Gene:
Gene Symbol:
FRMD4B
Gene Name:
FERM domain containing 4B
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069009)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00353935
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTCGGTGTTCATGTGTGGCGTGGAGGACCTGCTGTTCAGCGGCAGCCGCTTCGTA
TGGAACTTGACCGTGTCCACGCTGCGGAGATGGTACACGGAGAGGCTGCGGGCTTGCCAC
CAGGTGCTGCGGACGTGGTGCGGGCTGCAGGACGTGTACCAGATGACAGAAGGCAGGCAC
TGCCAGGTGCACCTCCTGGATGATAGGAGACTGGAGCTGCTGGTTCAGCCCAAACTTCTA
GCAAGAGAGTTGCTGGACCTAGTGGCTTCACATTTCAACCTGAAAGAAAAGGAGTATTTT
GGAATAACATTCATAGATGACACAGGTCAGCAGAACTGGCTGCAGTTAGATCACCGAGTT
CTTGACCACGATTTGCCCAAGAAACCAGGCCCAACCATTTTGCACTTTGCTGTGAGGTTT
TACATTGAGAGCATATCGTTTTTAAAGGATAAAACCACCGTGGAGCTGTTTTTCCTGAAT
GCAAAGGCCTGTGTGCACAAGGGGCAAATCGAAGTAGAGAGCGAAACCATCTTCAAGTTA
GCAGCGTTTATTTTACAGGAAGCCAAGGGAGATTATACCAGTGATGAAAATGCCAGGAAA
GATTTAAAGACATTACCAGCCTTTCCAACCAAAACTCTTCAGGAGCATCCATCCCTTGCC
TACTGTGAGGACAGGGTAATTGAGCATTATTTGAAAATCAAAGGTCTCACTCGAGGTCAA
GCTGTGGTTCAGTATATGAAAATAGTAGAAGCTCTACCGACTTACGGTGTCCATTATTAT
GCAGTAAAGGATAAGCAAGGACTTCCTTGGTGGCTTGGAATAAGCTATAAGGGAATTGGC
CAATATGATATACAAGACAAGGTGAAGCCTCGGAAGTTATTCCAATGGAAACAGCTGGAG
AACTTATATTTCCGTGAGAAAAAATTTGCTGTTGAAGTTCATGATCCACGAAGGATTTCA
GTTTCAAGAAGAACCTTTGGGCAAAGTGGCTTGTTTGTGCAAACATGGTATGCTAACTCT
TCTCTCATCAAGTCCATTTGGGTAATGGCAATTAGTCAGCATCAGTTTTACTTGGACCGG
AAGCAAAGCAAAGCAAAAATTCCTTCAGCCAGGAGTTTAGATGAGATTGCAATGGATTTG
ACAGAGACAGGAACACAAAGGGCCTCCAAGCTGGTGACACTGGAGGCGAAAAGTCAGTTC
ATCATGGCAAGTAATGGCAGTTTAATCTCCTCAGGTTCTCAAGACTCAGAAGTTAGTGAA
GAGCAAAAGAGAGAAAAAATCCTTGAACTAAAGAAGAAGGAGAAACTATTACAAGAAAAA
CTTCTGAAAAAAGTTGAGGAGCTTAAGAAGATCTGTCTGCGGGAGGCTGAGCTCACAGGC
AAAATGCCAAAGGAGTATCCCCTGAACATAGGCGAGAAGCCTCCTCAGGTCAGAAGACGT
GTGGGTACTGCGTTCAAATTAGATGACAACTTGCTGCCGAGTGAAGAAGATCCTGCTTTG
CAAGAACTGGAGAGTAATTTTCTAATACAACAAAAGCTGGTGGAAGCTGCAAAGAAACTT
GCCAATGAGCCAGACCTTTGTAAAACTGTGAAGAAAAAGCGAAAGCAAGATTACACAGAT
GCGATGAAAAAGCTTCAGGAGATTGAAAATGCAATAAACGAATACCGAATTAGGTGTGGA
AAGAAACCCAGCCAGAAAGCAACAGTGTTACCAGAAGACATAATCCCCTCAGAGAGTAGC
TCTTTGTCTGACACCACCACCTATGATGATCCCAGTGATGCCTTCACTTTTCCTGGGCAG
CGATCAAGTTCAGTACCTCATTCTCCAAGAATTCTTCCCCCCAAGTCTCTTGGTATTGAG
CGAATCCATTTCAGAAAGTCGTCCATCAATGAACAGTTTGTGGATACCAGGCAGTCCAGA
GAAATGCTGTCCACACACAGCAGCCCTTACAAAACTCTGGAGAGGCGGCCCCAGGGAGGA
