Gene:
Gene Symbol:
ANKS1B
Gene Name:
ankyrin repeat and sterile alpha motif domain containing 1B
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100063941)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351284
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGAAGGACCAGGAGCTGCTGGAAGCTGCTCGCACTGGAAATGTGGCTCTGGTGGAG
AAACTCCTGTCTGGCAGGAAAGGAGGGATCCTGGGCGGTGGATCCGGACCCCTGCCCCTG
TCTAATCTGCTAAGCATCTGGCGAGGCCCCAATGTGAACTGCACAGACAGTTCGGGTTAC
ACTGCTTTACACCACGCAGCCTTAAATGGACATAAGGACATAGTTCTCAAACTACTTCAG
TATGAGGCATCAACAAATGTAGCAGACAACAAAGGGTATTTTCCTATTCACCTGGCTGCC
TGGAAAGGAGATGTGGAAATTGTGAAGATTCTTATTCATCATGGACCATCACATTCCAGG
GTCAATGAACAGAACAATGAAAATGAAACTGCCCTACACTGTGCAGCTCAATATGGACAC
TCAGAAGTAGTTGCTGTTCTCCTAGAAGAGCTCACTGACCCGACAATTAGAAATAGCAAG
CTGGAAACACCTTTGGACTTGGCGGCACTCTACGGACGGCTTAGAGTGGTAAAAATGATC
ATCAGTGCACATCCTAACTTAATGAGCTGCAACACTCGCAAGCACACGCCACTTCACCTT
GCTGCGCGCAATGGCCACAAAGCAGTCGTGCAGGTGCTGCTGGAGGCAGGAATGGATGTG
AGCTGTCAAACAGAAAAGGGGAGTGCACTTCATGAAGCAGCTTTGTTTGGAAAGGTGGAT
GTTGTACGAGTTCTGTTAGAAACAGGAATTGATGCCAACATAAAGGATAGCTTAGGTAGA
ACTGTCTTGGACATTCTGAAAGAACATCCATCTCAGAAATCTCTCCAGATTGCAACACTC
TTACAAGAGTATTTAGAAGGCGTGGGAAGATCTACAGTCCTCGAAGAGCCTGTACAGGAA
GATGCAACACAAGAAACACACATTTCATCTCCTGTTGAGTCTCCTTCCCAAAAGACCAAA
AGTGAAACCGTCACTGGAGAATTATCAAAACTCTTGGATGAAATAAAACTCTGTCAAGAA
AAGGATTATTCGTTTGAAGACTTGTGCCACACAATATCAGACCACTACTTAGATAATTTG
AGCAAGATTTCAGAGGAAGAACTTGGGAAAAATGGAAGCCAGAGTGTAAGAACCTCATCT
ACAATCAATTTGTCACCAGGAGAAGTGGAAGAAGAGGATGATGATGAAAATACGTGTGGG
CCATCAGGACTTTGGGAAGCATTAACTCCGTGTAATGGATGTAGGAACCTTGGCTTCCCC
ATGCTTGCCCAGGAGTCCTATCCAAAGAAGAGAAATTACACTATGGAAATTGTACCATCT
GCTTCTCTGGATACATTTCCTTCAGAAAATGAGAACTTTCTGTGTGATCTCATGGACACA
GCTGTTACAAAGAAACCTTGCTCCTTAGAAATTGCAAGGGCACCTTCCCCAAGAACTGAT
AATGCCTCTGAGGTAGCAGTTACTACTCCAGGAACTAGTAACCATAGAAACAGCTCAACA
GGCCCAACACCTGATTGTTCACCTCCATCCCCTGATACTGCCCTCAAAAATATTGTAAAA
GTCATTCGACCCCAGCCTAAACAGCGAACATCCATTGTGTCTTCTCTGGATTTTCACCGA
ATGAATCACAACCAAGAATATTTTGAAATCAACACATCTACAGGGTGCACAAGCTTTACT
GCCAGTCCTCCTGCTAGTCCACCCACCTCTTCTGTGGGAACCACAGAAGTCAAGAATGAG
GGAACTAACCATACAGATGACCTCTCCCGACAGGATGACAATGATCCCCCAAAAGAATAT
GATCCTGGGCAATTTGCAGGCCTGCTCCATGGATCCTCTCCAGCCTGTGAGTCCCCTGAA
AATCCATTTCATCTCTATGGGAAAAGAGAACAATGTGAAAAAGGACAAGATGAAGTCAGT
TTGGCAAACAGTCCTTTGCCTTTCAAGCAGTCTCCAATAGAAAATAACTCAGAACCTTTG
GTAAAGAAAATTAAACCCAAAGTGGTCAGTAGAACAATTTTTCACAAAAAAAGCAACCAA
CTCGAAAACCATACCATTGTTGGCACAAGATCAACCAGGAGTGGATCTCGGAATGGGGAC
CAGTGGGTTATGAACGCAGGGGGATTTGTGGAGAGAGCCTGTACTCTGGGGAGAATAAGG
TCATTGCCTAAAGCCTTGATCGACATGCATTTGTCAAAAAGTGTCTCTAAATCTGATTCT
GATCTCATTGCCTATCCTTCCAATGAGAAAACATCAAGAGTTAACTGGAGTGAATCTTCC
ACTGCTGAACACAGTTCTAAAGGGAATTCTGAAAGAACACCATCCTTCACATCGGAATGG
GAAGAAATTGACAAAATAATGAGTTCCATAGATGTTGGAATCAACAACGAACTTAAAGAG
ATGAATGGTGAGACCACAAGACCCAGATGCCCTGTCCAAACAGTGGGACAATGGTTGGAA
