DNASU Plasmid | RNF144B (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RNF144B

Gene Name: ring finger protein 144B

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100067166)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351586

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 909nts

Open reading frame: 1 to 909

Reference Sequence Alignment

ATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCCTGGA
GACCTGGCTCCGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCTGGAC
AAGATGACCACACTCCAGGAATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACAGTAC
ATGCAGCTGGCAATCCGAGAAGGATGTGGGTCTCCCATCACTTGCCCTGACATGGTGTGC
CTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTTTGGTACCTGTGGACCAGTTT
CAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTACCGAACATGG
TGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACAGCCT
GTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGCTTGG
CATGCAGAGGTCTCCTGTAGAGACAGTCAGCCTATTGTCCTGCCAACAGAGCACCGAGCC
CTCTTTGGGACAGATGCAGAAGCCCCCATTAAGCAGTGCCCAGTTTGCCGGGTTTATATC
GAACGCAATGAAGGCTGCGCTCAGATGATGTGCAAAAACTGCAAGCATACATTTTGCTGG
TACTGCCTCCAGAACTTGGATAATGACATTTTCCTCAGACATTATGACAAAGGGCCATGC
AGGAATAAACTTGGCCACTCAAGAGCATCAGTGATGTGGAACCGAACACAGGTGGTGGGG
ATTCCCGTAGGCTTGGGCATCATTGCCTTGGTTACTTCACCCTTGTTACTCCTGGCCTCC
CCATGTATAATCTGTTGTGTCTGCAAGTCCTGTCGGGGCAAGAAGAAAAAGCACGACCCA
TCCACAACC

Protein sequence predicted based on nucleotide sequence

MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQY
MQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTW
CPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRA
LFGTDAEAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPC
RNKLGHSRASVMWNRTQVVGIPVGLGIIALVTSPLLLLASPCIICCVCKSCRGKKKKHDP
STT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

RNF144B

Symbol Nomenclature :

RNF144B

SYNONYM :

IBRDC2; PIR2; bA528A10.3; p53RFP

Designation :

E3 ubiquitin-protein ligase RNF144B|IBR domain containing 2|IBR domain-containing protein 2|p53-inducible RING finger protein

Full Nomenclature :

ring finger protein 144B

GENEID :

255488

GenBank Accession :

564193

HGNC :

21578

Vega :

OTTHUMG00000014322

Target GenBank:

EU832137

Reference Sequence Alignment

  

HsCD00351586      ATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCCTGGA
NM_182757.3       ATGGGCTCAGCTGGTAGGCTCCACTATCTCGCCATGACTGCTGAAAATCCCACTCCTGGA
                  ************************************************************

HsCD00351586      GACCTGGCTCCGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCTGGAC
NM_182757.3       GACCTGGCTCCGGCCCCCCTCATCACTTGCAAACTCTGCCTGTGTGAGCAGTCTCTGGAC
                  ************************************************************

HsCD00351586      AAGATGACCACACTCCAGGAATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACAGTAC
NM_182757.3       AAGATGACCACACTCCAGGAATGCCAGTGCATCTTTTGCACAGCTTGCCTGAAACAGTAC
                  ************************************************************

HsCD00351586      ATGCAGCTGGCAATCCGAGAAGGATGTGGGTCTCCCATCACTTGCCCTGACATGGTGTGC
NM_182757.3       ATGCAGCTGGCAATCCGAGAAGGATGTGGGTCTCCCATCACTTGCCCTGACATGGTGTGC
                  ************************************************************

HsCD00351586      CTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTTTGGTACCTGTGGACCAGTTT
NM_182757.3       CTAAACCACGGGACCCTGCAGGAAGCTGAGATTGCCTGTTTGGTACCTGTGGACCAGTTT
                  ************************************************************

HsCD00351586      CAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTACCGAACATGG
NM_182757.3       CAACTTTATCAGAGGTTAAAATTTGAAAGAGAAGTTCATCTGGACCCCTACCGAACATGG
                  ************************************************************

HsCD00351586      TGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACAGCCT
NM_182757.3       TGTCCTGTTGCAGACTGTCAGACAGTGTGCCCTGTTGCCTCGAGTGACCCAGGACAGCCT
                  ************************************************************

HsCD00351586      GTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGCTTGG
NM_182757.3       GTGCTGGTGGAATGCCCTTCTTGCCACCTGAAATTCTGCTCGTGTTGCAAGGATGCTTGG
                  ************************************************************

HsCD00351586      CATGCAGAGGTCTCCTGTAGAGACAGTCAGCCTATTGTCCTGCCAACAGAGCACCGAGCC
NM_182757.3       CATGCAGAGGTCTCCTGTAGAGACAGTCAGCCTATTGTCCTGCCAACAGAGCACCGAGCC
                  ************************************************************

HsCD00351586      CTCTTTGGGACAGATGCAGAAGCCCCCATTAAGCAGTGCCCAGTTTGCCGGGTTTATATC
NM_182757.3       CTCTTTGGGACAGATGCAGAAGCCCCCATTAAGCAGTGCCCAGTTTGCCGGGTTTATATC
                  ************************************************************

HsCD00351586      GAACGCAATGAAGGCTGCGCTCAGATGATGTGCAAAAACTGCAAGCATACATTTTGCTGG
NM_182757.3       GAACGCAATGAAGGCTGCGCTCAGATGATGTGCAAAAACTGCAAGCATACATTTTGCTGG
                  ************************************************************

HsCD00351586      TACTGCCTCCAGAACTTGGATAATGACATTTTCCTCAGACATTATGACAAAGGGCCATGC
NM_182757.3       TACTGCCTCCAGAACTTGGATAATGACATTTTCCTCAGACATTATGACAAAGGGCCATGC
                  ************************************************************

HsCD00351586      AGGAATAAACTTGGCCACTCAAGAGCATCAGTGATGTGGAACCGAACACAGGTGGTGGGG
NM_182757.3       AGGAATAAACTTGGCCACTCAAGAGCATCAGTGATGTGGAACCGAACACAGGTGGTGGGG
                  ************************************************************

HsCD00351586      ATTCCCGTAGGCTTGGGCATCATTGCCTTGGTTACTTCACCCTTGTTACTCCTGGCCTCC
NM_182757.3       ATTCTCGTAGGCTTGGGCATCATTGCCTTGGTTACTTCACCCTTGTTACTCCTGGCCTCC
                  **** *******************************************************

HsCD00351586      CCATGTATAATCTGTTGTGTCTGCAAGTCCTGTCGGGGCAAGAAGAAAAAGCACGACCCA
NM_182757.3       CCATGTATAATCTGTTGTGTCTGCAAGTCCTGTCGGGGCAAGAAGAAAAAGCACGACCCA
                  ************************************************************

HsCD00351586      TCCACAACCTGA
NM_182757.3       TCCACAACCTAA
                  **********.*