DNASU Plasmid | UBE2Q1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: UBE2Q1

Gene Name: ubiquitin-conjugating enzyme E2Q family member 1

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100069011)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00353937

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1269nts

Open reading frame: 1 to 1269

Reference Sequence Alignment

ATGCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAGCTGGGGGGC
CAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGGCCCGGGGCCGGGGCCCTGC
CTGAGGCGAGAGCTGAAGCTGCTCGAGTCCATCTTCCACCGCGGCCACGAGCGCTTCCGC
ATTGCCAGCGCCTGCCTGGACGAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGG
GCCGGGGCGGGGGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGAT
CCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCCATCTGGTCG
GTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAAA
GGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGTGTAAACTCTAT
AACCTCCCTCAGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGC
ACACAGGAAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGAC
TTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCTGAAGATGAT
GGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAAAAAGAACCAGAGGCAAGAT
TACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGATGAAGGAGCTC
AGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAAT
GACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCAC
AACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTTAACTTTTCC
TTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGTTGTGTCTCCAGTCCTCTCT
GGAGGGTATGTTCTGGGCGGAGGGGCCATCTGCATGGAACTTCTCACCAAACAGGGCTGG
AGCAGTGCCTACTCCATAGAGTCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGG
AAAGCACGAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAG
TCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCAAAAGAA
GACGGCTAG

Protein sequence predicted based on nucleotide sequence

MQQPQPQGQQQPGPGQQLGGQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFR
IASACLDELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPPIWS
VESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPDVEMLDQPLPAEQC
TQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSEDDGIGKENLAILEKIKKNQRQD
YLNGAVSGSVQATDRLMKELRDIYRSQSFKGGNYAVELVNDSLYDWNVKLLKVDQDSALH
NDLQILKEKEGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGW
SSAYSIESVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKE
DG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

UBE2Q1

Symbol Nomenclature :

UBE2Q1

SYNONYM :

GTAP; NICE-5; UBE2Q

Designation :

Full Nomenclature :

ubiquitin-conjugating enzyme E2Q family member 1

GENEID :

55585

Locus Tag :

PRO3094

GenBank Accession :

571939

HGNC :

15698

Vega :

OTTHUMG00000037265

Target GenBank:

BC172317

Reference Sequence Alignment

  

HsCD00353937      ATGCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAGCTGGGGGGC
NM_017582.6       ATGCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAGCTGGGGGGC
                  ************************************************************

HsCD00353937      CAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGGCCCGGGGCCGGGGCCCTGC
NM_017582.6       CAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGGCCCGGGGCCGGGGCCCTGC
                  ************************************************************

HsCD00353937      CTGAGGCGAGAGCTGAAGCTGCTCGAGTCCATCTTCCACCGCGGCCACGAGCGCTTCCGC
NM_017582.6       CTGAGGCGAGAGCTGAAGCTGCTCGAGTCCATCTTCCACCGCGGCCACGAGCGCTTCCGC
                  ************************************************************

HsCD00353937      ATTGCCAGCGCCTGCCTGGACGAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGG
NM_017582.6       ATTGCCAGCGCCTGCCTGGACGAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGG
                  ************************************************************

HsCD00353937      GCCGGGGCGGGGGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGAT
NM_017582.6       GCCGGGGCGGGGGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGAT
                  ************************************************************

HsCD00353937      CCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCCATCTGGTCG
NM_017582.6       CCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCCATCTGGTCG
                  ************************************************************

HsCD00353937      GTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAAA
NM_017582.6       GTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAAA
                  ************************************************************

HsCD00353937      GGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGTGTAAACTCTAT
NM_017582.6       GGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGTGTAAACTCTAT
                  ************************************************************

HsCD00353937      AACCTCCCTCAGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGC
NM_017582.6       AACCTCCCTCAGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGC
                  ************************************************************

HsCD00353937      ACACAGGAAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGAC
NM_017582.6       ACACAGGAAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGAC
                  ************************************************************

HsCD00353937      TTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCTGAAGATGAT
NM_017582.6       TTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCTGAAGATGAT
                  ************************************************************

HsCD00353937      GGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAAAAAGAACCAGAGGCAAGAT
NM_017582.6       GGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAAAAAGAACCAGAGGCAAGAT
                  ************************************************************

HsCD00353937      TACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGATGAAGGAGCTC
NM_017582.6       TACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGATGAAGGAGCTC
                  ************************************************************

HsCD00353937      AGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAAT
NM_017582.6       AGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAAT
                  ************************************************************

HsCD00353937      GACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCAC
NM_017582.6       GACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCAC
                  ************************************************************

HsCD00353937      AACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTTAACTTTTCC
NM_017582.6       AACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTTAACTTTTCC
                  ************************************************************

HsCD00353937      TTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGTTGTGTCTCCAGTCCTCTCT
NM_017582.6       TTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGTTGTGTCTCCAGTCCTCTCT
                  ************************************************************

HsCD00353937      GGAGGGTATGTTCTGGGCGGAGGGGCCATCTGCATGGAACTTCTCACCAAACAGGGCTGG
NM_017582.6       GGAGGGTATGTTCTGGGCGGAGGGGCCATCTGCATGGAACTTCTCACCAAACAGGGCTGG
                  ************************************************************

HsCD00353937      AGCAGTGCCTACTCCATAGAGTCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGG
NM_017582.6       AGCAGTGCCTACTCCATAGAGTCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGG
                  ************************************************************

HsCD00353937      AAAGCACGAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAG
NM_017582.6       AAAGCACGAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAG
                  ************************************************************

HsCD00353937      TCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCAAAAGAA
NM_017582.6       TCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCAAAAGAA
                  ************************************************************

HsCD00353937      GACGGCTAG
NM_017582.6       GACGGCTAA
                  ********.