Gene:
Gene Symbol:
ANKRD12
Gene Name:
ankyrin repeat domain 12
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069232)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00353966
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCCAAATCTGGGTTCACAAAACCAATTCAGAGTGAAAATTCTGACAGTGACAGCAAT
ATGGTAGAGAAACCATATGGAAGAAAGAGTAAAGACAAGATTGCATCCTACAGCAAAACT
CCAAAAATTGAACGAAGTGATGTGAGCAAGGAGATGAAAGAGAAATCATCCATGAAACGT
AAACTTCCTTTTACTATTAGCCCATCAAGAAATGAAGAACGAGATTCAGACACAGATTCA
GATCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTATATCTTCTTACAGGACATAC
TCAGAGAAAGAAGGTCCAGAAAAGAAGAAGACAAAAAAGGAAGCTGGAAATAAGAAATCC
ACACCAGTTAGCATTCTTTTTGGTTATCCACTCTCTGAGCGAAAACAGATGGCACTTCTT
ATGCAGATGACAGCAAGAGACAACAGTCCAGATTCCACACCAAATCATCCATCACAAACA
ACGCCTGCCCAAAAGAAAACTCCCAGTTCTTCATCTCGACAGAAAGATAAAGTTAATAAA
AGAAATGAACGTGGTGAAACTCCTTTACACATGGCTGCTATTCGAGGAGATGTGAAACAA
GTTAAAGAATTAATAAGTTTAGGGGCAAATGTGAATGTGAAAGATTTTGCAGGTTGGACA
CCACTGCATGAAGCTTGCAATGTTGGATATTACGATGTTGCTAAGATACTTATAGCAGCT
GGAGCAGATGTTAACACACAAGGATTAGATGATGACACTCCACTCCATGATTCTGCTAGT
AGTGGGCACAGAGATATAGTAAAGCTGTTACTTCGTCACGGTGGAAATCCATTTCAAGCT
AATAAACATGGGGAGCGTCCAGTGGATGTAGCAGAAACAGAGGAGTTGGAGTTGCTACTA
AAAAGAGAGGTGCCTTTATCTGATGATGATGAAAGTTACACAGATTCCGAAGAGGCTCAA
TCTGTAAATCCTTCTAGTGTTGATGAAAATATTGACTCTGAAACAGAGAAAGACTCTCTC
ATCTGTGAAAGTAAACAGATACTTCCCAGTAAAACACCTCTTCCATCTGCCCTTGATGAG
TATGAGTTCAAAGATGATGATGATGAAGAAATTAATAAGATGATTGATGATAGGCATATT
CTTAGGAAAGAACAACGAAAAGAAAATGAACCTGAAGCAGAAAAAACTCATTTATTTGCA
AAACAGGAGAAAGCCTTCTATCCTAAATCATTTAAAAGTAAAAAACAAAAGCCATCTAGG
GTCTTATATTCAAGTACTGAAAGTTCTGATGAAGAAGCTCTTCAGAATAAAAAGATTTCT
ACTTCATGTTCCGTCATCCCTGAAACATCAAATTCTGATATGCAAACCAAAAAGGAATAT
GTAGTTTCAGGTGAACACAAACAGAAAGGCAAAGTTAAAAGAAAATTGAAAAATCAGAAT
AAAAATAAAGAGAACCAAGAGCTAAAGCAAGAAAAGGAAGGAAAAGAAAATACAAGAATA
ACAAACTTGACAGTAAATACTGGACTAGATTGTTCAGAAAAGACCAGAGAGGAGGGGAAC
TTTAGGAAATCTTTTAGCCCAAAAGATGATACTTCATTACATTTATTTCATATTTCCACT
GGTAAATCTCCCAAACATTCTTGTGGATTAAGTGAAAAACAGTCAACACCACTAAAACAA
GAACATACTAAAACATGTTTATCACCAGGAAGTTCTGAAATGTCATTACAGCCTGATCTT
GTTCGGTATGATAATACAGAATCTGAATTCTTGCCAGAAAGTTCAAGTGTAAAATCTTGT
AAGCATAAGGAAAAAAGCAAACATCAGAAAGATTTCCACTTAGAATTTGGTGAAAAATCA
AATGCCAAAATAAAGGATGAAGATCATAGTCCAACATTTGAAAATTCAGATTGCACACTG
AAAAAAATGGATAAAGAAGGTAAAACATTAAAAAAACATAAATTGAAGCATAAAGAGAGG
GAAAAAGAAAAGCATAAAAAAGAAATTGAAGGTGAAAAGGAAAAATACAAAACTAAGGAT
AGTGCCAAAGAACTGCAGAGGAGTGTGGAATTTGATAGAGAATTTTGGAAAGAGAATTTT
TTTAAAAGTGATGAAACTGAAGATCTCTTTTTAAATATGGAACATGAATCCTTAACATTA
GAAAAAAAATCAAAATTGGAAAAAAACATCAAAGATGATAAATCAACCAAGGAAAAGCAT
GTGTCAAAAGAGAGGAACTTTAAAGAGGAACGAGACAAGATTAAAAAGGAAAGCGAGAAA
TCTTTTAGGGAGGAAAAAATAAAAGATCTAAAAGAAGAGAGAGAAAACATACCCACAGAT
AAAGACTCAGAATTTACTTCTTTGGGTATGAGTGCCATTGAGGAATCTATAGGGCTTCAT
TTAGTGGAAAAGGAAATAGACATTGAAAAACAAGAAAAGCATATAAAGGAAAGTAAAGAA
AAACCTGAGAAGCGATCTCAAATTAAAGAAAAGGACATTGAGAAGATGGAAAGAAAAACC
TTTGAAAAAGAAAAGAAGATAAAACATGAGCATAAGTCAGAAAAAGACAAATTAGATCTT
AGTGAATGTGTTGATAAAATAAAAGAAAAGGACAAGCTATATTCGCATCACACAGAAAAA
TGCCATAAAGAAGGTGAGAAGAGCAAAAATACTGCTGCTATTAAAAAAACTGACGACAGA
GAGAAAAGTAGAGAAAAGATGGATAGGAAACATGACAAAGAAAAGCCTGAAAAAGAGAGG
CATCTAGCAGAAAGCAAAGAAAAGCACTTGATGGAGAAAAAAAATAAACAATCAGATAAT
AGTGAATACAGTAAATCAGAAAAAGGCAAAAATAAAGAAAAAGACAGGGAGCTAGATAAA
AAGGAAAAATCTAGAGATAAAGAAAGTATAAATATAACTAACTCCAAACACATACAGGAA
GAAAAAAAATCAAGTATAGTAGACGGTAATAAAGCACAACATGAAAAACCCTTATCCCTT
AAAGAAAAAACAAAAGATGAACCTTTGAAAACTCCAGATGGAAAAGAAAAAGATAAAAAA
GATAAAGATATAGATAGATACAAAGAACGAGACAAACATAAAGATAAAATTCAAATAAAT
AGCTTACTCAAACTAAAATCTGAAGCAGATAAGCCTAAACCTAAGTCATCACCAGCATCA
AAAGATACCCGACCTAAAGAAAAGAGGTTAGTGAATGATGATTTAATGCAGACAAGTTTT
GAACGAATGCTAAGCCTTAAAGACCTAGAAATAGAACAGTGGCACAAAAAACATAAGGAA
AAAATTAAGCAAAAAGAAAAGGAACGGTTGAGAAACCGAAACTGTTTAGAACTTAAAATA
AAAGATAAAGAAAAAACAAAGCATACACCAACTGAATCCAAAAATAAAGAACTTACTAGG
TCAAAGAGTTCAGAAGTGACTGATGCATATACCAAGGAGAAACAACCTAAAGATGCTGTA
AGTAACAGATCACAATCTGTTGACACCAAAAATGTAATGACTTTAGGGAAGTCATCTTTT
GTTTCAGATAATAGCTTAAACAGGTCTCCTAGATCAGAAAATGAAAAGCCGGGTCTCAGC
TCCAGATCTGTATCCATGATTTCTGTTGCTAGTTCAGAAGATTCCTGCCATACTACAGTG
ACAACCCCAAGGCCTCCAGTTGAGTATGACTCTGACTTTATGTTAGAGAGTTCAGAATCC
CAAATGTCCTTTTCCCAGTCACCTTTTTTGTCAATTGCCAAATCTCCTGCTCTTCATGAA
AGGGAATTGGACAGCCTGGCTGACTTGCCGGAGCGGATTAAACCACCATATGCAAACAGA
CTTTCAACATCCCATCTTAGGTCATCTTCTGTAGAAGATGTTAAACTAATTATAAGCGAG
GGGAGACCTACCATAGAAGTTCGAAGATGTAGCATGCCTTCTGTCATTTGTGAACATACC
AAACAATTCCAAACAATATCAGAAGAGAGCAATCAAGGTAGCTTATTAACTGTGCCAGGA
GATACTAGTCCTTCTCCCAAACCTGAGGTATTCTCAAATGTGCCTGAAAGAGACCTTTCA
