC
AGCAGCATTGGTTCAGGAAACAGTAAGCGTCAAATCACAGAAACGAAAGCTACTCAGAAA
TTGCCTGTTAGCCGACATACATTGAAACATGGCACCTTGAAAGGATTATCTTCTTCATCT
AATAGGATAGCTAAACCTTCCCATGCCAGCTACTCCTTGGACGACGTCACTGCACAGTTA
GAACAGGCCTCTTTGAGTATGGATGAGGCTGCTCAGCAATCTGTACTAGAAGATACTAAA
CCCTTAGTAACTAATCAGCACAGAAGAACCGCGTCAGCAGGCACAGTGAGTGATGCTGAA
GTACACTCTATTAGTAATTCCTCCCATTCCAGCATCACTTCCGCAGCCTCCAGCATGGAC
TCTTTGGATATTGATAAAGTAACACGCCCTCAAGAGCTGGATTTGACACATCAAGGGCAG
CCAATTACTGAGGAAGAACAGGCAGCAAAATTGAAAGCTGAGAAGATCAGAGTTGCCCTA
GAGAAAATTAAAGAGGCACAAGTGAAAAAGCTGGTGATCAGAGTCCACATGTCTGATGAC
AGTTCTAAAACAATGATGGTGGATGAGAGGCAGACAGTAAGACAAGTACTGGATAACCTG
ATGGACAAATCCCACTGCGGTTATAGTTTAGACTGGTCACTGGTAGAAACCGTTTCTGAA
TTACAAATGGAGAGAATCTTTGAAGACCATGAAAACTTGGTTGAAAATCTTCTTAATTGG
ACAAGAGATAGCCAAAACAAGCTTATATTTATGGAGCGTATAGAAAAATATGCACTTTTC
AAAAACCCACAGAATTATCTTTTGGGGAAAAAAGAAACAGCTGAGATGGCAGATAGAAAC
AAAGAAGTCCTCTTGGAGGAATGTTTTTGTGGAAGTTCTGTAACTGTACCAGAAATTGAA
GGAGTCCTTTGGTTGAAGGATGATGGCAAGAAGTCCTGGAAAAAGCGTTATTTTCTCTTG
CGAGCATCTGGTATCTACTATGTTCCCAAAGGAAAAGCAAAGGTCTCTCGGGATCTGGTG
TGCTTTCTCCAGCTGGATCATGTCAACGTTTATTATGGCCAGGACTATCGGAACAAATAC
AAAGCACCTACAGACTATTGTCTGGTGCTGAAGCATCCACAAATCCAGAAGAAATCTCAA
TATATCAAATACCTTTGTTGTGATGATGTGAGGACACTGCATCAGTGGGTCAATGGGATC
CGCATTGCAAAGTATGGGAAGCAGCTCTATATGAACTACCAAGAAGCCTTGAAGAGGACA
GAGTCAGCCTATGATTGGACTTCCTTATCCAGCTCCAGCATTAAATCGGGATCCAGTTCT
TCCAGCATCCCAGAGTCTCAGTCAAACCACTCCAATCAGTCTGATAGCGGAGTTTCTGAC
ACCCAGCCAGCAGGACACGTCCGTTCCCAGAGCATTGTGAGCTCCGTATTCTCTGAAGCC
TGGAAACGAGGCACTCAGTTGGAAGAGTCCAGCAAGGCCAGAATGGAGTCTATGAATCGG
CCCTACACTTCACTTGTGCCCCCTTTATCCCCGCAACCTAAGATAGTCACCCCCTACACT
GCTTCACAGCCTTCACCACCTCTACCTCCTCCGCCACCCCCACCTCCTCCTCCACCACCC
CCTCCACCACCCCCTCCTCCCCCACTCCCCAGCCAGTCTGCACCTTCTGCAGGCTCAGCA
GCCCCAATGTTCGTCAAGTACAGCACAATAACACGGCTACAGAATGCGTCTCAGCATTCA
GGGGCCCTGTTTAAGCCGCCAACACCCCCAGTGATGCAGTCACAGTCAGTGAAGCCTCAG
ATCCTGGTACCCCCCAATGGAGTTGTTCCACCACCCCCTCCCCCTCCTCCACCCCCAACC
CCAGGCTCTGCCATGGCCCAGCTAAAGCCTGCACCGTGTGCCCCATCCCTTCCACAGTTC
AGTGCCCCGCCTCCTCCACTGAAGATCCATCAAGTTCAGCATATTACTCAGGTGGCTCCC
CCAACACCCCCCCCACCTCCTCCTATCCCTGCACCCCTCCCTCCCCAAGCTCCCCCAAAA
CCCCTTGTGACCATCCCCGCACCAACCAGCACCAAGACTGTGGCACCTGTTGTGACTCAA
GCTGCACCACCCACACCTACTCCTCCAGTGCCCCCAGCAAAAAAGCAGCCAGCTTTCCCT
GCTTCTTACATTCCACCCTCTCCCCCTACCCCTCCTGTTCCAGTACCCCCGCCAACATTA
CCCAAGCAACAGAGCTTCTGTGCAAAACCCCCTCCCTCTCCACTGTCACCGGTGCCCTCG
GTCGTGAAGCAGATAGCCAGCCAGTTTCCACCCCCTCCAACTCCCCCTGCCATGGAATCT
CAGCCCTTAAAGCCTGTCCCAGCAAATGTAGCTCCACAGTCCCCTCCTGCAGTAAAAGCA
AAGCCCAAGTGGCAGCCCAGCTCCATCCCAGTCCCTTCTCCGGACTTCCCTCCTCCCCCT
CCTGAAAGCAGCCTGGTGTTTCCTCCTCCACCCCCATCACCTGTCCCAGCCCCACCACCG
CCACCTCCACCCACAGCTTCTCCTACCCCTGACAAAAGTGGATCTCCAGGCAAAAAGACC
AGTAAGACGTCCAGCCCTGGGGGAAAGAAACCACCCCCAACCCCACAGCGCAACTCCAGC
ATTAAATCCAGCAGTGGTGCAGAGCACCCCGAGCCCAAGAGACCCTCGGTGGACAGTCTA
GTCAGCAAGTTTACACCGCCAGCAGAATCAGGGTCTCCCAGCAAGGAGACCCTACCACCT
CCTGCAGCACCCCCCAAGCCTGGAAAACTCAATCTTTCTGGAGTCAACCTTCCTGGAGTT
CTCCAACAAGGGTGTGTGTCAGCAAAAGCCCCTGTTCTGAGTGGGCGTGGAAAGGACTCC
GTGGTGGAATTTCCTTCTCCTCCATCCGATTCTGATTTTCCACCCCCTCCACCTGAAACA
GAGCTTCCTCTGCCCCCCATTGAGATTCCAGCAGTTTTCTCGGGAAACACCTCTCCAAAA
GTGGCAGTCGTTAATCCTCAACCACAACAATGGTCTAAAATGTCAGTGAAGAAGGCCCCT
CCACCCACACGACCCAAACGGAATGATAGCACCCGCCTCACTCAAGCTGAGATTTCTGAG
CAGCCAACAATGGCCACAGTTGTGCCACAAGTGCCCACCTCTCCCAAATCCAGCCTTAGT
GTCCAGCCTGGATTCCTGGCTGACCTCAACAGGACACTGCAACGAAAGTCCATCACTCGG
CACGGCTCACTCTCCTCCCGCATGTCCAGAGCAGAACCAACAGCCACCATGGATGATATG
GCATTGCCTCCACCACCCCCTGAACTGCTGTCTGATCAACAGAAGGCTGGTTACGGAGGC
AGTCATATATCAGGCTATGCAACGTTGCGGAGAGGACCCCCTCCTGCTCCCCCCAAAAGA
GACCAGAACACCAAGCTCTCCAGAGACTGG
Protein sequence predicted based on nucleotide sequence
MEQLSDEEIDHGAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDSDKPMEPVKRSPLRQET NMANFSYRFSIYNLNEALNQGETVDLDALMADLCSIEQELSSIGSGNSKRQITETKATQK LPVSRHTLKHGTLKGLSSSSNRIAKPSHASYSLDDVTAQLEQASLSMDEAAQQSVLEDTK PLVTNQHRRTASAGTVSDAEVHSISNSSHSSITSAASSMDSLDIDKVTRPQELDLTHQGQ PITEEEQAAKLKAEKIRVALEKIKEAQVKKLVIRVHMSDDSSKTMMVDERQTVRQVLDNL MDKSHCGYSLDWSLVETVSELQMERIFEDHENLVENLLNWTRDSQNKLIFMERIEKYALF KNPQNYLLGKKETAEMADRNKEVLLEECFCGSSVTVPEIEGVLWLKDDGKKSWKKRYFLL RASGIYYVPKGKAKVSRDLVCFLQLDHVNVYYGQDYRNKYKAPTDYCLVLKHPQIQKKSQ YIKYLCCDDVRTLHQWVNGIRIAKYGKQLYMNYQEALKRTESAYDWTSLSSSSIKSGSSS SSIPESQSNHSNQSDSGVSDTQPAGHVRSQSIVSSVFSEAWKRGTQLEESSKARMESMNR PYTSLVPPLSPQPKIVTPYTASQPSPPLPPPPPPPPPPPPPPPPPPPPLPSQSAPSAGSA APMFVKYSTITRLQNASQHSGALFKPPTPPVMQSQSVKPQILVPPNGVVPPPPPPPPPPT PGSAMAQLKPAPCAPSLPQFSAPPPPLKIHQVQHITQVAPPTPPPPPPIPAPLPPQAPPK PLVTIPAPTSTKTVAPVVTQAAPPTPTPPVPPAKKQPAFPASYIPPSPPTPPVPVPPPTL PKQQSFCAKPPPSPLSPVPSVVKQIASQFPPPPTPPAMESQPLKPVPANVAPQSPPAVKA KPKWQPSSIPVPSPDFPPPPPESSLVFPPPPPSPVPAPPPPPPPTASPTPDKSGSPGKKT SKTSSPGGKKPPPTPQRNSSIKSSSGAEHPEPKRPSVDSLVSKFTPPAESGSPSKETLPP PAAPPKPGKLNLSGVNLPGVLQQGCVSAKAPVLSGRGKDSVVEFPSPPSDSDFPPPPPET ELPLPPIEIPAVFSGNTSPKVAVVNPQPQQWSKMSVKKAPPPTRPKRNDSTRLTQAEISE QPTMATVVPQVPTSPKSSLSVQPGFLADLNRTLQRKSITRHGSLSSRMSRAEPTATMDDM ALPPPPPELLSDQQKAGYGGSHISGYATLRRGPPPAPPKRDQNTKLSRDW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
