MFI2 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MFI2
Gene Name:  antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100016036)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351413
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2214nts
Open reading frame: 1 to 2214

ATGCGGGGTCCGAGCGGGGCTCTGTGGCTGCTCCTGGCTCTGCGCACCGTGCTCGGTGGC
ATGGAGGTGCGGTGGTGCGCCACCTCGGACCCAGAGCAGCACAAGTGCGGCAACATGAGC
GAGGCCTTCCGGGAAGCGGGCATCCAGCCCTCCCTCCTCTGCGTCCGGGGCACCTCCGCC
GACCACTGCGTCCAGCTCATCGCGGCCCAGGAGGCTGACGCCATCACTCTGGATGGAGGA
GCCATCTATGAGGCGGGAAAGGAGCACGGCCTGAAGCCGGTGGTGGGCGAAGTGTACGAT
CAAGAGGTCGGTACCTCCTATTACGCCGTGGCTGTGGTCAGGAGGAGCTCCCATGTGACC
ATTGACACCCTGAAAGGCGTGAAGTCCTGCCACACGGGCATCAATCGCACAGTGGGCTGG
AACGTGCCCGTGGGCTACCTGGTGGAGAGCGGCCGCCTCTCGGTGATGGGCTGCGATGTA
CTCAAAGCTGTCAGCGACTATTTTGGGGGCAGCTGCGTCCCGGGGGCAGGAGAGACCAGT
TACTCTGAGTCCCTCTGTCGCCTCTGCAGGGGTGACAGCTCTGGGGAAGGGGTGTGTGAC
AAGAGCCCCCTGGAGAGATACTACGACTACAGCGGGGCCTTCCGGTGCCTGGCGGAAGGG
GCAGGGGACGTGGCTTTTGTGAAGCACAGCACGGTACTGGAGAACACGGATGGGAAGACG
CTTCCCTCCTGGGGCCAGGCCCTGCTGTCACAGGACTTCGAGCTGCTGTGCCGGGATGGT
AGCCGGGCCGATGTCACCGAGTGGAGGCAGTGCCATCTGGCCCGGGTGCCTGCTCACGCC
GTGGTGGTCCGGGCCGACACAGATGGGGGCCTCATCTTCCGGCTGCTCAACGAAGGCCAG
CGTCTGTTCAGCCACGAGGGCAGCAGCTTCCAGATGTTCAGCTCTGAGGCCTATGGCCAG
AAGGATCTACTCTTCAAAGACTCTACCTCGGAGCTTGTGCCCATCGCCACACAGACCTAT
GAGGCGTGGCTGGGCCATGAGTACCTGCACGCCATGAAGGGTCTGCTCTGTGACCCCAAC
CGGCTGCCCCCCTACCTGCGCTGGTGTGTGCTCTCCACTCCCGAGATCCAGAAGTGTGGA
GACATGGCCGTGGCCTTCCGCCGGCAGCGGCTCAAGCCAGAGATCCAGTGCGTGTCAGCC
AAGTCCCCCCAACACTGCATGGAGCGGATCCAGGCTGAGCAGGTCGACGCTGTGACCCTG
AGTGGCGAGGACATTTACACGGCGGGGAAGACGTACGGCCTGGTTCCCGCAGCCGGGGAG
CACTATGCCCCGGAAGACAGCAGCAACTCGTACTACGTGGTGGCCGTGGTGAGACGGGAC
AGCTCCCACGCCTTCACCTTGGATGAGCTTCGGGGCAAGCGCTCCTGCCACGCCGGTTTC
GGCAGCCCTGCAGGCTGGGATGTCCCCGTGGGTGCCCTTATTCAGAGAGGCTTCATCCGG
CCCAAGGACTGTGACGTCCTCACAGCAGTGAGCGAGTTCTTCAATGCCAGCTGCGTGCCC
GTGAACAACCCCAAGAACTACCCCTCCTCGCTGTGTGCACTGTGCGTGGGGGACGAGCAG
GGCCGCAACAAGTGTGTGGGCAACAGCCAGGAGCGGTATTACGGCTACCGCGGCGCCTTC
AGGTGCCTGGTGGAGAATGCGGGTGACGTTGCCTTCGTCAGGCACACAACCGTCTTTGAC
AACACAAACGGCCACAATTCCGAGCCCTGGGCTGCTGAGCTCAGGTCAGAGGACTATGAA
CTGCTGTGCCCCAACGGGGCCCGAGCCGAGGTGTCCCAGTTTGCAGCCTGCAACCTGGCA
CAGATACCACCCCACGCCGTGATGGTCCGGCCCGACACCAACATCTTCACCGTGTATGGA
CTGCTGGACAAGGCCCAGGACCTGTTTGGAGACGACCACAATAAGAACGGGTTCAAAATG
TTCGACTCCTCCAACTATCATGGCCAAGACCTGCTTTTCAAGGATGCCACCGTCCGGGCG
GTGCCTGTCGGAGAGAAAACCACCTACCGCGGCTGGCTGGGGCTGGACTACGTGGCGGCG
CTGGAAGGGATGTCGTCTCAGCAGTGCTCGGGCGCAGCGGCCCCGGCGCCCGGGGCGCCC
CTGCTCCCGCTGCTGCTGCCCGCCCTCGCCGCCCGCCTGCTCCCGCCCGCCCTC

Protein sequence predicted based on nucleotide sequence

MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSA
DHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVT
IDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETS
YSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKT
LPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQ
RLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPN
RLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTL
SGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGF
GSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQ
GRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYE
LLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKM
FDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQCSGAAAPAPGAP
LLPLLLPALAARLLPPAL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable