Gene:
Gene Symbol:
MTMR3
Gene Name:
myotubularin related protein 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100000382)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351078
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGATGAAGAGACTCGGCACAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGG
AAGCAGCTGATCCGGGAGGATGAGAATCTTCAGGTTCCTTTCCTTGAACTTCATGGAGAG
AGCACAGAGTTTGTGGGCCGTGCCGAGGATGCCATCATTGCCCTTTCCAATTACAGACTT
CACATCAAGTTCAAGGAGTCTCTTGTTAATGTTCCATTACAGCTTATAGAAAGTGTTGAA
TGCCGAGATATATTTCAGCTTCATTTGACTTGCAAAGACTGCAAAGTTATCAGGTGTCAG
TTTTCAACCTTTGAGCAGTGTCAAGAGTGGCTGAAGAGACTGAACAACGCAATCCGACCA
CCTGCTAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTAT
GCCAGTGAAAAAGAGCAACATGGAGACCTGTGCAGACCAGGGGAGCATGTAACTTCAAGG
TTTAAAAACGAGGTGGAGAGGATGGGTTTTGATATGAACAACGCCTGGAGGATTTCCAAC
ATCAATGAGAAGTACAAATTATGTGGTAGCTATCCTCAAGAGCTCATAGTGCCTGCCTGG
ATCACTGACAAAGAACTGGAAAGTGTATCAAGTTTCAGGTCCTGGAAGCGCATCCCTGCC
GTCATCTACAGGCACCAGAGCAATGGAGCTGTCATTGCCCGCTGTGGACAGCCAGAGGTT
AGCTGGTGGGGCTGGCGAAATGCAGATGATGAGCATCTGGTACAGTCAGTAGCCAAAGCT
TGTGCCTCTGACTCCCGATCGAGTGGCAGCAAGCTGTCAACTAGGAACACTTCTCGAGAC
TTTCCCAATGGGGGAGACCTTTCTGACGTGGAGTTCGATTCTTCTCTGTCAAATGCTTCA
GGAGCAGAGAGTTTAGCCATCCAACCGCAGAAGCTTTTGATCTTGGATGCACGCTCCTAT
GCAGCTGCTGTGGCAAACCGAGCTAAAGGAGGAGGCTGCGAATGCCCAGAGTATTACCCA
AACTGTGAAGTTGTGTTTATGGGGATGGCAAACATTCATTCTATTCGGAGGAGTTTTCAG
TCTCTGCGGTTGCTGTGCACTCAGATGCCAGATCCGGGAAATTGGCTATCAGCTCTTGAA
AGCACAAAATGGCTCCATCACTTGTCTGTGCTTCTGAAATCAGCGCTTCTGGTAGTGCAT
GCTGTGGATCAGGATCAGCGGCCGGTGCTAGTACACTGCTCAGATGGCTGGGACCGCACC
CCCCAGATTGTGGCATTGGCTAAGCTCTTGCTGGACCCTTATTACCGAACCATAGAGGGT
TTCCAGGTCCTCGTGGAAATGGAGTGGCTGGATTTTGGCCATAAATTTGCTGACCGGTGT
GGTCATGGGGAGAACTCGGATGATCTGAATGAACGTTGCCCAGTGTTTCTGCAGTGGCTT
GACTGTGTTCATCAGCTTCAGAGGCAATTTCCTTGCTCTTTTGAGTTCAATGAAGCATTC
CTTGTGAAACTGGTGCAGCATACCTATTCCTGCCTGTTTGGAACATTCCTGTGCAACAAC
GCCAAGGAGAGAGGGGAAAAGCATACTCAGGAACGGACATGTTCCGTGTGGTCACTTCTT
CGGGCAGGCAACAAGGCTTTCAAAAACCTACTGTATTCCTCTCAGTCAGAAGCCGTGCTG
TACCCTGTGTGCCATGTGCGTAACCTGATGCTGTGGAGTGCAGTGTACCTGCCCTGCCCA
TCCCCAACCACCCCTGTGGACGACAGCTGTGCACCATACCCAGCCCCAGGCACCAGCCCT
GATGATCCCCCCCTGAGCCGGCTACCAAAGACTAGATCATACGACAATCTGACCACAGCC
TGTGACAACACAGTGCCTCTGGCCAGCCGGCGCTGCAGCGACCCCAGCCTGAACGAGAAG
TGGCAGGAGCACCGGCGCTCACTAGAGCTGAGCAGCCTGGCTGGCCCTGGAGAGGATCCC
CTTTCTGCCGACAGCCTAGGGAAGCCCACCAGAGTGCCGGGGGGTGCCGAGCTTTCTGTT
GCAGCCGGAGTAGCTGAGGGGCAGATGGAGAACATCTTGCAGGAGGCCACCAAAGAGGAG
AGTGGAGTAGAGGAACCTGCCCACAGGGCAGGCATTGAGATACAGGAGGGTAAAGAGGAC
CCTCTCTTAGAAAAGGAGAGCAGGAGGAAGACACCTGAGGCCTCAGCCATTGGACTTCAC
CAAGACCCAGAACTGGGTGATGCTGCTCTGAGGAGCCATCTGGATATGAGCTGGCCTCTG
TTCTCACAGGGCATTTCTGAACAGCAGAGTGGGCTCAGTGTTCTCCTCAGTTCTCTCCAG
