DNASU Plasmid | PPIL4 (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PPIL4

Gene Name: peptidylprolyl isomerase (cyclophilin)-like 4

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100068595)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351783

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1479nts

Open reading frame: 1 to 1479

Reference Sequence Alignment

ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGA

Protein sequence predicted based on nucleotide sequence

MAVLLETTLGDVVIDLYTEERPRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTGDPTGTG
RGGESIFGQLYGDQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLD
GVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPE
PTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDLPDADIKPPEN
VLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIEFEKEEDCEKAFFK
MDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDKVKPKQD
TKYDLILDEQAEDSKSSHSHTSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHY
EEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSP
KKSKDKEKSKYR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

PPIL4

Symbol Nomenclature :

PPIL4

SYNONYM :

HDCME13P

Designation :

Full Nomenclature :

peptidylprolyl isomerase (cyclophilin)-like 4

GENEID :

85313

Locus Tag :

RP11-703H16.1

GenBank Accession :

564390

HGNC :

15702

MIM :

607609

Vega :

OTTHUMG00000015788

Target GenBank:

GQ129365

Reference Sequence Alignment

  

HsCD00351783      ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
NM_139126.3       ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
                  ************************************************************

HsCD00351783      CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
NM_139126.3       CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
                  ************************************************************

HsCD00351783      CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
NM_139126.3       CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
                  ************************************************************

HsCD00351783      CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
NM_139126.3       CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
                  ************************************************************

HsCD00351783      GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
NM_139126.3       GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
                  ************************************************************

HsCD00351783      AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
NM_139126.3       AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
                  ************************************************************

HsCD00351783      GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
NM_139126.3       GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
                  ************************************************************

HsCD00351783      GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
NM_139126.3       GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
                  ************************************************************

HsCD00351783      ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
NM_139126.3       ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
                  ************************************************************

HsCD00351783      CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
NM_139126.3       CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
                  ************************************************************

HsCD00351783      AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
NM_139126.3       AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
                  ************************************************************

HsCD00351783      GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
NM_139126.3       GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
                  ************************************************************

HsCD00351783      GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
NM_139126.3       GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
                  ************************************************************

HsCD00351783      TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
NM_139126.3       TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
                  ************************************************************

HsCD00351783      CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
NM_139126.3       CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
                  ************************************************************

HsCD00351783      ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
NM_139126.3       ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
                  ************************************************************

HsCD00351783      AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
NM_139126.3       AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
                  ************************************************************

HsCD00351783      AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
NM_139126.3       AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
                  ************************************************************

HsCD00351783      ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
NM_139126.3       ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
                  ************************************************************

HsCD00351783      ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
NM_139126.3       ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
                  ************************************************************

HsCD00351783      TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
NM_139126.3       TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
                  ************************************************************

HsCD00351783      GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
NM_139126.3       GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
                  ************************************************************

HsCD00351783      CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
NM_139126.3       CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
                  ************************************************************

HsCD00351783      TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
NM_139126.3       TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
                  ************************************************************

HsCD00351783      AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGA
NM_139126.3       AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGA
                  ***************************************