DNASU Plasmid | CHST7 (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CHST7

Gene Name: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100068603)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351757

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1461nts

Open reading frame: 1 to 1461

Reference Sequence Alignment

ATGAAGGGCCGGCGGCGGCGACGCCGAGAGTACTGCAAGTTCGCGCTGCTGTTGGTGCTG
TACACGCTGGTGCTGTTGCTCGTCCCCTCCGTATTGGACGGCGGCCGCGACGGGGACAAG
GGCGCCGAGCACTGCCCCGGCCTGCAGCGCAGCCTGGGAGTGTGGAGCCTGGAGGCGGCG
GCGGCCGGCGAACGCGAGCAGGGAGCGGAGGCGCGGGCCGCCGAGGAAGGGGGCGCGAAC
CAGTCTCCTCGGTTCCCAAGCAACCTCAGCGGCGCTGTCGGGGAGGCAGTGTCTCGCGAG
AAGCAGCACATCTACGTGCATGCCACCTGGCGCACCGGCTCGTCCTTCCTGGGCGAACTC
TTTAACCAGCACCCGGACGTTTTCTACTTGTATGAGCCCATGTGGCATCTATGGCAGGCG
CTGTATCCGGGCGACGCCGAGAGCTTGCAGGGCGCGCTGCGCGACATGCTGCGTTCGCTC
TTCCGCTGCGACTTCTCCGTGCTGCGGCTGTACGCGCCGCCGGGGGACCCCGCTGCGCGC
GCCCCGGACACGGCCAATCTTACCACGGCCGCCCTCTTCCGCTGGCGGACTAACAAGGTC
ATCTGCTCGCCGCCACTGTGTCCTGGCGCACCCCGTGCCCGGGCCGAGGTGGGCCTCGTC
GAGGACACCGCCTGCGAGCGCAGCTGCCCACCCGTGGCGATACGCGCCCTGGAGGCCGAG
TGCCGAAAGTACCCGGTGGTGGTCATCAAGGACGTGCGCCTGCTCGATCTGGGCGTGCTG
GTGCCCCTGTTGCGTGATCCAGGCCTCAACCTGAAGGTGGTGCAGCTTTTCCGCGACCCG
AGGGCGGTGCACAACTCGCGCCTCAAGTCTAGGCAGGGACTGCTGCGCGAGAGCATCCAG
GTGCTGCGCACCCGCCAGAGGGGCGACCGCTTCCACCGTGTGCTGCTGGCGCACGGCGTG
GGTGCTCGCCCCGGGGGCCAGTCTCGCGCGCTGCCCGCCGCGCCGCGCGCCGATTTCTTC
CTGACCGGTGCGCTCGAGGTGATCTGCGAAGCCTGGCTGCGCGATCTGCTTTTCGCGCGC
GGCGCGCCCGCCTGGCTGCGGCGCCGCTACCTGAGGCTGCGCTATGAGGACCTGGTGCGG
CAGCCACGCGCCCAGCTGCGCCGCCTGCTGCGCTTCTCCGGGCTACGCGCGCTCGCAGCG
CTCGATGCCTTCGCGCTCAACATGACTCGCGGCGCGGCCTACGGCGCCGACCGGCCCTTC
CACCTGTCAGCGCGCGACGCCCGGGAGGCGGTGCACGCCTGGCGCGAGCGCCTGAGCCGA
GAGCAGGTGCGCCAGGTGGAGGCCGCCTGCGCTCCAGCCATGCGTCTGCTCGCCTACCCT
CGCAGCGGAGAGGAGGGCGACGCGGAGCAGCCCAGGGAAGGGGAGACGCCGCTGGAGATG
GATGCCGACGGCGCCACGTGA

Protein sequence predicted based on nucleotide sequence

MKGRRRRRREYCKFALLLVLYTLVLLLVPSVLDGGRDGDKGAEHCPGLQRSLGVWSLEAA
AAGEREQGAEARAAEEGGANQSPRFPSNLSGAVGEAVSREKQHIYVHATWRTGSSFLGEL
FNQHPDVFYLYEPMWHLWQALYPGDAESLQGALRDMLRSLFRCDFSVLRLYAPPGDPAAR
APDTANLTTAALFRWRTNKVICSPPLCPGAPRARAEVGLVEDTACERSCPPVAIRALEAE
CRKYPVVVIKDVRLLDLGVLVPLLRDPGLNLKVVQLFRDPRAVHNSRLKSRQGLLRESIQ
VLRTRQRGDRFHRVLLAHGVGARPGGQSRALPAAPRADFFLTGALEVICEAWLRDLLFAR
GAPAWLRRRYLRLRYEDLVRQPRAQLRRLLRFSGLRALAALDAFALNMTRGAAYGADRPF
HLSARDAREAVHAWRERLSREQVRQVEAACAPAMRLLAYPRSGEEGDAEQPREGETPLEM
DADGAT*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

CHST7

Symbol Nomenclature :

CHST7

SYNONYM :

C6ST-2; GST-5

Designation :

Full Nomenclature :

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7

GENEID :

56548

Locus Tag :