GTCCCCCCCAGGGGAGAGGATTCCCTGGAGGTCCCTGTGGAGCAGTTTCGAATAGAAGAG
ATTGCAGAGGGTAGGGAGGAAGCAGTTCTTCCAATCCCAGTAGATGCAAAAGTTGGCTAT
GGTACCTCACAGTCATGTTCTCTGCTACCTTCCCAAGTCCCTTTTGAGACCAGAGGACCA
AACGTGGACAGTTCTACAGACATGTTAGTGGAAGATAAGGTGAAGTCAGTAAGTGGGCCC
CAAGGTCATCATAGATCTTGCCTTGTAAATAGTGGCAAGGACAGGCTTCCTCAGACCATG
GAACCCAGCCCTTCAGAGACAAGCCTGGTCGAGAGGCCCCAAGTGGGGTCTGTGGTGCAT
AGGACTTCCCTTGGCAGCACTCTCAGCCTGACACGTTCCCCTTGTGCCTTGCCTTTAGCC
GAATGTAAAGAGGGGCTTGTGTGCAATGGTGCCCCAGAGACTGAAAACAGGGCCTCAGAG
CAGCCCCCAGGTCTTAGCACCCTCCAGATGTACCCCACACCCAATGGGCATTGCGCCAAT
GGGGAGGCTGGTAGGAGCAAGGACTCACTGAGCCGTCAGCTGTCTGCTATGAGCTGCAGC
TCTGCCCACTTACACTCAAGGAACTTGCACCACAAGTGGCTGCATAGCCACTCAGGAAGG
CCATCTGCAACCAGCAGCCCCGACCAGCCTTCCCGCAGCCACCTGGACGATGATGGCATG
TCAGTGTACACAGACACGATCCAACAGCGCCTGCGTCAGATTGAGTCAGGCCACCAGCAG
GAAGTAGAAACTTTGAAGAAACAAGTCCAGGAGCTGAAGAGTCGCCTGGAGAGCCAGTAC
CTGACCAGCTCCCTACACTTTAATGGAGACTTTGGGGATGAGGTGACTTCAATCCCCGAC
TCGGAAAGCAATCTGGATCAGAACTGTTTGTCTCGCTGCAGCACAGAGATTTTCTCTGAA
GCCAGCTGGGAGCAGGTGGATAAACAGGACACAGAGATGACCCGTTGGCTTCCTGACCAC
CTGGCCGCCCACTGCTATGCGTGCGACAGTGCCTTCTGGCTTGCCAGCAGGAAGCACCAC
TGCAGGAATTGTGGGAACGTATTCTGCTCCAGTTGTTGTAACCAGAAGGTTCCAGTTCCC
AGCCAGCAGCTCTTTGAACCCAGTCGAGTATGCAAGTCTTGCTATAGCAGCCTACATCCC
ACAAGCTCCAGCATTGACCTTGAACTGGATAAGCCCATTGCTGCCACTTCCAACTGA
Protein sequence predicted based on nucleotide sequence
MDEETRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEFVGRAEDAIIALSNYRL HIKFKESLVNVPLQLIESVECRDIFQLHLTCKDCKVIRCQFSTFEQCQEWLKRLNNAIRP PAKIEDLFSFAYHAWCMEVYASEKEQHGDLCRPGEHVTSRFKNEVERMGFDMNNAWRISN INEKYKLCGSYPQELIVPAWITDKELESVSSFRSWKRIPAVIYRHQSNGAVIARCGQPEV SWWGWRNADDEHLVQSVAKACASDSRSSGSKLSTRNTSRDFPNGGDLSDVEFDSSLSNAS GAESLAIQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMANIHSIRRSFQ SLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVHAVDQDQRPVLVHCSDGWDRT PQIVALAKLLLDPYYRTIEGFQVLVEMEWLDFGHKFADRCGHGENSDDLNERCPVFLQWL DCVHQLQRQFPCSFEFNEAFLVKLVQHTYSCLFGTFLCNNAKERGEKHTQERTCSVWSLL RAGNKAFKNLLYSSQSEAVLYPVCHVRNLMLWSAVYLPCPSPTTPVDDSCAPYPAPGTSP DDPPLSRLPKTRSYDNLTTACDNTVPLASRRCSDPSLNEKWQEHRRSLELSSLAGPGEDP LSADSLGKPTRVPGGAELSVAAGVAEGQMENILQEATKEESGVEEPAHRAGIEIQEGKED PLLEKESRRKTPEASAIGLHQDPELGDAALRSHLDMSWPLFSQGISEQQSGLSVLLSSLQ VPPRGEDSLEVPVEQFRIEEIAEGREEAVLPIPVDAKVGYGTSQSCSLLPSQVPFETRGP NVDSSTDMLVEDKVKSVSGPQGHHRSCLVNSGKDRLPQTMEPSPSETSLVERPQVGSVVH RTSLGSTLSLTRSPCALPLAECKEGLVCNGAPETENRASEQPPGLSTLQMYPTPNGHCAN GEAGRSKDSLSRQLSAMSCSSAHLHSRNLHHKWLHSHSGRPSATSSPDQPSRSHLDDDGM SVYTDTIQQRLRQIESGHQQEVETLKKQVQELKSRLESQYLTSSLHFNGDFGDEVTSIPD SESNLDQNCLSRCSTEIFSEASWEQVDKQDTEMTRWLPDHLAAHCYACDSAFWLASRKHH CRNCGNVFCSSCCNQKVPVPSQQLFEPSRVCKSCYSSLHPTSSSIDLELDKPIAATSN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
