Gene:
Gene Symbol:
IGSF3
Gene Name:
immunoglobulin superfamily, member 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100067125)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351628
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGTGCTTTTTCCCGGTGCTGAGCTGTCTGGCTGTGCTGGGTGTGGTGTCAGCACAG
CGGCAGGCCACCGTTCAGGAAGGACCCTTGTACCGCACGGAGGGCTCCCACATCACTATC
TGGTGCAATGTGAGTGGCTACCAGGGACCTTCTGAGCAGAATTTCCAGTGGTCCATTTAC
CTGCCTTCGTCGCCAGAGCGAGAGGTGCGGATCGTCAGCACCATGGACTCTTCCTTCCCC
TATGCCATCTACACCCAGCGCGTCCGCGGAGGGAAGATCTTCATAGAAAGAGTCCAGGGG
AACTCAACCCTATTGCACATCACAGATCTTCAGGCCCGGGATGCCGGGGAGTATGAATGC
CACACACCCAGCACTGATAAGCAATACTTTGGGAGTTACAGTGCAAAGATGAACCTAGTG
GTGATCCCAGACTCCCTGCAGACCACTGCCATGCCCCAGACTCTGCACAGAGTGGAGCAG
GACCCGCTGGAGCTCACTTGTGAGGTGGCCTCAGAGACCATTCAGCACAGCCACCTGTCT
GTGGCCTGGCTCCGGCAGAAAGTTGGCGAGAAGCCCGTGGAGGTCATCTCCCTGAGCCGA
GATTTCATGCTTCACTCCAGCAGCGAATATGCCCAGAGGCAGAGCCTGGGGGAGGTGCGG
CTGGACAAGCTGGGGAGGACCACCTTCCGCCTCACCATCTTCCACCTGCAGCCTTCTGAC
CAGGGCGAATTCTACTGCGAGGCCGCCGAGTGGATCCAGGATCCGGATGGGTCGTGGTAT
GCTATGACCCGAAAGCGTTCCGAGGGAGCCGTGGTCAACGTCCAGCCAACTGACAAAGAA
TTCACTGTTCGGCTGGAGACAGAGAAGCGGCTGCACACGGTGGGCGAGCCGGTGGAGTTC
AGATGCATCCTGGAGGCTCAGAATGTTCCCGACCGTTACTTTGCTGTCTCCTGGGCCTTC
AACAGCTCGCTCATCGCCACCATGGGTCCTAACGCTGTGCCTGTCCTCAACAGCGAATTT
GCTCACCGGGAAGCCAGGGGACAGCTTAAGGTGGCCAAAGAGAGCGACAGTGTCTTTGTG
CTGAAGATCTACCACCTCCGCCAGGAAGATAGCGGGAAATACAACTGCCGGGTGACTGAG
CGAGAGAAAACCGTGACCGGGGAATTCATTGATAAGGAGAGCAAGCGTCCCAAGAACATC
CCCATCATAGTCCTCCCCCTCAAGAGCAGCATCTCCGTGGAGGTGGCCAGCAATGCCAGC
GTCATCCTTGAGGGCGAGGACCTGCGCTTCTCCTGCAGTGTCCGCACGGCAGGCAGGCCG
CAGGGTCGCTTCTCTGTCATCTGGCAGCTTGTGGACAGGCAGAACCGCCGCAGCAATATC
ATGTGGCTAGACCGGGATGGCACCGTGCAGCCAGGCTCGTCCTACTGGGAGCGCAGCAGC
TTTGGGGGCGTCCAGATGGAGCAGGTGCAGCCCAACTCGTTCAGCCTGGGCATCTTCAAC
AGCAGGAAGGAGGACGAGGGCCAGTATGAATGCCATGTGACTGAATGGGTGCGGGCAGTG
GATGGCGAGTGGCAGATTGTTGGGGAGCGCCGGGCCAGCACTCCCATCTCCATCACAGCT
CTTGAAATGGGCTTCGCAGTCACAGCCATCTCCCGGACACCGGGGGTGACCTACAGCGAC
TCCTTTGACTTGCAGTGTATCATCAAACCCCACTACCCTGCCTGGGTCCCCGTGTCGGTG
ACATGGCGGTTCCAGCCGGTGGGCACGGTGGAGTTCCATGACTTGGTGACCTTCACCCGG
GACGGAGGGGTCCAGTGGGGGGACAGGTCCTCCAGCTTCCGAACCCGAACTGCCATCGAG
AAGGCTGAGTCCAGCAACAACGTCCGCCTAAGCATCAGCCGAGCCAGTGACACGGAAGCA
GGCAAGTACCAGTGTGTGGCAGAGCTGTGGCGGAAGAACTACAACAACACCTGGACGCGA
CTGGCGGAGAGGACCTCCAACCTGCTGGAGATCAGGGTGCTGCAGCCAGTGACAAAGCTG
CAGGTGAGCAAATCGAAGAGGACCCTCACCCTGGTGGAAAACAAGCCCATTCAGTTGAAC
TGCTCAGTCAAGTCTCAGACTAGCCAGAACTCCCACTTTGCGGTGCTCTGGTATGTCCAC
AAGCCCTCGGATGCCGATGGCAAGCTTATCCTGAAGACCACCCACAACTCCGCCTTTGAA
TACGGTACTTACGCCGAGGAGGAGGGCCTGAGAGCCAGGCTCCAGTTTGAGAGGCATGTG
TCGGGGGGCCTGTTCAGCCTCACCGTCCAGAGAGCCGAGGTCAGCGACAGCGGCAGCTAC
TACTGCCACGTGGAGGAGTGGCTGCTGAGCCCCAACTACGCCTGGTACAAGCTGGCAGAG
GAGGTTTCTGGGCGCACAGAAGTCACTGTGAAACAGCCAGACAGCCGCCTGAGGCTCAGC
CAAGCCCAGGGGAACCTGTCGGTTCTGGAGACCCGGCAGGTACAGCTGGAGTGTGTGGTT
CTCAACCGCACCAGCATAACCTCCCAGCTCATGGTGGAATGGTTTGTATGGAAGCCCAAC
CACCCTGAGCGGGAGACTGTGGCCCGCTTGAGCCGTGACGCCACCTTCCACTATGGAGAG
CAGGCAGCCAAGAACAATCTGAAGGGGCGGCTGCATTTGGAGAGTCCTTCCCCCGGCGTG