AGCATTGGGCTACCTCAGTACGAGAACCACCTGATGGCTAATGGATTTGACAATGTGCAG
TTTATGGGAAGCAATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAATTCT
GGGCACAGACAAAGAATTCTACAGGCAATCCAGCTCCTTCCAAAGATGAGACCCATTGGG
CATGATGGCTACCATCCCACCTCTGTAGCTGAGTGGCTGGATTCCATTGAACTGGGCGAC
TACACCAAAGCCTTTCTAATTAATGGCTACACTTCGATGGACCTGTTGAAAAAAATCTGG
GAGGTTGAACTTATTAATGTTTTAAAAATCAATTTGATTGGCCACAGGAAACGTATTTTG
GCATCTCTGGGAGACAGGCTGCACGACGATCCCCCACAGAAGCCCCCTCGGTCCATCACC
CTCAGGGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGC
ACTTACACCACTGGTGGCTCCCTAGACGTTCCTCACATTATCATGCAGGGCGATGCAAGG
AGGAGAAGAAATGAAAACTACTTTGATGATATTCCCCGATCAAAACTGGAGAGGCAGATG
GCTCAGACAGGAGACTGGGGAGAACCTTCCATTACCTTGCGACCTCCGAATGAAGCCACA
GCCTCTACCCCGGTACAGTACTGGCAGCATCACCCAGAAAAGCTTATCTTCCAGTCGTGT
GATTACAAAGCTTTTTATTTAGGTTCTATGCTGATAAAAGAGCTTAGGGGGACAGAATCA
ACCCAAGATGCTTGTGCAAAAATGCGGGCTAACTGTCAGAAGTCTACAGAGCAAATGAAG
AAGGTCCCTACTATTATTCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCAACA
AATAAGAACATAATTGCTGAGCATGAAATTCGTAATATCTCCTGTGCTGCCCAGGACCCA
GAAGACCTCTCAACATTTGCCTATATCACAAAAGATTTGAAGTCTAATCACCACTACTGT
CATGTGTTTACTGCCTTTGATGTGAATTTAGCCTATGAAATCATCCTAACCCTGGGACAG
GCATTCGAAGTCGCTTACCAGCTAGCACTACAAGCAAGAAAAGGGGGACACTCCTCCACA
CTTCCAGAAAGCTTTGAAAACAAACCCTCCAAACCCATCCCCAAGCCCCGCGTTAGCATT
CGCAAGTCCGTGGATCTTCTTCATGCCAGCCATACCGGGCAGGAACCATCAGAGAGACAC
ACTGAGGAGGCACTCAGAAAATTT
Protein sequence predicted based on nucleotide sequence
MGKDQELLEAARTGNVALVEKLLSGRKGGILGGGSGPLPLSNLLSIWRGPNVNCTDSSGY TALHHAALNGHKDIVLKLLQYEASTNVADNKGYFPIHLAAWKGDVEIVKILIHHGPSHSR VNEQNNENETALHCAAQYGHSEVVAVLLEELTDPTIRNSKLETPLDLAALYGRLRVVKMI ISAHPNLMSCNTRKHTPLHLAARNGHKAVVQVLLEAGMDVSCQTEKGSALHEAALFGKVD VVRVLLETGIDANIKDSLGRTVLDILKEHPSQKSLQIATLLQEYLEGVGRSTVLEEPVQE DATQETHISSPVESPSQKTKSETVTGELSKLLDEIKLCQEKDYSFEDLCHTISDHYLDNL SKISEEELGKNGSQSVRTSSTINLSPGEVEEEDDDENTCGPSGLWEALTPCNGCRNLGFP MLAQESYPKKRNYTMEIVPSASLDTFPSENENFLCDLMDTAVTKKPCSLEIARAPSPRTD NASEVAVTTPGTSNHRNSSTGPTPDCSPPSPDTALKNIVKVIRPQPKQRTSIVSSLDFHR MNHNQEYFEINTSTGCTSFTASPPASPPTSSVGTTEVKNEGTNHTDDLSRQDDNDPPKEY DPGQFAGLLHGSSPACESPENPFHLYGKREQCEKGQDEVSLANSPLPFKQSPIENNSEPL VKKIKPKVVSRTIFHKKSNQLENHTIVGTRSTRSGSRNGDQWVMNAGGFVERACTLGRIR SLPKALIDMHLSKSVSKSDSDLIAYPSNEKTSRVNWSESSTAEHSSKGNSERTPSFTSEW EEIDKIMSSIDVGINNELKEMNGETTRPRCPVQTVGQWLESIGLPQYENHLMANGFDNVQ FMGSNVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGD YTKAFLINGYTSMDLLKKIWEVELINVLKINLIGHRKRILASLGDRLHDDPPQKPPRSIT LREPSGNHTPPQLSPSLSQSTYTTGGSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQM AQTGDWGEPSITLRPPNEATASTPVQYWQHHPEKLIFQSCDYKAFYLGSMLIKELRGTES TQDACAKMRANCQKSTEQMKKVPTIILSVSYKGVKFIDATNKNIIAEHEIRNISCAAQDP EDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLALQARKGGHSST LPESFENKPSKPIPKPRVSIRKSVDLLHASHTGQEPSERHTEEALRKF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
