Gene:
Gene Symbol:
ITIH6
Gene Name:
inter-alpha-trypsin inhibitor heavy chain family, member 6
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100061623)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00350688
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTGGGTGGAGGTACCTCATCTGTGTCAGCTTTTTGCTGACCATTCTTCTTGAACTG
ACATACCAGGGACCCCCTGTCCCCGCTTCATCAAGCACAAAGTTGTTAATGACAAGCTAT
TCTATGCGCTCCACGGTGGTGTCTCGCTATGCCCACACCTTGGTCACCTCTGTCCTGTTT
AATCCACATGCTGAAGCCCATGAAGCCATCTTTGACCTGGATCTGCCTCATCTTGCCTTT
ATCTCCAATTTCACTATGACCATCAACAATAAAGTCTACATTGCAGAAGTCAAAGAGAAG
CACCAGGCAAAGAAAATCTATGAAGAAGCCCATCAGCAGGGAAAGACAGCTGCTCATGTA
GGCATCAGGGACCGGGAATCAGAGAAGTTCCGCATCTCCACCAGCCTGGCAGCAGGCACA
GAGGTGACTTTTTCCCTGGCCTATGAGGAACTGCTTCAGCGGCACCAGGGCCAGTACCAG
CTGGTGGTGAGCCTGAGGCCTGGCCAATTGGTGAAGAGGCTGAGCATAGAGGTTACAGTG
TCAGAAAGGACAGGCATCTCCTATGTGCACATACCACCCCTGAGGACCGGCCGTCTGCGC
ACCAATGCCCATGCAAGTGAGGTGGATTCACCCCCATCCACCAGGATCGAGAGGGGAGAG
ACCTGTGTCCGAATCACCTACTGCCCGACATTGCAAGACCAGTCGTCCATCTCTGGGTCA
GGCATCATGGCTGACTTCCTGGTTCAGTACGATGTGGTCATGGAGGACATCATTGGAGAC
GTGCAGATTTACGATGACTATTTCATTCACTACTTTGCCCCCAGAGGCCTTCCACCTATG
GAGAAGAATGTGGTTTTTGTTATTGACGTAAGCAGCTCCATGTTTGGTACCAAGATGGAA
CAGACTAAAACGGCCATGAATGTGATCCTCAGTGACCTTCAAGCCAATGACTACTTCAAC
ATCATCTCCTTTTCTGACACAGTTAATGTTTGGAAAGCTGGAGGCTCAATCCAGGCCACC
ATCCAGAATGTCCACAGTGCCAAGGACTACCTGCATTGCATGGAAGCCGATGGCTGGACA
GACGTCAACTCAGCTCTGCTGGCAGCTGCTTCAGTGCTGAACCATAGCAACCAGGAGCCT
GGGAGGGGCCCCAGTGTGGGGAGGATCCCTCTTATCATCTTCCTGACGGATGGGGAGCCC
ACGGCCGGCGTGACGACCCCCAGTGTGATCCTCTCCAATGTCCGTCAGGCGCTAGGCCAC
AGGGTATCCCTTTTCAGCTTGGCCTTTGGGGATGATGCTGACTTTACACTGCTGCGCCGC
CTGTCCCTGGAAAACCGGGGAATAGCCCGGCGCATATATGAGGACACTGATGCGGCCCTA
CAGCTGAAGGGCCTCTATGAGGAGATCTCCATGCCTCTGCTGGCAGATGTGCGTCTGAAC
TACCTGGGTGGCTTGGTTGGGGCCTCCCCTTGGGCCGTTTTCCCCAACTACTTTGGTGGC
TCTGAGCTGGTGGTGGCAGGACAGGTGCAGCCAGGCAAACAGGAACTGGGCATCCACCTG
GCAGCCCGTGGCCCCAAGGATCAGCTTCTTGTGGCCCACCACAGTGAAGGGGCCACCAAC
