Gene:
Gene Symbol:
MYO1B
Gene Name:
myosin IB
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100067136)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351629
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCAAAATGGAGGTGAAAACCTCACTTCTGGACAATATGATTGGAGTTGGGGATATG
GTTCTTTTAGAACCTCTCAATGAGGAGACCTTCATCAACAACCTCAAGAAGCGCTTTGAC
CACAGTGAAATATACACTTACATTGGAAGTGTGGTTATATCTGTTAACCCATATCGGTCT
TTACCCATTTATTCACCAGAGAAAGTGGAAGAATACAGGAACAGAAATTTTTATGAACTG
AGCCCTCACATCTTTGCCCTTTCGGATGAAGCATACAGATCCCTACGAGATCAAGATAAG
GACCAATGTATTCTCATTACTGGGGAAAGTGGAGCAGGAAAAACAGAGGCCAGTAAGCTT
GTCATGTCCTATGTGGCAGCTGTTTGTGGAAAAGGAGCAGAAGTTAATCAAGTTAAAGAA
CAGCTTTTACAGTCCAACCCGGTCCTGGAAGCTTTTGGAAATGCCAAAACTGTAAGGAAT
GACAACTCCTCTAGATTTGGCAAATATATGGATATTGAATTTGACTTTAAAGGCGATCCA
CTAGGAGGAGTAATAAGTAACTATCTTTTAGAGAAATCTCGGGTTGTTAAACAGCCAAGA
GGTGAAAGAAACTTCCATGTGTTCTATCAGCTGCTCTCTGGTGCCTCTGAAGAGCTCCTC
AATAAACTTAAGCTTGAGAGGGATTTCAGCAGGTATAACTACCTGAGTCTGGATTCGGCC
AAAGTGAATGGAGTGGATGATGCAGCAAATTTTAGAACCGTGCGGAATGCCATGCAGATT
GTGGGCTTTATGGATCATGAAGCTGAGTCTGTCTTGGCGGTGGTGGCAGCAGTGTTGAAA
CTGGGGAACATTGAGTTCAAGCCCGAATCTCGAGTGAATGGTCTAGATGAAAGCAAAATC
AAAGATAAAAATGAGTTAAAAGAAATTTGTGAATTGACCGGCATTGATCAATCAGTTCTA
GAACGAGCATTCAGTTTCCGAACAGTTGAGGCCAAACAGGAGAAAGTTTCAACTACACTG
AATGTGGCTCAGGCTTATTATGCCCGTGATGCTCTGGCTAAAAACCTCTACAGCAGGTTG
TTTTCATGGTTGGTAAATCGAATCAATGAAAGCATTAAGGCACAAACAAAAGTGAGAAAG
AAGGTCATGGGTGTTCTGGACATTTATGGCTTTGAGATTTTCGAGGACAACAGCTTTGAG
CAGTTCATTATTAATTATTGTAACGAAAAGCTGCAACAAATCTTCATTGAACTTACTCTT
AAAGAAGAGCAGGAGGAGTATATACGGGAGGATATAGAATGGACTCACATTGACTACTTC
AATAATGCTATCATTTGTGACCTAATAGAAAATAACACAAATGGAATCCTGGCCATGCTG
GATGAAGAGTGCCTCAGACCTGGCACAGTCACTGATGAGACCTTCTTAGAAAAGCTGAAC
CAAGTATGTGCCACCCACCAGCATTTTGAAAGCAGGATGAGCAAGTGCTCTCGGTTCCTC
AATGACACGTCTCTGCCTCACAGCTGCTTCAGGATCCAGCATTATGCTGGAAAGGTGCTG
TACCAGGTGGAAGGATTCGTTGACAAAAACAATGACCTTCTCTATCGAGACCTGTCCCAA
GCCATGTGGAAGGCCAGCCATGCCCTCATCAAGTCTTTGTTCCCCGAAGGGAATCCCGCC
AAGATCAACCTGAAAAGGCCTCCTACAGCAGGCTCACAGTTCAAGGCATCCGTGGCCACT
CTGATGAAAAACCTACAGACCAAGAACCCAAACTATATTAGGTGTATCAAACCGAATGAT
AAAAAAGCAGCACACATCTTCAACGAGGCTCTAGTGTGTCATCAGATCAGGTACCTGGGG
CTTTTGGAGAACGTCCGAGTGCGGAGGGCAGGCTACGCCTTCAGGCAGGCCTATGAACCT
TGCCTAGAAAGATACAAAATGCTTTGTAAACAAACATGGCCTCATTGGAAAGGACCAGCC
AGGTCTGGTGTGGAGGTCCTATTTAATGAATTAGAAATTCCCGTGGAAGAATACTCCTTT
GGTAGATCAAAGATATTCATCCGAAACCCAAGAACATTATTCAAATTAGAAGACCTGAGG
AAGCAACGCCTGGAGGACTTGGCCACTCTCATTCAGAAGATATATCGGGGGTGGAAATGC
CGCACACACTTCCTGCTAATGAAAAAAAGCCAAATTGTGATTGCCGCCTGGTACAGGAGA
TATGCGCAACAAAAGAGGTACCAGCAGACAAAGAGTTCCGCCTTAGTAATTCAGTCTTAT
ATCCGGGGTTGGAAGGCTCGAAAAATTCTGCGGGAACTGAAGCATCAAAAGCGCTGTAAG
GAAGCAGTCACGACCATTGCTGCATATTGGCATGGGACCCAGGCACGAAGGGAACTGAGA
CGGCTGAAGGAGGAGGCTAGGAATAAACATGCTATTGCAGTTATTTGGGCTTACTGGCTT
GGATCTAAGGCTCGAAGGGAATTGAAACGCTTGAAGGAGGAGGCTAGGCGTAAGCATGCA
GTTGCTGTCATTTGGGCTTACTGGCTTGGACTGAAGGTACGTAGAGAATACAGGAAATTC
TTCAGAGCCAATGCTGGAAAGAAAATCTATGAGTTTACGCTTCAGAGAATTGTGCAAAAA
TACTTCTTGGAAATGAAAAATAAGATGCCTTCCTTATCTCCAATAGACAAGAATTGGCCC
TCAAGACCTTACTTATTCTTGGATTCTACTCACAAGGAGCTAAAAAGGATTTTCCACTTG
TGGAGGTGTAAAAAATACAGGGACCAATTCACAGACCAGCAGAAACTTATTTATGAAGAG
AAACTAGAAGCCAGTGAACTCTTCAAAGACAAGAAGGCTTTATACCCATCTAGTGTTGGG
CAACCATTCCAAGGGGCTTACCTGGAAATCAACAAGAACCCCAAGTATAAGAAACTCAAA
GATGCCATTGAAGAAAAGATCATCATTGCTGAAGTCGTGAACAAAATTAACCGTGCTAAT
GGGAAGAGTACATCTCGGATTTTCCTCTTAACAAACAATAATCTCCTTCTTGCTGACCAA
AAGTCTGGACAAATCAAGTCAGAGGTTCCACTGGTGGATGTGACCAAGGTATCAATGAGC
TCACAAAATGATGGCTTCTTCGCCGTCCACCTCAAAGAGGGCTCAGAAGCAGCTAGTAAA
GGAGACTTTCTCTTCAGCAGTGATCACCTGATTGAAATGGCCACCAAGCTCTATCGCACA
ACTCTCAGCCAAACCAAACAGAAGCTCAATATTGAGATTTCCGATGAGTTCCTGGTACAG
TTCAGACAGGACAAAGTATGTGTGAAGTTTATTCAGGGAAACCAGAAAAATGGGAGTGTC
CCAACATGTAAACGAAAAAACAACCGTCTCCTTGAAGTTGCTGTCCCT
Protein sequence predicted based on nucleotide sequence
MAKMEVKTSLLDNMIGVGDMVLLEPLNEETFINNLKKRFDHSEIYTYIGSVVISVNPYRS LPIYSPEKVEEYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKL VMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDP LGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLNKLKLERDFSRYNYLSLDSA KVNGVDDAANFRTVRNAMQIVGFMDHEAESVLAVVAAVLKLGNIEFKPESRVNGLDESKI KDKNELKEICELTGIDQSVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRL FSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTL KEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLN QVCATHQHFESRMSKCSRFLNDTSLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQ AMWKASHALIKSLFPEGNPAKINLKRPPTAGSQFKASVATLMKNLQTKNPNYIRCIKPND KKAAHIFNEALVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPA RSGVEVLFNELEIPVEEYSFGRSKIFIRNPRTLFKLEDLRKQRLEDLATLIQKIYRGWKC RTHFLLMKKSQIVIAAWYRRYAQQKRYQQTKSSALVIQSYIRGWKARKILRELKHQKRCK EAVTTIAAYWHGTQARRELRRLKEEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHA VAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYFLEMKNKMPSLSPIDKNWP SRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVG QPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQ KSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRT TLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR221
Synonyms:
pDONR221, pENTR221-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.
Size (bp):
2574
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin of replication
1905
4
primer site
M13 F
M13 forward priming site
846
865
primer site
M13 Rev
M13 reverse primer site
846
865
recombination site
attL1
attL1 recombination site
575
675
recombination site
attL2
attL2 recombination site
805
706
recombination site
rrnBT1
rrnB T1 transcription termination sequence
434
477
recombination site
rrnBT2
rrnB T2 transcription termination sequence
275
302
selectable marker
KanR
kanamycin resistance gene
975
1784
