MAP4K3 (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MAP4K3
Gene Name:  mitogen-activated protein kinase kinase kinase kinase 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100068474)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351685
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2619nts
Open reading frame: 1 to 2619

ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAG
CGCATCGGCAGCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAA
TTAGCAGCAATTAAAGTAATAAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAA
GAAATTATCATGATGAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTAT
CTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTACAGGAT
ATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAGCAGAGAAACA
CTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAG
ATAACAGCTACAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCA
GAAGTTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTG
GGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTTTGACTTACACCCAATG
AGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTCCTAAACTAAAGGATAAAATG
AAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAA
AGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTC
GATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGA
AACGTGAGAGAAGAAAAAACACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCA
CCCTTAAGAAAGGAGACAGAACCACATCATGAACTTGATCTGCAACTGGAATATGGACAA
GGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAA
GAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGATGAA
TCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCT
ATCTTCATACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGA
TGTCCCATGTCAGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCC
AGATTACCCCCACACAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAAT
GGTGAACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAACCTTTCA
AGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCCTCCAACACCTAAA
GTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCACTGT
GCATCATCATGGATAAACCCAGATACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAA
GGGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCT
CGAAGGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCT
TCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGCAAGACAAATGCAAAAG
TTACCTGTTGCTATTCCAGCACACAAACTCCCTGACAGAATACTGCCAAGGAAATTTTCT
GTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCT
TACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAATGG
GTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCA
CTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGT
GTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCT
ACCTCTTCATGGTTTACAGAATCAGATACCCCACAGACAAATGTTACTCATGTAACCCAA
CTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTCCAA
GGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAGATTGAA
TCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGTAGA
AGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTG
CTTGGATCTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAAT
AGCAATTTGTACATCCTGGCGGGTCATGAAAACAGTTAC

Protein sequence predicted based on nucleotide sequence

MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQ
EIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRET
LQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAP
EVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKM
KWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF
DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQ
GHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKS
IFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLN
GERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHC
ASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKA
SQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNP
YTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVG
VSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQ
GRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRL
LGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable