NFIB (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NFIB
Gene Name:  nuclear factor I/B
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100067170)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351618
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1485nts
Open reading frame: 1 to 1485

ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTTCACCCATTCATCGAGGCACTT
CTTCCACATGTCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAG
TACTTTAAAAAGCATGAGAAGCGAATGTCAAAGGATGAAGAAAGAGCAGTCAAAGATGAG
CTTCTCAGTGAAAAGCCTGAAATCAAACAGAAGTGGGCATTCAGGCTCCTTGCCAAACTG
CGCAAAGATATTCGCCAGGAGTATCGAGAGGACTTTGTGCTCACCGTGACTGGCAAGAAG
CACCCGTGCTGTGTCTTATCCAATCCCGACCAGAAGGGTAAGATTAGGAGAATCGACTGC
CTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC
ATCCCCTTGGAAAGTACCGATGGAGAGCGGCTCATGAAATCCCCACATTGCACAAACCCA
GCACTTTGTGTCCAGCCACATCATATCACAGTATCAGTTAAGGAGCTTGATTTGTTTTTG
GCATACTACGTGCAGGAGCAAGATTCTGGACAATCAGGAAGTCCAAGCCACAATGATCCT
GCCAAGAATCCTCCAGGTTACCTTGAGGATAGTTTTGTAAAATCTGGAGTCTTCAATGTA
TCAGAACTTGTAAGAGTATCCAGAACGCCCATAACCCAGGGAACTGGAGTCAACTTCCCA
ATTGGAGAAATCCCAAGCCAACCATACTATCATGACATGAACTCGGGGGTCAATCTTCAG
AGGTCTCTGTCTTCTCCACCAAGCAGCAAAAGACCCAAAACTATATCCATAGATGAAAAT
ATGGAACCACGTCCTACAGGAGACTTTTACCCCTCTCCAAGTTCACCAGCTGCTGGAAGT
CGAACATGGCACGAAAGAGATCAAGATATGTCTTCTCCGACTACTATGAAGAAGCCTGAA
AAGCCATTGTTCAGCTCTGCATCTCCACAGGATTCTTCCCCAAGACTGAGCACTTTCCCC
CAGCACCACCATCCCGGAATACCTGGAGTTGCACACAGTGTCATCTCAACTCGAACTCCA
CCTCCACCTTCACCGTTGCCATTTCCAACACAAGCTATCCTTCCTCCAGCCCCATCGAGC
TACTTTTCTCATCCAACAATCAGATATCCTCCCCACCTGAATCCTCAGGATACTCTGAAG
AACTATGTACCTTCTTATGACCCATCCAGTCCACAAACCAGCCAGCCTAACGGCAGTGGT
CAAGTAGTAGGGAAAGTGCCTGGCCATTTCACTCCTGTCTTGGCACCCTCTCCCCATCCC
AGTGCAGTGCGACCTGTGACCCTGAGCATGACAGATACTAAACCCATCACTACATCCACT
GAAGCCTACACAGCCTCAGGCACATCTCAAGCCAATCGATATGTGGGACTAAGCCCAAGA
GACCCATCCTTCCTACATCAGCAACAGTCCTGGTACCTGGGCTGA

Protein sequence predicted based on nucleotide sequence

MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDE
LLSEKPEIKQKWAFRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDC
LRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFP
IGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPRPTGDFYPSPSSPAAGS
RTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
PPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQPNGSG
QVVGKVPGHFTPVLAPSPHPSAVRPVTLSMTDTKPITTSTEAYTASGTSQANRYVGLSPR
DPSFLHQQQSWYLG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable