Gene:
Gene Symbol:
SDK2
Gene Name:
sidekick cell adhesion molecule 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069256)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00353990
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAAGGTGGTGGACATCCCCTGTCAGGCCAAAGGTGTGCCGCCGCCCTCCATCACC
TGGTACAAGGACGCAGCCGTGGTGGAGGTGGAGAAGTTGACCCGCTTCCGGCAGCGCAAC
GACGGGGGCCTGCAGATCAGCGGCCTGGTGCCCGATGATACCGGCATGTTCCAGTGCTTC
GCCCGCAATGCAGCCGGCGAGGTGCAAACTTCCACCTACCTGGCTGTCACCAGCATCGCC
CCTAACATCACCAGAGGCCCCCTGGACAGCACGGTGATCGATGGCATGTCAGTGGTGCTA
GCATGTGAGACCTCGGGGGCGCCCCGACCAGCTATCACTTGGCAGAAAGGGGAGCGCATC
TTGGCCAGTGGCTCTGTGCAGCTGCCTCGCTTCACACCCCTGGAGTCGGGCAGCCTCCTC
ATCAGCCCCACACACATCTCCGATGCGGGGACCTACACCTGCCTGGCCACCAACTCTCGG
GGGGTCGATGAGGCCTCAGCAGACCTAGTCGTTTGGGCTCGGACCCGCATCACCAAGCCC
CCCCAGGATCAGAGTGTCATCAAGGGCACCCAGGCCTCCATGGTGTGCGGAGTGACCCAC
GACCCCCGAGTAACCATCAGGTACATCTGGGAGAAGGACGGGGCCACCCTGGGCACGGAG
AGCCATCCTCGTATCCGCCTGGACAGAAACGGCTCCCTGCACATCTCACAGACGTGGTCG
GGAGACATCGGCACGTACACCTGCCGGGTGATCTCAGCAGGAGGCAACGACTCTCGCAGT
GCCCACCTGCGAGTCAGGCAACTGCCCCACGCGCCCGAGCACCCAGTGGCCACTCTCAGC
ACCGTGGAAAGGCGAGCCATCAACCTGACGTGGACCAAGCCCTTTGATGGCAACAGCCCC
CTGATCCGCTACATTCTGGAGATGTCGGAGAACAATGCCCCCTGGACTGTACTCCTGGCC
AGTGTGGACCCCAAAGCTACCTCAGTGACAGTCAAGGGCCTGGTTCCTGCACGCTCCTAC
CAGTTCCGTCTTTGTGCCGTCAACGACGTGGGGAAAGGACAGTTCAGCAAAGACACCGAG
AGGGTCTCCCTCCCCGAGGAGCCCCCCACGGCCCCTCCACAGAACGTCATCGCCAGCGGT
CGAACCAACCAGTCCATCATGATCCAGTGGCAGCCGCCTCCTGAGAGCCACCAGAATGGA
ATTCTCAAGGGTTACATCATCAGGTACTGCCTGGCCGGGCTGCCCGTGGGGTACCAGTTT
AAGAACATCACGGATGCTGATGTGAACAACCTGCTGCTGGAGGATCTCATCATTTGGACC
AACTACGAGATCGAGGTGGCTGCTTACAACAGCGCTGGGCTGGGGGTCTACAGCAGTAAA
GTCACCGAGTGGACGCTGCAGGGAGTTCCCACAGTCCCTCCGGGCAATGTGCACGCGGAA
GCCACCAATTCCACGACCATCCGCTTCACCTGGAACGCCCCCAGCCCCCAGTTCATCAAC
GGCATCAACCAGGGCTACAAGCTGATCGCCTGGGAGCCGGAACAGGAAGAGGAGGTTACC
ATGGTGACCGCCCGGCCTAACTTTCAAGACAGCATCCACGTGGGCTTCGTGTCTGGCCTG
AAGAAGTTCACCGAGTACTTCACCTCAGTGCTGTGTTTCACCACCCCCGGGGACGGGCCA
CGCAGCACCCCGCAGCTGGTGCGCACCCATGAGGATGTGCCTGGGCCCGTGGGACACCTG
AGCTTCAGTGAGATCCTGGACACATCGCTGAAGGTCAGCTGGCAAGAGCCGGGAGAGAAA
AATGGCATCCTCACAGGGTACCGGATCTCCTGGGAGGAGTACAATCGAACCAACACCCGT
GTGACCCACTACCTGCCCAACGTGACCCTGGAGTACCGTGTCACGGGCCTCACCGCGCTC
ACCACCTACACCATCGAGGTGGCCGCCATGACCTCAAAGGGCCAGGGCCAAGTGTCCGCC
TCCACCATCTCCTCTGGGGTGCCCCCAGAACTCCCAGGGCCCCCCACCAACCTGGGCATT
TCCAACATCGGCCCCCGCTCTGTGACCTTGCAGTTCAGGCCAGGCTACGATGGGAAAACC
TCCATCTCCCGCTGGCTGGTGGAAGCCCAGGTAGGCGTGGTTGGGGAGGGAGAGGAGTGG
TTGCTGATCCACCAGCTCTCCAATGAGCCCGATGCCCGCTCCATGGAGGTGCCCGACCTC
AACCCCTTCACCTGCTACAGCTTCCGCATGCGCCAGGTGAACATCGTGGGCACCAGCCCC
CCCAGTCAGCCTTCTAGAAAGATCCAGACCCTGCAGGCACCCCCTGACATGGCCCCAGCC
AATGTGTCTCTGCGCACAGCCAGTGAGACCAGCCTGTGGCTGCGCTGGATGCCTCTCCCG
GAGATGGAATACAATGGGAACCCTGAGTCCGTGGGCTATAAGATCAAGTACAGCCGGTCA
GACGGGCATGGCAAGACGCTGAGCCACGTGGTGCAGGACCGTGTGGAGCGGGACTACACC
ATCGAGGACCTGGAGGAGTGGACAGAGTACCGCGTCCAGGTCCAGGCCTTCAACGCCATC
GGGAGCGGGCCCTGGAGCCAGACGGTGGTGGGCAGGACCCGGGAGTCAGTTCCCTCTTCC
GGCCCCACCAATGTGTCTGCACTGGCCACCACCTCCAGCAGCATGCTGGTGCGCTGGAGC
GAGGTCCCCGAGGCTGATCGCAACGGGCTCGTGCTGGGCTATAAGGTGATGTATAAGGAG
AAGGACTCGGACACCCAGCCCCGATTCTGGCTGGTGGAAGGCAACTCGTCTCGCAGTGCC
CAGCTCACCGGCTTGGGCAAATACGTGCTCTATGAAGTCCAGGTGCTGGCCTTCACACGC
ATCGGGGACGGCAGCCCCAGCCACCCTCCCATCCTGGAGCGGACGCTGGATGATGTCCCA
GGACCACCCATGGGCATCCTGTTCCCAGAGGTGCGGACCACGTCTGTGCGGCTGATCTGG
CAGCCCCCTGCCGCCCCCAATGGCATCATTCTTGCTTACCAGATCACACACCGGCTCAAC
ACCACCACGGCCAACACCGCCACTGTGGAGGTGCTGGCACCCAGCGCCCGGCAGTACACA
GCCACGGGCCTCAAGCCAGAGTCTGTCTACCTGTTCCGCATCACGGCCCAGACCCGCAAG
GGCTGGGGAGAAGCTGCCGAGGCCTTGGTGGTGACCACCGAGAAGAGAGACCGTCCGCAG
CCCCCCAGCAGGCCGATGGTGCAGCAGGAGGATGTGAGAGCACGCAGCGTGCTGCTGTCC
TGGGAGCCAGGGAGCGACGGGCTCTCCCCTGTGCGCTACTACACCATCCAGACCCGCGAG
CTGCCCAGCGGCAGGTGGGCACTGCACTCGGCCTCCGTGAGCCACAATGCCTCCAGCTTC
ATTGTGGACAGGCTGAAGCCCTTCACGTCCTACAAGTTCCGAGTGAAGGCGACCAATGAC
ATTGGCGACAGCGAGTTCAGCGAGGAGTCGGAGTCACTGACCACCCTGCAGGCTGCCCCC
GATGAAGCACCCACCATCCTCTCCGTGACGCCCCACACCACCACCTCCGTGCTAATCCGA
TGGCAGCCGCCAGCAGAGGACAAGATCAATGGCATCCTCCTGGGCTTCCGGATCCGATAC
CGGGAGCTGCTCTATGAAGGACTGAGGGGCTTCACGCTTCGAGGCATCAACAACCCAGGG
GCCACATGGGCTGAGCTTACCTCCATGTACTCCATGCGGAACCTGAGCCGGCCCAGCCTC
ACGCAGTACGAGCTGGACAACCTGAACAAGCACAGGCGGTACGAGATACGGATGAGCGTG
TACAACGCTGTGGGTGAGGGGCCCTCCAGCCCCCCGCAGGAGGTCTTTGTTGGGGAGGCA
GTGCCCACAGCAGCACCTCGTAACGTGGTCGTCCACGGCGCCACGGCCACACAGCTGGAC
GTGACTTGGGAGCCACCTCCGCTGGACAGCCAGAATGGAGACATCCAGGGGTACAAGATT
TATTTCTGGGAAGCCCAGCGGGGGAACCTCACAGAGCGAGTGAAGACGCTTTTCCTGGCT
GAGAACAGCGTGAAGCTCAAGAACTTGACTGGCTACACGGCCTACATGGTCAGCGTGGCC
GCCTTCAACGCCGCTGGGGATGGGCCTCGGAGCACCCCCACCCAAGGCCAGACCCAGCAA
GCAGCCCCCAGCGCTCCCAGCTCGGTCAAGTTCAGTGAGCTGACCACAACCTCAGTGAAT
GTGTCCTGGGAAGCCCCGCAGTTCCCCAATGGCATCCTGGAGGGCTACAGGCTGGTGTAC
GAGCCCTGCAGCCCCGTGGATGGAGTCAGCAAGATCGTGACCGTGGACGTGAAGGGGAAC
AGCCCCCTGTGGCTGAAGGTGAAGGACCTGGCGGAGGGGGTGACCTACAGGTTCCGCATC
AGAGCCAAGACCTTCACCTACGGGCCGGAGATCGAAGCCAACGTCACCACGGGCCCCGGA
GAAGGTGCCCCAGGACCGCCTGGCGTGCCCATCATCGTGCGGTACAGCTCTGCCATCGCC
ATTCACTGGTCCAGCGGAGACCCGGGCAAAGGGCCCATCACCCGCTACGTCATCGAGGCC
AGACCTTCAGACGAGGGACTATGGGACATCCTCATCAAAGACATCCCCAAGGAGGTGAGC
TCCTACACGTTCAGCATGGACATCCTGAAGCCGGGCGTGAGCTATGACTTCCGGGTCATC
GCGGTCAACGACTATGGTTTCGGCACCCCCAGCAGCCCCTCCCAGTCTGTGCCAGCCCAG
AAAGCCAACCCCTTCTATGAGGAGTGGTGGTTCTTGGTGGTCATTGCCCTGGTCGGCCTC
ATCTTCATCCTGCTTCTGGTCTTCGTGCTCATCATCCGGGGCCAGAGCAAGAAGTACGCC
AAGAAGACAGACTCGGGGAACAGTGCCAAGTCTGGAGCCCTAGGCCACAGCGAGATGATG
AGCTTGGATGAAAGCAGCTTCCCTGCCCTGGAACTCAACAACAGGCGGCTCTCCGTCAAG
AACTCTTTCTGCCGAAAGAACGGCCTGTACACCAGGTCTCCCCCCAGGCCCAGCCCAGGC
AGCCTGCACTACTCGGATGAGGATGTCACCAAATACAACGACCTCATCCCTGCAGAGAGC
AGCAGCCTGACGGAGAAGCCCTCAGAAATCTCCGACTCTCAGGGAAGTGACAGCGAGTAC
GAGGTCGACTCAAACCACCAGAAGGCCCACTCCTTTGTCAACCACTACATCAGTGACCCC
ACATACTACAACTCGTGGCGGCGACAGCAGAAGGGCATCTCGAGGGCACAGGCCTACAGC
TACACGGAGAGCGACTCGGGTGAGCCAGACCACACCACCGTCACCAACAGCACCAGCACC
CAGCAGGGCAGCCTCTTTCGGCCCAAGGCCAGTCGGACTCCAACGCCCCAGAACCCCCCT
AACCCCCCAAGTCAGCAGAGCACCCTCTACCGTCCCCCCAGCAGCCTGGCCCCAGGCTCC
CGGGCTCCCATAGCAGGATTTTCATCATTTGTTTAA
