Gene:
Gene Symbol:
NLRC4
Gene Name:
NLR family, CARD domain containing 4
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100067162)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351647
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATTTCATAAAGGACAATAGCCGAGCCCTTATTCAAAGAATGGGAATGACTGTTATA
AAGCAAATCACAGATGACCTATTTGTATGGAATGTTCTGAATCGCGAAGAAGTAAACATC
ATTTGCTGCGAGAAGGTGGAGCAGGATGCTGCTAGAGGGATCATTCACATGATTTTGAAA
AAGGGTTCAGAGTCCTGTAACCTCTTTCTTAAATCCCTTAAGGAGTGGAACTATCCTCTA
TTTCAGGACTTGAATGGACAAAGTCTTTTTCATCAGACATCAGAAGGAGACTTGGACGAT
TTGGCTCAGGATTTAAAGGACTTGTACCATACCCCATCTTTTCTGAACTTTTATCCCCTT
GGTGAAGATATTGACATTATTTTTAACTTGAAAAGCACCTTCACAGAACCTGTCCTGTGG
AGGAAGGACCAACACCATCACCGCGTGGAGCAGCTGACCCTGAATGGCCTCCTGCAGGCT
CTTCAGAGCCCCTGCATCATTGAAGGGGAATCTGGCAAAGGCAAGTCCACTCTGCTGCAG
CGAATTGCCATGCTCTGGGGCTCCGGAAAGTGCAAGGCTCTGACCAAGTTCAAATTCGTC
TTCTTCCTCCGTCTCAGCAGGGCCCAGGGTGGACTTTTTGAAACCCTCTGTGATCAACTC
CTGGATATACCTGGCACAATCAGGAAGCAGACATTCATGGCCATGCTGCTGAAGCTGCGG
CAGAGGGTTCTTTTCCTTCTTGATGGCTACAATGAATTCAAGCCCCAGAACTGCCCAGAA
ATCGAAGCCCTGATAAAGGAAAACCACCGCTTCAAGAACATGGTCATCGTCACCACTACC
ACTGAGTGCCTGAGGCACATACGGCAGTTTGGTGCCCTGACTGCTGAGGTGGGGGATATG
ACAGAAGACAGCGCCCAGGCTCTCATCCGAGAAGTGCTGATCAAGGAGCTTGCTGAAGGC
TTGTTGCTCCAAATTCAGAAATCCAGGTGCTTGAGGAATCTCATGAAGACCCCTCTCTTT
GTGGTCATCACTTGTGCAATCCAGATGGGTGAAAGTGAGTTCCACTCTCACACACAAACA
ACGCTGTTCCATACCTTCTATGATCTGTTGATACAGAAAAACAAACACAAACATAAAGGT
GTGGCTGCAAGTGACTTCATTCGGAGCCTGGACCACTGTGGAGACCTAGCTCTGGAGGGT
GTGTTCTCCCACAAGTTTGATTTCGAACTGCAGGATGTGTCCAGCGTGAATGAGGATGTC
CTGCTGACAACTGGGCTCCTCTGTAAATATACAGCTCAAAGGTTCAAGCCAAAGTATAAA
TTCTTTCACAAGTCATTCCAGGAGTACACAGCAGGACGAAGACTCAGCAGTTTATTGACG
TCTCATGAGCCAGAGGAGGTGACCAAGGGGAATGGTTACTTGCAGAAAATGGTTTCCATT
TCGGACATTACATCCACTTATAGCAGCCTGCTCCGGTACACCTGTGGGTCATCTGTGGAA
GCCACCAGGGCTGTTATGAAGCACCTCGCAGCAGTGTATCAACACGGCTGCCTTCTCGGA
CTTTCCATCGCCAAGAGGCCTCTCTGGAGACAGGAATCTTTGCAAAGTGTGAAAAACACC
ACTGAGCAAGAAATTCTGAAAGCCATAAACATCAATTCCTTTGTAGAGTGTGGCATCCAT
TTATATCAAGAGAGTACATCCAAATCAGCCCTGAGCCAAGAATTTGAAGCTTTCTTTCAA
GGTAAAAGCTTATATATCAACTCAGGGAACATCCCCGATTACTTATTTGACTTCTTTGAA
CATTTGCCCAATTGTGCAAGTGCCCTGGACTTCATTAAACTGGACTTTTATGGGGGAGCT
ATGGCTTCATGGGAAAAGGCTGCAGAAGACACAGGTGGAATCCACATGGAAGAGGCCCCA
GAAACCTACATTCCCAGCAGGGCTGTATCTTTGTTCTTCAACTGGAAGCAGGAATTCAGG
ACTCTGGAGGTCACACTCCGGGATTTCAGCAAGTTGAATAAGCAAGATATCAGATATCTG
GGGAAAATATTCAGCTCTGCCACAAGCCTCAGGCTGCAAATAAAGAGATGTGCTGGTGTG
GCTGGAAGCCTCAGTTTGGTCCTCAGCACCTGTAAGAACATTTATTCTCTCATGGTGGAA
GCCAGTCCCCTCACCATAGAAGATGAGAGGCACATCACATCTGTAACAAACCTGAAAACC
TTGAGTATTCATGACCTACAGAATCAACGGCTGCCGGGTGGTCTGACTGACAGCTTGGGT
AACTTGAAGAACCTTACAAAGCTCATAATGGATAACATAAAGATGAATGAAGAAGATGCT