AATGTATCTAACATACATTCCAGTTTTGCAACTTCTCCAACTGGAGCTTCAAACAGCAAG
TATGTTTCAGCTGATAGAAATCTCATCAAGAATACTGCCCCAGTGAACACTGTAATGGAC
AGTCCAGTGCATTTAGAGCCATCTAGTCAGGTTGGTGTGATCCAGAATAAATCATGGGAG
ATGCCTGTTGATAGACTAGAGACATTAAGCACCAGAGACTTTATCTGCCCAAATTCTAAC
ATACCTGATCAAGAATCCTCTCTTCAGAGTTTTTGTAATTCTGAAAATAAGGTATTGAAA
GAAAATGCTGATTTTTTATCCCTGCGCCAGACTGAACTGCCAGGAAACTCTTGTGCTCAG
GATCCGGCATCCTTTATGCCTCCACAGCAGCCTTGCTCTTTCCCCAGCCAATCACTTTCA
GATGCTGAATCGATTTCTAAACATATGTCTTTGTCATATGTTGCTAATCAAGAGCCAGGT
ATTTTACAACAAAAAAATGCAGTTCAGATTATTAGTTCTGCTTTAGATACTGATAATGAA
TCTACAAAAGATACAGAAAATACTTTTGTCCTAGGAGATGTTCAAAAAACAGATGCCTTT
GTCCCAGTGTACTCTGACAGCACTATTCAAGAAGCATCACCAAACTTTGAGAAAGCTTAT
ACTTTACCTGTGTTACCATCAGAAAAGGACTTTAATGGAAGTGATGCCTCTACCCAGCTA
AATACACATTATGCATTTAGCAAACTAACTTACAAGTCTTCCAGTGGCCATGAAGTTGAG
AATAGCACAACTGATACTCAGGTCATTTCACATGAAAAAGAAAACAAACTGGAGAGTTTG
GTTTTAACTCATTTGAGTAGGTGTGATTCTGATTTATGTGAAATGAATGCAGGGATGCCA
AAAGGAAACCTAAATGAACAAGATCCAAAACATTGTCCTGAAAGTGAAAAGTGTTTGCTT
TCCATAGAAGATGAGGAATCTCAACAAAGCATTTTATCAAGTCTGGAAAACCATTCACAG
CAGTCAACTCAACCAGAAATGCATAAATATGGTCAGTTAGTTAAAGTAGAATTAGAAGAA
AATGCCGAAGATGATAAAACTGAAAACCAAATCCCTCAAAGAATGACTAGAAACAAAGCA
AATACAATGGCAAATCAAAGCAAACAGATTCTTGCTAGCTGTACACTATTATCAGAAAAA
GACAGTGAATCCTCATCTCCTAGAGGAAGAATAAGATTAACTGAAGATGACGATCCTCAA
ATTCACCATCCACGGAAAAGGAAAGTGTCACGTGTACCTCAGCCTGTGCAAGTGAGTCCC
TCTTTACTACAAGCAAAAGAGAAAACTCAGCAATCTCTGGCAGCCATTGTAGATTCTCTA
AAACTAGATGAGATTCAGCCATACAGTTCAGAGAGAGCAAATCCATATTTTGAATACTTG
CACATAAGGAAAAAAATAGAAGAAAAACGCAAATTACTGTGTAGTGTGATTCCTCAAGCA
CCTCAGTACTATGACGAATATGTAACATTTAACGGATCATATCTCCTGGATGGAAACCCC
TTAAGCAAGATTTGTATTCCCACAATTACACCACCACCTTCACTGTCAGATCCACTTAAA
GAGCTTTTTCGACAACAGGAAGTTGTAAGGATGAAACTACGTTTGCAACACAGTATTGAA
AGGGAAAAACTCATTGTATCCAACGAACAAGAAGTTTTGCGAGTTCATTACAGAGCTGCA
AGAACATTGGCAAATCAAACACTGCCATTTAGTGCTTGTACTGTTTTGCTGGATGCCGAA
GTATACAATGTACCATTGGACTCTCAGTCTGATGACAGTAAAACTTCTGTGAGGGATCGC
TTTAATGCAAGACAATTCATGTCTTGGTTACAAGATGTGGATGATAAATTTGACAAATTA
AAGACCTGTCTTTTAATGAGGCAACAACATGAAGCTGCGGCTTTAAATGCTGTCCAGAGG
TTAGAATGGCAGCTCAAACTCCAGGAACTTGATCCTGCCACCTATAAATCTATCAGCATT
TACGAAATCCAGGAGTTTTATGTTCCCCTTGTTGATGTTAACGACGACTTTGAATTGACT
CCTATA
Protein sequence predicted based on nucleotide sequence
MPKSGFTKPIQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIERSDVSKEMKEKSSMKR KLPFTISPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGNKKS TPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNK RNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAA GADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLL KREVPLSDDDESYTDSEEAQSVNPSSVDENIDSETEKDSLICESKQILPSKTPLPSALDE YEFKDDDDEEINKMIDDRHILRKEQRKENEPEAEKTHLFAKQEKAFYPKSFKSKKQKPSR VLYSSTESSDEEALQNKKISTSCSVIPETSNSDMQTKKEYVVSGEHKQKGKVKRKLKNQN KNKENQELKQEKEGKENTRITNLTVNTGLDCSEKTREEGNFRKSFSPKDDTSLHLFHIST GKSPKHSCGLSEKQSTPLKQEHTKTCLSPGSSEMSLQPDLVRYDNTESEFLPESSSVKSC KHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKER EKEKHKKEIEGEKEKYKTKDSAKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLTL EKKSKLEKNIKDDKSTKEKHVSKERNFKEERDKIKKESEKSFREEKIKDLKEERENIPTD KDSEFTSLGMSAIEESIGLHLVEKEIDIEKQEKHIKESKEKPEKRSQIKEKDIEKMERKT FEKEKKIKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKSKNTAAIKKTDDR EKSREKMDRKHDKEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDK KEKSRDKESINITNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDEPLKTPDGKEKDKK DKDIDRYKERDKHKDKIQINSLLKLKSEADKPKPKSSPASKDTRPKEKRLVNDDLMQTSF ERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKIKDKEKTKHTPTESKNKELTR SKSSEVTDAYTKEKQPKDAVSNRSQSVDTKNVMTLGKSSFVSDNSLNRSPRSENEKPGLS SRSVSMISVASSEDSCHTTVTTPRPPVEYDSDFMLESSESQMSFSQSPFLSIAKSPALHE RELDSLADLPERIKPPYANRLSTSHLRSSSVEDVKLIISEGRPTIEVRRCSMPSVICEHT KQFQTISEESNQGSLLTVPGDTSPSPKPEVFSNVPERDLSNVSNIHSSFATSPTGASNSK YVSADRNLIKNTAPVNTVMDSPVHLEPSSQVGVIQNKSWEMPVDRLETLSTRDFICPNSN IPDQESSLQSFCNSENKVLKENADFLSLRQTELPGNSCAQDPASFMPPQQPCSFPSQSLS DAESISKHMSLSYVANQEPGILQQKNAVQIISSALDTDNESTKDTENTFVLGDVQKTDAF VPVYSDSTIQEASPNFEKAYTLPVLPSEKDFNGSDASTQLNTHYAFSKLTYKSSSGHEVE NSTTDTQVISHEKENKLESLVLTHLSRCDSDLCEMNAGMPKGNLNEQDPKHCPESEKCLL SIEDEESQQSILSSLENHSQQSTQPEMHKYGQLVKVELEENAEDDKTENQIPQRMTRNKA NTMANQSKQILASCTLLSEKDSESSSPRGRIRLTEDDDPQIHHPRKRKVSRVPQPVQVSP SLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQA PQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIE REKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNVPLDSQSDDSKTSVRDR FNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISI YEIQEFYVPLVDVNDDFELTPI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