Protein sequence predicted based on nucleotide sequence
MEKVVDIPCQAKGVPPPSITWYKDAAVVEVEKLTRFRQRNDGGLQISGLVPDDTGMFQCF ARNAAGEVQTSTYLAVTSIAPNITRGPLDSTVIDGMSVVLACETSGAPRPAITWQKGERI LASGSVQLPRFTPLESGSLLISPTHISDAGTYTCLATNSRGVDEASADLVVWARTRITKP PQDQSVIKGTQASMVCGVTHDPRVTIRYIWEKDGATLGTESHPRIRLDRNGSLHISQTWS GDIGTYTCRVISAGGNDSRSAHLRVRQLPHAPEHPVATLSTVERRAINLTWTKPFDGNSP LIRYILEMSENNAPWTVLLASVDPKATSVTVKGLVPARSYQFRLCAVNDVGKGQFSKDTE RVSLPEEPPTAPPQNVIASGRTNQSIMIQWQPPPESHQNGILKGYIIRYCLAGLPVGYQF KNITDADVNNLLLEDLIIWTNYEIEVAAYNSAGLGVYSSKVTEWTLQGVPTVPPGNVHAE ATNSTTIRFTWNAPSPQFINGINQGYKLIAWEPEQEEEVTMVTARPNFQDSIHVGFVSGL KKFTEYFTSVLCFTTPGDGPRSTPQLVRTHEDVPGPVGHLSFSEILDTSLKVSWQEPGEK NGILTGYRISWEEYNRTNTRVTHYLPNVTLEYRVTGLTALTTYTIEVAAMTSKGQGQVSA STISSGVPPELPGPPTNLGISNIGPRSVTLQFRPGYDGKTSISRWLVEAQVGVVGEGEEW LLIHQLSNEPDARSMEVPDLNPFTCYSFRMRQVNIVGTSPPSQPSRKIQTLQAPPDMAPA NVSLRTASETSLWLRWMPLPEMEYNGNPESVGYKIKYSRSDGHGKTLSHVVQDRVERDYT IEDLEEWTEYRVQVQAFNAIGSGPWSQTVVGRTRESVPSSGPTNVSALATTSSSMLVRWS EVPEADRNGLVLGYKVMYKEKDSDTQPRFWLVEGNSSRSAQLTGLGKYVLYEVQVLAFTR IGDGSPSHPPILERTLDDVPGPPMGILFPEVRTTSVRLIWQPPAAPNGIILAYQITHRLN TTTANTATVEVLAPSARQYTATGLKPESVYLFRITAQTRKGWGEAAEALVVTTEKRDRPQ PPSRPMVQQEDVRARSVLLSWEPGSDGLSPVRYYTIQTRELPSGRWALHSASVSHNASSF IVDRLKPFTSYKFRVKATNDIGDSEFSEESESLTTLQAAPDEAPTILSVTPHTTTSVLIR WQPPAEDKINGILLGFRIRYRELLYEGLRGFTLRGINNPGATWAELTSMYSMRNLSRPSL TQYELDNLNKHRRYEIRMSVYNAVGEGPSSPPQEVFVGEAVPTAAPRNVVVHGATATQLD VTWEPPPLDSQNGDIQGYKIYFWEAQRGNLTERVKTLFLAENSVKLKNLTGYTAYMVSVA AFNAAGDGPRSTPTQGQTQQAAPSAPSSVKFSELTTTSVNVSWEAPQFPNGILEGYRLVY EPCSPVDGVSKIVTVDVKGNSPLWLKVKDLAEGVTYRFRIRAKTFTYGPEIEANVTTGPG EGAPGPPGVPIIVRYSSAIAIHWSSGDPGKGPITRYVIEARPSDEGLWDILIKDIPKEVS SYTFSMDILKPGVSYDFRVIAVNDYGFGTPSSPSQSVPAQKANPFYEEWWFLVVIALVGL IFILLLVFVLIIRGQSKKYAKKTDSGNSAKSGALGHSEMMSLDESSFPALELNNRRLSVK NSFCRKNGLYTRSPPRPSPGSLHYSDEDVTKYNDLIPAESSSLTEKPSEISDSQGSDSEY EVDSNHQKAHSFVNHYISDPTYYNSWRRQQKGISRAQAYSYTESDSGEPDHTTVTNSTST QQGSLFRPKASRTPTPQNPPNPPSQQSTLYRPPSSLAPGSRAPIAGFSSFV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
