DNASU Plasmid | ERO1L (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ERO1L

Gene Name: ERO1-like (S. cerevisiae)

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100068696)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351890

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1404nts

Open reading frame: 1 to 1404

Reference Sequence Alignment

ATGGGCCGCGGCTGGGGATTCTTGTTTGGCCTCCTGGGCGCCGTGTGGCTGCTCAGCTCG
GGCCACGGAGAGGAGCAGCCCCCGGAGACAGCGGCACAGAGGTGCTTCTGCCAGGTTAGT
GGTTACTTGGATGATTGTACCTGTGATGTTGAAACCATTGATAGATTTAATAACTACAGG
CTTTTCCCAAGACTACAAAAACTTCTTGAAAGTGACTACTTTAGGTATTACAAGGTAAAC
CTGAAGAGGCCGTGTCCTTTCTGGAATGACATCAGCCAGTGTGGAAGAAGGGACTGTGCT
GTCAAACCATGTCAATCTGATGAAGTTCCTGATGGAATTAAATCTGCGAGCTACAAGTAT
TCTGAAGAAGCCAATAATCTCATTGAAGAATGTGAACAAGCTGAACGACTTGGAGCAGTG
GATGAATCTCTGAGTGAGGAAACACAGAAGGCTGTTCTTCAGTGGACCAAGCATGATGAT
TCTTCAGATAACTTCTGTGAAGCTGATGACATTCAGTCCCCTGAAGCTGAATATGTAGAT
TTGCTTCTTAATCCTGAGCGCTACACTGGTTACAAGGGACCAGATGCTTGGAAAATATGG
AATGTCATCTACGAAGAAAACTGTTTTAAGCCACAGACAATTAAAAGACCTTTAAATCCT
TTGGCTTCTGGTCAAGGGACAAGTGAAGAGAACACTTTTTACAGTTGGCTAGAAGGTCTC
TGTGTAGAAAAAAGAGCATTCTACAGACTTATATCTGGCCTACATGCAAGCATTAATGTG
CATTTGAGTGCAAGATATCTTTTACAAGAGACCTGGTTAGAAAAGAAATGGGGACACAAC
ATTACAGAATTTCAACAGCGATTTGATGGAATTTTGACTGAAGGAGAAGGTCCAAGAAGG
CTTAAGAACTTGTATTTTCTCTACTTAATAGAACTAAGGGCTTTATCCAAAGTGTTACCA
TTCTTCGAGCGCCCAGATTTTCAACTCTTTACTGGAAATAAAATTCAGGATGAGGAAAAC
AAAATGTTACTTCTGGAAATACTTCATGAAATCAAGTCATTTCCTTTGCATTTTGATGAG
AATTCATTTTTTGCTGGGGATAAAAAAGAAGCACACAAACTAAAGGAGGACTTTCGACTG
CATTTTAGAAATATTTCAAGAATTATGGATTGTGTTGGTTGTTTTAAATGTCGTCTGTGG
GGAAAGCTTCAGACTCAGGGTTTGGGCACTGCTCTGAAGATCTTATTTTCTGAGAAATTG
ATAGCAAATATGCCAGAAAGTGGACCTAGTTATGAATTCCATCTAACCAGACAAGAAATA
GTATCATTATTCAACGCATTTGGAAGAATTTCTACAAGTGTGAAAGAATTAGAAAACTTC
AGGAACTTGTTACAGAATATTCAT

Protein sequence predicted based on nucleotide sequence

MGRGWGFLFGLLGAVWLLSSGHGEEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYR
LFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKY
SEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVD
LLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGL
CVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRR
LKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDE
NSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKL
IANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

ERO1L

Symbol Nomenclature :

ERO1L

SYNONYM :

ERO1-alpha; ERO1A; ERO1LA

Designation :

ERO1-L|ERO1-L-alpha|ERO1-like protein alpha|endoplasmic oxidoreductin-1-like protein|oxidoreductin-1-L-alpha

Full Nomenclature :

ERO1-like (S. cerevisiae)

GENEID :

30001

Locus Tag :

UNQ434/PRO865

GenBank Accession :

564497

HGNC :

13280

MIM :

615435

Target GenBank:

GQ129372

Reference Sequence Alignment

  

HsCD00351890      ATGGGCCGCGGCTGGGGATTCTTGTTTGGCCTCCTGGGCGCCGTGTGGCTGCTCAGCTCG
NM_014584.1       ATGGGCCGCGGCTGGGGATTCTTGTTTGGCCTCCTGGGCGCCGTGTGGCTGCTCAGCTCG
                  ************************************************************

HsCD00351890      GGCCACGGAGAGGAGCAGCCCCCGGAGACAGCGGCACAGAGGTGCTTCTGCCAGGTTAGT
NM_014584.1       GGCCACGGAGAGGAGCAGCCCCCGGAGACAGCGGCACAGAGGTGCTTCTGCCAGGTTAGT
                  ************************************************************

HsCD00351890      GGTTACTTGGATGATTGTACCTGTGATGTTGAAACCATTGATAGATTTAATAACTACAGG
NM_014584.1       GGTTACTTGGATGATTGTACCTGTGATGTTGAAACCATTGATAGATTTAATAACTACAGG
                  ************************************************************

HsCD00351890      CTTTTCCCAAGACTACAAAAACTTCTTGAAAGTGACTACTTTAGGTATTACAAGGTAAAC
NM_014584.1       CTTTTCCCAAGACTACAAAAACTTCTTGAAAGTGACTACTTTAGGTATTACAAGGTAAAC
                  ************************************************************

HsCD00351890      CTGAAGAGGCCGTGTCCTTTCTGGAATGACATCAGCCAGTGTGGAAGAAGGGACTGTGCT
NM_014584.1       CTGAAGAGGCCGTGTCCTTTCTGGAATGACATCAGCCAGTGTGGAAGAAGGGACTGTGCT
                  ************************************************************

HsCD00351890      GTCAAACCATGTCAATCTGATGAAGTTCCTGATGGAATTAAATCTGCGAGCTACAAGTAT
NM_014584.1       GTCAAACCATGTCAATCTGATGAAGTTCCTGATGGAATTAAATCTGCGAGCTACAAGTAT
                  ************************************************************

HsCD00351890      TCTGAAGAAGCCAATAATCTCATTGAAGAATGTGAACAAGCTGAACGACTTGGAGCAGTG
NM_014584.1       TCTGAAGAAGCCAATAATCTCATTGAAGAATGTGAACAAGCTGAACGACTTGGAGCAGTG
                  ************************************************************

HsCD00351890      GATGAATCTCTGAGTGAGGAAACACAGAAGGCTGTTCTTCAGTGGACCAAGCATGATGAT
NM_014584.1       GATGAATCTCTGAGTGAGGAAACACAGAAGGCTGTTCTTCAGTGGACCAAGCATGATGAT
                  ************************************************************

HsCD00351890      TCTTCAGATAACTTCTGTGAAGCTGATGACATTCAGTCCCCTGAAGCTGAATATGTAGAT
NM_014584.1       TCTTCAGATAACTTCTGTGAAGCTGATGACATTCAGTCCCCTGAAGCTGAATATGTAGAT
                  ************************************************************

HsCD00351890      TTGCTTCTTAATCCTGAGCGCTACACTGGTTACAAGGGACCAGATGCTTGGAAAATATGG
NM_014584.1       TTGCTTCTTAATCCTGAGCGCTACACTGGTTACAAGGGACCAGATGCTTGGAAAATATGG
                  ************************************************************

HsCD00351890      AATGTCATCTACGAAGAAAACTGTTTTAAGCCACAGACAATTAAAAGACCTTTAAATCCT
NM_014584.1       AATGTCATCTACGAAGAAAACTGTTTTAAGCCACAGACAATTAAAAGACCTTTAAATCCT
                  ************************************************************

HsCD00351890      TTGGCTTCTGGTCAAGGGACAAGTGAAGAGAACACTTTTTACAGTTGGCTAGAAGGTCTC
NM_014584.1       TTGGCTTCTGGTCAAGGGACAAGTGAAGAGAACACTTTTTACAGTTGGCTAGAAGGTCTC
                  ************************************************************