RP1-71L16.8

GenBank Accession :

564364

HGNC :

13817

MIM :

300375

Vega :

OTTHUMG00000021423

Target GenBank:

GQ129387

Reference Sequence Alignment

  

HsCD00351757      ATGAAGGGCCGGCGGCGGCGACGCCGAGAGTACTGCAAGTTCGCGCTGCTGTTGGTGCTG
NM_019886.3       ATGAAGGGCCGGCGGCGGCGACGCCGAGAGTACTGCAAGTTCGCGCTGCTGTTGGTGCTG
                  ************************************************************

HsCD00351757      TACACGCTGGTGCTGTTGCTCGTCCCCTCCGTATTGGACGGCGGCCGCGACGGGGACAAG
NM_019886.3       TACACGCTGGTGCTGTTGCTCGTCCCCTCCGTATTGGACGGCGGCCGCGACGGGGACAAG
                  ************************************************************

HsCD00351757      GGCGCCGAGCACTGCCCCGGCCTGCAGCGCAGCCTGGGAGTGTGGAGCCTGGAGGCGGCG
NM_019886.3       GGCGCCGAGCACTGCCCCGGCCTGCAGCGCAGCCTGGGAGTGTGGAGCCTGGAGGCGGCG
                  ************************************************************

HsCD00351757      GCGGCCGGCGAACGCGAGCAGGGAGCGGAGGCGCGGGCCGCCGAGGAAGGGGGCGCGAAC
NM_019886.3       GCGGCCGGCGAACGCGAGCAGGGAGCGGAGGCGCGGGCCGCCGAGGAAGGGGGCGCGAAC
                  ************************************************************

HsCD00351757      CAGTCTCCTCGGTTCCCAAGCAACCTCAGCGGCGCTGTCGGGGAGGCAGTGTCTCGCGAG
NM_019886.3       CAGTCTCCTCGGTTCCCAAGCAACCTCAGCGGCGCTGTCGGGGAGGCAGTGTCTCGCGAG
                  ************************************************************

HsCD00351757      AAGCAGCACATCTACGTGCATGCCACCTGGCGCACCGGCTCGTCCTTCCTGGGCGAACTC
NM_019886.3       AAGCAGCACATCTACGTGCATGCCACCTGGCGCACCGGCTCGTCCTTCCTGGGCGAACTC
                  ************************************************************

HsCD00351757      TTTAACCAGCACCCGGACGTTTTCTACTTGTATGAGCCCATGTGGCATCTATGGCAGGCG
NM_019886.3       TTTAACCAGCACCCGGACGTTTTCTACTTGTATGAGCCCATGTGGCATCTATGGCAGGCG
                  ************************************************************

HsCD00351757      CTGTATCCGGGCGACGCCGAGAGCTTGCAGGGCGCGCTGCGCGACATGCTGCGTTCGCTC
NM_019886.3       CTGTATCCGGGCGACGCCGAGAGCTTGCAGGGCGCGCTGCGCGACATGCTGCGTTCGCTC
                  ************************************************************

HsCD00351757      TTCCGCTGCGACTTCTCCGTGCTGCGGCTGTACGCGCCGCCGGGGGACCCCGCTGCGCGC
NM_019886.3       TTCCGCTGCGACTTCTCCGTGCTGCGGCTGTACGCGCCGCCGGGGGACCCCGCTGCGCGC
                  ************************************************************

HsCD00351757      GCCCCGGACACGGCCAATCTTACCACGGCCGCCCTCTTCCGCTGGCGGACTAACAAGGTC
NM_019886.3       GCCCCGGACACGGCCAATCTTACCACGGCCGCCCTCTTCCGCTGGCGGACTAACAAGGTC
                  ************************************************************

HsCD00351757      ATCTGCTCGCCGCCACTGTGTCCTGGCGCACCCCGTGCCCGGGCCGAGGTGGGCCTCGTC
NM_019886.3       ATCTGCTCGCCGCCACTGTGTCCTGGCGCACCCCGTGCCCGGGCCGAGGTGGGCCTCGTC
                  ************************************************************

HsCD00351757      GAGGACACCGCCTGCGAGCGCAGCTGCCCACCCGTGGCGATACGCGCCCTGGAGGCCGAG
NM_019886.3       GAGGACACCGCCTGCGAGCGCAGCTGCCCACCCGTGGCGATACGCGCCCTGGAGGCCGAG
                  ************************************************************

HsCD00351757      TGCCGAAAGTACCCGGTGGTGGTCATCAAGGACGTGCGCCTGCTCGATCTGGGCGTGCTG
NM_019886.3       TGCCGAAAGTACCCGGTGGTGGTCATCAAGGACGTGCGCCTGCTCGATCTGGGCGTGCTG
                  ************************************************************

HsCD00351757      GTGCCCCTGTTGCGTGATCCAGGCCTCAACCTGAAGGTGGTGCAGCTTTTCCGCGACCCG
NM_019886.3       GTGCCCCTGTTGCGTGATCCAGGCCTCAACCTGAAGGTGGTGCAGCTTTTCCGCGACCCG
                  ************************************************************

HsCD00351757      AGGGCGGTGCACAACTCGCGCCTCAAGTCTAGGCAGGGACTGCTGCGCGAGAGCATCCAG
NM_019886.3       AGGGCGGTGCACAACTCGCGCCTCAAGTCTAGGCAGGGACTGCTGCGCGAGAGCATCCAG
                  ************************************************************

HsCD00351757      GTGCTGCGCACCCGCCAGAGGGGCGACCGCTTCCACCGTGTGCTGCTGGCGCACGGCGTG
NM_019886.3       GTGCTGCGCACCCGCCAGAGGGGCGACCGCTTCCACCGTGTGCTGCTGGCGCACGGCGTG
                  ************************************************************

HsCD00351757      GGTGCTCGCCCCGGGGGCCAGTCTCGCGCGCTGCCCGCCGCGCCGCGCGCCGATTTCTTC
NM_019886.3       GGTGCTCGCCCCGGGGGCCAGTCTCGCGCGCTGCCCGCCGCGCCGCGCGCCGATTTCTTC
                  ************************************************************

HsCD00351757      CTGACCGGTGCGCTCGAGGTGATCTGCGAAGCCTGGCTGCGCGATCTGCTTTTCGCGCGC
NM_019886.3       CTGACCGGTGCGCTCGAGGTGATCTGCGAAGCCTGGCTGCGCGATCTGCTTTTCGCGCGC
                  ************************************************************

HsCD00351757      GGCGCGCCCGCCTGGCTGCGGCGCCGCTACCTGAGGCTGCGCTATGAGGACCTGGTGCGG
NM_019886.3       GGCGCGCCCGCCTGGCTGCGGCGCCGCTACCTGAGGCTGCGCTATGAGGACCTGGTGCGG
                  ************************************************************

HsCD00351757      CAGCCACGCGCCCAGCTGCGCCGCCTGCTGCGCTTCTCCGGGCTACGCGCGCTCGCAGCG
NM_019886.3       CAGCCACGCGCCCAGCTGCGCCGCCTGCTGCGCTTCTCCGGGCTACGCGCGCTCGCAGCG
                  ************************************************************

HsCD00351757      CTCGATGCCTTCGCGCTCAACATGACTCGCGGCGCGGCCTACGGCGCCGACCGGCCCTTC
NM_019886.3       CTCGATGCCTTCGCGCTCAACATGACTCGCGGCGCGGCCTACGGCGCCGACCGGCCCTTC
                  ************************************************************

HsCD00351757      CACCTGTCAGCGCGCGACGCCCGGGAGGCGGTGCACGCCTGGCGCGAGCGCCTGAGCCGA
NM_019886.3       CACCTGTCAGCGCGCGACGCCCGGGAGGCGGTGCACGCCTGGCGCGAGCGCCTGAGCCGA
                  ************************************************************

HsCD00351757      GAGCAGGTGCGCCAGGTGGAGGCCGCCTGCGCTCCAGCCATGCGTCTGCTCGCCTACCCT
NM_019886.3       GAGCAGGTGCGCCAGGTGGAGGCCGCCTGCGCTCCAGCCATGCGTCTGCTCGCCTACCCT
                  ************************************************************

HsCD00351757      CGCAGCGGAGAGGAGGGCGACGCGGAGCAGCCCAGGGAAGGGGAGACGCCGCTGGAGATG
NM_019886.3       CGCAGCGGAGAGGAGGGCGACGCGGAGCAGCCCAGGGAAGGGGAGACGCCGCTGGAGATG
                  ************************************************************

HsCD00351757      GATGCCGACGGCGCCACGTGA
NM_019886.3       GATGCCGACGGCGCCACGTAG
                  *******************..

Pathways

Reactome :

Chondroitin sulfate biosynthesis

Reactome :

Chondroitin sulfate/dermatan sulfate metabolism

Reactome :

Disease

Reactome :

Glycogen storage diseases

Reactome :

Glycosaminoglycan metabolism

Reactome :

Metabolism

Reactome :

Metabolism of carbohydrates

Reactome :

MPS I - Hurler syndrome

Reactome :

MPS II - Hunter syndrome

Reactome :

MPS IIIA - Sanfilippo syndrome A

Reactome :

MPS IIIB - Sanfilippo syndrome B

Reactome :

MPS IIIC - Sanfilippo syndrome C

Reactome :

MPS IIID - Sanfilippo syndrome D

Reactome :

MPS IV - Morquio syndrome A

Reactome :

MPS IV - Morquio syndrome B

Reactome :

MPS IX - Natowicz syndrome

Reactome :

MPS VI - Maroteaux-Lamy syndrome

Reactome :

MPS VII - Sly syndrome

Reactome :

Mucopolysaccharidoses

Reactome :

Myoclonic epilepsy of Lafora

WikiPathway :

metapathway biotransformation

Protein

UniProt :

Q9NS84

HPRD :

02302

Clone

Cloning Information :

564364

Organism

Species Specific ID:

56548

Genome

Chromosome :

Map Location :

Xp11.23

Ensembl :

ENSG00000147119

Reagents

Labome :

CHST7-antibody