AACAGCCAGAAGGCCTTTGGTTGCCCAGGGGAGCCAGCCCCCAATGTGGCCCACTTCATC
CGCCGCCTCTGGGCCTATGTCACCATTGGAGAACTGCTGGATGCACACTTCCAAGCTCGT
GACACCACCACTCGCCACCTGCTGGCTGCCAAAGTCCTCAACCTGTCCCTTGAATACAAC
TTTGTCACACCTCTGACTTCACTGGTCATGGTGCAACCCAAACAGGCCAGTGAGGAGACC
AGGAGACAGACTTCCACCTCTGCTGGGCCAGACACCATCATGCCCTCATCCAGCAGCAGG
CATGGCCTAGGGGTAAGCACAGCTCAGCCAGCCTTGGTGCCCAAGGTCATCTCCCCCAAA
TCAAGGCCTGTGAAACCAAAGTTCTACTTATCCTCAACTACTACTGCCTCTACCAAGAAG
ATGCTAAGTTCCAAAGAGCTGGAGCCATTGGGAGAGAGCCCTCATACCCTGTCAATGCCC
ACATACCCAAAGGCCAAAATTCCAGCACAACAGGATTCTGGCACCTTGGCCCAGCCAACT
CTCAGGACAAAACCTACCATTCTTGTGCCCTCAAATTCTGGTACTCTGTTGCCTCTGAAG
CCCGGCTCTCTATCACACCAGAATCCTGATATATTACCCACGAACTCCAGGACACAAGTC
CCACCTGTGAAACCTGGCATCCCAGCCTCGCCCAAAGCTGACACTGTGAAATGTGTTACT
CCACTGCATTCCAAACCTGGTGCTCCATCGCACCCCCAACTTGGGGCACTCACATCACAG
GCACCTAAAGGCCTGCCACAGTCAAGACCTGGAGTCTCTACACTTCAGGTTCCCAAGTAC
CCACTACACACCAGACCTAGGGTTCCTGCTCCCAAGACCCGAAACAACATGCCACACCTG
GGGCCTGGAATCCTCTTGTCCAAGACCCCTAAAATCTTATTATCTCTTAAACCGAGTGCC
CCACCACACCAAATTTCCACAAGCATATCACTTTCCAAGCCTGAGACCCCAAACCCCCAT
ATGCCTCAAACCCCACTACCTCCTAGACCTGACAGACCAAGGCCCCCACTTCCTGAGAGC
CTAAGCACATTCCCAAATACAATCTCAAGTTCCACAGGTCCCAGCAGTACCACAACCACC
TCTGTCCTTGGAGAACCCCTCCCCATGCCCTTTACTCCCACTCTGCCCCCTGGAAGGTTC
TGGCATCAGTATGACCTCCTCCCGGGTCCCCAGAGGACCAGGCAAGTTCTGGGACCATCT
AGGCCAGGAGTTCCAACAATGAGCCTACTCAACAGCTCCAGGCCTACACCAGAAGGCAGC
CCTCCAAACCTGCCAATCTTGCTGCCTTCTAGCATCCTCCCTGAGGCCATCAGTCTGCTC
CTTCTCCCTGAGGAGCTAGAGCTGCTGTCTGAATCAATGGTGGAATCCAAGTTCGTGGAG
TCCTTGAACCCACCAGCTTTCTATACCTTCCTCACTCCTGATGAAGATGGAAGTCCAAAC
TGGGATGGCAATTCTGAGGAGATCCTGGGAGGAGCTGGAGGCAGCATGGAATCTCAAGGA
AGTTCTGTGGGGTTAGCAAAAGGCACATTGCCTAGCATCTTCACCTTCTCCTCCTCAGTG
GACGGGGACCCCCACTTTGTGATCCAAATCCCACACTCAGAAGAGAAGATCTGCTTCACA
CTGAATGGGCACCCTGGGGACTTGCTGCAGCTCATAGAGGACCCAAAGGCAGGGCTGCAT
GTGAGTGGGAAGCTGCTTGGCGCACCACCAAGGCCGGGCCACAAGGACCAGACTCGCACC
TACTTCCAGATCATCACAGTCACTACAGACAAACCCCGGGCCTATACTATCACCATCAGC