CGAAGCATGCCCACCACGCCAGTTCTTACCCGAAACGCCTACAGCAGCAGCCACTTGGAA
CCCGAATCTTCATCTCAGCACTGCCGCCAGCGGAGTGGAAGCCTGGAGTCCCAGTCCCAC
CTGCTCTCCGAGATGGACAGCGATAAGCCATTTTTCTCCCTCTCCAAATCCCAAAGAAGC
AGCAGCACAGAAATCCTCGATGACGGGTCTTCTTATACAAGCCAATCAAGCACAGAGTAT
TACTGTGTGACACCAGTTACCGGCCCCTATTACACCACCCAGACCCTGGACACTCGCACC
AGGGGTCGGAGGAGGTCAAAGAAACAGAATGTTTCTACTTCAAATTCAGGAAGCATGCCC
AACCTAGCACAAAAGGATAGTTTGAGGAATGGTGTTTACTCAAAGAGTCAGGAGCCACCG
TCTTCCAGTTACTACATTGCCGGGTACACACCCTATGCAGAGTGTGACTTTTATTACAGT
GGTGGTTATGTCTATGAGAATGACACCGAGGGACAGTATAGTGTCAACCCTTCCTACCGG
TCCTCAGCCCACTATGGATATGAGCGCCAGAGGGACTACAGCAGATCCTTTCACGAAGAT
GAGGTCGACCGGGTACCCCATAACCCATATGCAACTCTCCGGCTGCCAAGGAAGGCTGCT
GCAAAATCGGAGCACATCACCAAAAACATCCACAAGGCCTTAGTTGCCGAGCACTTGCGT
GGCTGGTACCAGCGGGCCTCTGGGCAGAAGGATCAGGGACACAGCCCGCAGACCAGCTTT
GACTCAGACAGGGGATCACAGAGATGCCTGGGGTTTGCGGGGCTGCAAGTACCTTGTTCT
CCAAGCAGTCGTGCATCCTCGTACTCTTCAGTGTCTTCTACAAATGCTTCTGGGAACTGG
AGGACCCAGTTAACCATAGGGCTGTCGGATTACGAGACTCCAGCACATTCTTCTTACACC
AGCTGCTATGGCAATGTCTATAATCCTTTACCCTCTCCAAGCAGACAATACACAGAAATC
AGTCAACTGGATGGTACAGATGGAAACCAGCTGGAAGACAACCTGGAGAGTAGTGAGCAG
AGACTCTTTTGGCATGAAGATTCAAAGCCTGGAACATTAGTC
Protein sequence predicted based on nucleotide sequence
MASVFMCGVEDLLFSGSRFVWNLTVSTLRRWYTERLRACHQVLRTWCGLQDVYQMTEGRH CQVHLLDDRRLELLVQPKLLARELLDLVASHFNLKEKEYFGITFIDDTGQQNWLQLDHRV LDHDLPKKPGPTILHFAVRFYIESISFLKDKTTVELFFLNAKACVHKGQIEVESETIFKL AAFILQEAKGDYTSDENARKDLKTLPAFPTKTLQEHPSLAYCEDRVIEHYLKIKGLTRGQ AVVQYMKIVEALPTYGVHYYAVKDKQGLPWWLGISYKGIGQYDIQDKVKPRKLFQWKQLE NLYFREKKFAVEVHDPRRISVSRRTFGQSGLFVQTWYANSSLIKSIWVMAISQHQFYLDR KQSKAKIPSARSLDEIAMDLTETGTQRASKLVTLEAKSQFIMASNGSLISSGSQDSEVSE EQKREKILELKKKEKLLQEKLLKKVEELKKICLREAELTGKMPKEYPLNIGEKPPQVRRR VGTAFKLDDNLLPSEEDPALQELESNFLIQQKLVEAAKKLANEPDLCKTVKKKRKQDYTD AMKKLQEIENAINEYRIRCGKKPSQKATVLPEDIIPSESSSLSDTTTYDDPSDAFTFPGQ RSSSVPHSPRILPPKSLGIERIHFRKSSINEQFVDTRQSREMLSTHSSPYKTLERRPQGG RSMPTTPVLTRNAYSSSHLEPESSSQHCRQRSGSLESQSHLLSEMDSDKPFFSLSKSQRS SSTEILDDGSSYTSQSSTEYYCVTPVTGPYYTTQTLDTRTRGRRRSKKQNVSTSNSGSMP NLAQKDSLRNGVYSKSQEPPSSSYYIAGYTPYAECDFYYSGGYVYENDTEGQYSVNPSYR SSAHYGYERQRDYSRSFHEDEVDRVPHNPYATLRLPRKAAAKSEHITKNIHKALVAEHLR GWYQRASGQKDQGHSPQTSFDSDRGSQRCLGFAGLQVPCSPSSRASSYSSVSSTNASGNW RTQLTIGLSDYETPAHSSYTSCYGNVYNPLPSPSRQYTEISQLDGTDGNQLEDNLESSEQ RLFWHEDSKPGTLV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