TACCGTCTCTTCATCCAGAACGTGGCTGTGCAGGACAGCGGGACCTACAGCTGCCATGTG
GAGGAGTGGCTGCCCAGCCCCAGTGGCATGTGGTATAAGCGGGCAGAGGACACCGCTGGG
CAGACAGCTCTGACAGTCATGCGACCAGATGCTTCCCTGCAGGTGGACACAGTGGTCCCC
AATGCCACGGTCTCTGAGAAGGCAGCTTTCCAGCTGGACTGTAGCATCGTGTCCCGCTCC
AGCCAGGACTCCCGCTTCGCTGTGGCCTGGTATTCCCTGAGGACTAAAGCTGGGGGGAAA
AGGAGCAGCCCTGGCCTGGAAGAACAGGAAGAGGAAAGGGAGGAGGAGGAGGAGGAGGAG
GAGGACGACGACGACGACGACCCAACAGAGCGGACGGCCCTGCTGAGCGTGGGCCCAGAT
GCTGTCTTTGGCCCAGAGGGCAGTCCTTGGGAGGGCAGGCTTCGCTTCCAGAGGCTCTCC
CCGGTGCTCTACCGGCTCACAGTGCTGCAGGCAAGCCCCCAAGATACAGGCAATTACTCC
TGCCATGTGGAGGAGTGGCTGCCCAGCCCTCAGAAGGAATGGTACCGGCTGACGGAGGAG
GAGTCAGCCCCCATCGGCATCCGTGTTCTAGATACAAGTCCCACCCTCCAGTCCATCATC
TGCTCCAACGACGCACTCTTCTACTTCGTCTTCTTCTACCCTTTCCCCATCTTTGGCATT
CTTATCATCACCATCCTTCTGGTGCGTTTCAAGAGCCGGAACTCCAGCAAGAACTCTGAT
GGGAAGAATGGGGTGCCTCTGCTGTGGATCAAAGAGCCACACCTCAACTACTCCCCTACT
TGCCTGGAGCCCCCTGTTCTCAGTACCCATCCAGGGGCCATAGACTGA
Protein sequence predicted based on nucleotide sequence
MKCFFPVLSCLAVLGVVSAQRQATVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIY LPSSPEREVRIVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYEC HTPSTDKQYFGSYSAKMNLVVIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLS VAWLRQKVGEKPVEVISLSRDFMLHSSSEYAQRQSLGEVRLDKLGRTTFRLTIFHLQPSD QGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKEFTVRLETEKRLHTVGEPVEF RCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLKVAKESDSVFV LKIYHLRQEDSGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPLKSSISVEVASNAS VILEGEDLRFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQPGSSYWERSS FGGVQMEQVQPNSFSLGIFNSRKEDEGQYECHVTEWVRAVDGEWQIVGERRASTPISITA LEMGFAVTAISRTPGVTYSDSFDLQCIIKPHYPAWVPVSVTWRFQPVGTVEFHDLVTFTR DGGVQWGDRSSSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRKNYNNTWTR LAERTSNLLEIRVLQPVTKLQVSKSKRTLTLVENKPIQLNCSVKSQTSQNSHFAVLWYVH KPSDADGKLILKTTHNSAFEYGTYAEEEGLRARLQFERHVSGGLFSLTVQRAEVSDSGSY YCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLRLSQAQGNLSVLETRQVQLECVV LNRTSITSQLMVEWFVWKPNHPERETVARLSRDATFHYGEQAAKNNLKGRLHLESPSPGV YRLFIQNVAVQDSGTYSCHVEEWLPSPSGMWYKRAEDTAGQTALTVMRPDASLQVDTVVP NATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRSSPGLEEQEEEREEEEEEE EDDDDDDPTERTALLSVGPDAVFGPEGSPWEGRLRFQRLSPVLYRLTVLQASPQDTGNYS CHVEEWLPSPQKEWYRLTEEESAPIGIRVLDTSPTLQSIICSNDALFYFVFFYPFPIFGI LIITILLVRFKSRNSSKNSDGKNGVPLLWIKEPHLNYSPTCLEPPVLSTHPGAID*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR221
Synonyms:
pDONR221, pENTR221-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.
Size (bp):
2574
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin of replication
1905
4
primer site
M13 F
M13 forward priming site
846
865
primer site
M13 Rev
M13 reverse primer site
846
865
recombination site
attL1
attL1 recombination site
575
675
recombination site
attL2
attL2 recombination site
805
706
recombination site
rrnBT1
rrnB T1 transcription termination sequence
434
477
recombination site
rrnBT2
rrnB T2 transcription termination sequence
275
302
selectable marker
KanR
kanamycin resistance gene
975
1784