CGCAGTTCTATATCTTTGCGAGGCGAGGGTACCTTGCGCCTGTCCTGGGACCAACCTGCC
CTGCTGAAGAGGCCCCAGCTGGAGCTCTATGTGGCTGCTGCAGCCCGCCTTACCCTCCGC
CTTGGGCCCTACCTTGAGTTCCTAGTCCTCCGACACCGCTACAGGCATCCCAGTACCCTG
CAACTACCCCACCTGGGGTTCTACGTGGCCAATGGCTCAGGCCTCAGCCCCTCAGCCCGT
GGCCTGATAGGGCAGTTCCAGCACGCAGACATCCGACTGGTGACAGGACCTATGGGGCCA
TGCTTACGAAGGCACCATGGCCCAGATGTGCCTGTGATTCTAGGCAAGAGGCTGCTGAAG
GACTCACCAAGGCTGCTGCCCCGCTGGGCTTCCTGCTGGCTGGTGAAGCGCTCTCATGTA
GAGCTGCTTCTGGGCCACCCCTACCTCTCCTATGTCCTG
Protein sequence predicted based on nucleotide sequence
MSGWRYLICVSFLLTILLELTYQGPPVPASSSTKLLMTSYSMRSTVVSRYAHTLVTSVLF NPHAEAHEAIFDLDLPHLAFISNFTMTINNKVYIAEVKEKHQAKKIYEEAHQQGKTAAHV GIRDRESEKFRISTSLAAGTEVTFSLAYEELLQRHQGQYQLVVSLRPGQLVKRLSIEVTV SERTGISYVHIPPLRTGRLRTNAHASEVDSPPSTRIERGETCVRITYCPTLQDQSSISGS GIMADFLVQYDVVMEDIIGDVQIYDDYFIHYFAPRGLPPMEKNVVFVIDVSSSMFGTKME QTKTAMNVILSDLQANDYFNIISFSDTVNVWKAGGSIQATIQNVHSAKDYLHCMEADGWT DVNSALLAAASVLNHSNQEPGRGPSVGRIPLIIFLTDGEPTAGVTTPSVILSNVRQALGH RVSLFSLAFGDDADFTLLRRLSLENRGIARRIYEDTDAALQLKGLYEEISMPLLADVRLN YLGGLVGASPWAVFPNYFGGSELVVAGQVQPGKQELGIHLAARGPKDQLLVAHHSEGATN NSQKAFGCPGEPAPNVAHFIRRLWAYVTIGELLDAHFQARDTTTRHLLAAKVLNLSLEYN FVTPLTSLVMVQPKQASEETRRQTSTSAGPDTIMPSSSSRHGLGVSTAQPALVPKVISPK SRPVKPKFYLSSTTTASTKKMLSSKELEPLGESPHTLSMPTYPKAKIPAQQDSGTLAQPT LRTKPTILVPSNSGTLLPLKPGSLSHQNPDILPTNSRTQVPPVKPGIPASPKADTVKCVT PLHSKPGAPSHPQLGALTSQAPKGLPQSRPGVSTLQVPKYPLHTRPRVPAPKTRNNMPHL GPGILLSKTPKILLSLKPSAPPHQISTSISLSKPETPNPHMPQTPLPPRPDRPRPPLPES LSTFPNTISSSTGPSSTTTTSVLGEPLPMPFTPTLPPGRFWHQYDLLPGPQRTRQVLGPS RPGVPTMSLLNSSRPTPEGSPPNLPILLPSSILPEAISLLLLPEELELLSESMVESKFVE SLNPPAFYTFLTPDEDGSPNWDGNSEEILGGAGGSMESQGSSVGLAKGTLPSIFTFSSSV DGDPHFVIQIPHSEEKICFTLNGHPGDLLQLIEDPKAGLHVSGKLLGAPPRPGHKDQTRT YFQIITVTTDKPRAYTITISRSSISLRGEGTLRLSWDQPALLKRPQLELYVAAAARLTLR LGPYLEFLVLRHRYRHPSTLQLPHLGFYVANGSGLSPSARGLIGQFQHADIRLVTGPMGP CLRRHHGPDVPVILGKRLLKDSPRLLPRWASCWLVKRSHVELLLGHPYLSYVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