ATAAAACTAGCTGAAGGCCTGAAAAACCTGAAGAAGATGTGTTTATTTCATTTGACCCAC
TTGTCTGACATTGGAGAGGGAATGGATTACATAGTCAAGTCTCTGTCAAGTGAACCCTGT
GACCTTGAAGAAATTCAATTAGTCTCCTGCTGCTTGTCTGCAAATGCAGTGAAAATCCTA
GCTCAGAATCTTCACAATTTGGTCAAACTGAGCATTCTTGATTTATCAGAAAATTACCTG
GAAAAAGATGGAAATGAAGCTCTTCATGAACTGATCGACAGGATGAACGTGCTAGAACAG
CTCACCGCACTGATGCTGCCCTGGGGCTGTGACGTGCAAGGCAGCCTGAGCAGCCTGTTG
AAACATTTGGAGGAGGTCCCACAACTCGTCAAGCTTGGGTTGAAAAACTGGAGACTCACA
GATACAGAGATTAGAATTTTAGGTGCATTTTTTGGAAAGAACCCTCTGAAAAACTTCCAG
CAGTTGAATTTGGCGGGAAATCGTGTGAGCAGTGATGGATGGCTTGCCTTCATGGGTGTA
TTTGAGAATCTTAAGCAATTAGTGTTTTTTGACTTTAGTACTAAAGAATTTCTACCTGAT
CCAGCATTAGTCAGAAAACTTAGCCAAGTGTTATCCAAGTTAACTTTTCTGCAAGAAGCT
AGGCTTGTTGGGTGGCAATTTGATGATGATGATCTCAGTGTTATTACAGGTGCTTTTAAA
CTAGTAACTGCTTGA
Protein sequence predicted based on nucleotide sequence
MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILK KGSESCNLFLKSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPL GEDIDIIFNLKSTFTEPVLWRKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQ RIAMLWGSGKCKALTKFKFVFFLRLSRAQGGLFETLCDQLLDIPGTIRKQTFMAMLLKLR QRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRQFGALTAEVGDM TEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGESEFHSHTQT TLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDV LLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSI SDITSTYSSLLRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNT TEQEILKAININSFVECGIHLYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFE HLPNCASALDFIKLDFYGGAMASWEKAAEDTGGIHMEEAPETYIPSRAVSLFFNWKQEFR TLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGVAGSLSLVLSTCKNIYSLMVE ASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIMDNIKMNEEDA IKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKIL AQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLL KHLEEVPQLVKLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGV FENLKQLVFFDFSTKEFLPDPALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFK LVTA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR221
Synonyms:
pDONR221, pENTR221-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.
Size (bp):
2574
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin of replication
1905
4
primer site
M13 F
M13 forward priming site
846
865
primer site
M13 Rev
M13 reverse primer site
846
865
recombination site
attL1
attL1 recombination site
575
675
recombination site
attL2
attL2 recombination site
805
706
recombination site
rrnBT1
rrnB T1 transcription termination sequence
434
477
recombination site
rrnBT2
rrnB T2 transcription termination sequence
275
302
selectable marker
KanR
kanamycin resistance gene
975
1784