HsCD00351890      TGTGTAGAAAAAAGAGCATTCTACAGACTTATATCTGGCCTACATGCAAGCATTAATGTG
NM_014584.1       TGTGTAGAAAAAAGAGCATTCTACAGACTTATATCTGGCCTACATGCAAGCATTAATGTG
                  ************************************************************

HsCD00351890      CATTTGAGTGCAAGATATCTTTTACAAGAGACCTGGTTAGAAAAGAAATGGGGACACAAC
NM_014584.1       CATTTGAGTGCAAGATATCTTTTACAAGAGACCTGGTTAGAAAAGAAATGGGGACACAAC
                  ************************************************************

HsCD00351890      ATTACAGAATTTCAACAGCGATTTGATGGAATTTTGACTGAAGGAGAAGGTCCAAGAAGG
NM_014584.1       ATTACAGAATTTCAACAGCGATTTGATGGAATTTTGACTGAAGGAGAAGGTCCAAGAAGG
                  ************************************************************

HsCD00351890      CTTAAGAACTTGTATTTTCTCTACTTAATAGAACTAAGGGCTTTATCCAAAGTGTTACCA
NM_014584.1       CTTAAGAACTTGTATTTTCTCTACTTAATAGAACTAAGGGCTTTATCCAAAGTGTTACCA
                  ************************************************************

HsCD00351890      TTCTTCGAGCGCCCAGATTTTCAACTCTTTACTGGAAATAAAATTCAGGATGAGGAAAAC
NM_014584.1       TTCTTCGAGCGCCCAGATTTTCAACTCTTTACTGGAAATAAAATTCAGGATGAGGAAAAC
                  ************************************************************

HsCD00351890      AAAATGTTACTTCTGGAAATACTTCATGAAATCAAGTCATTTCCTTTGCATTTTGATGAG
NM_014584.1       AAAATGTTACTTCTGGAAATACTTCATGAAATCAAGTCATTTCCTTTGCATTTTGATGAG
                  ************************************************************

HsCD00351890      AATTCATTTTTTGCTGGGGATAAAAAAGAAGCACACAAACTAAAGGAGGACTTTCGACTG
NM_014584.1       AATTCATTTTTTGCTGGGGATAAAAAAGAAGCACACAAACTAAAGGAGGACTTTCGACTG
                  ************************************************************

HsCD00351890      CATTTTAGAAATATTTCAAGAATTATGGATTGTGTTGGTTGTTTTAAATGTCGTCTGTGG
NM_014584.1       CATTTTAGAAATATTTCAAGAATTATGGATTGTGTTGGTTGTTTTAAATGTCGTCTGTGG
                  ************************************************************

HsCD00351890      GGAAAGCTTCAGACTCAGGGTTTGGGCACTGCTCTGAAGATCTTATTTTCTGAGAAATTG
NM_014584.1       GGAAAGCTTCAGACTCAGGGTTTGGGCACTGCTCTGAAGATCTTATTTTCTGAGAAATTG
                  ************************************************************

HsCD00351890      ATAGCAAATATGCCAGAAAGTGGACCTAGTTATGAATTCCATCTAACCAGACAAGAAATA
NM_014584.1       ATAGCAAATATGCCAGAAAGTGGACCTAGTTATGAATTCCATCTAACCAGACAAGAAATA
                  ************************************************************

HsCD00351890      GTATCATTATTCAACGCATTTGGAAGAATTTCTACAAGTGTGAAAGAATTAGAAAACTTC
NM_014584.1       GTATCATTATTCAACGCATTTGGAAGAATTTCTACAAGTGTGAAAGAATTAGAAAACTTC
                  ************************************************************

HsCD00351890      AGGAACTTGTTACAGAATATTCAT---
NM_014584.1       AGGAACTTGTTACAGAATATTCATTAA
                  ************************

Pathways

Reactome :

Cellular responses to stress

Reactome :

Detoxification of Reactive Oxygen Species

Reactome :

Insulin processing

Reactome :

Metabolism of proteins

Reactome :

Peptide hormone metabolism

Protein

UniProt :

Q96HE7

HPRD :

13278

Clone

Cloning Information :

564497

Organism

Species Specific ID:

30001

Genome

Chromosome :

Map Location :

14q22.1

Reagents

Labome :

ERO1L-antibody