Gene:
Gene Symbol:
OTUD7A
Gene Name:
OTU domain containing 7A
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069276)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00354010
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGTTTCTAGTGTGCTTCCAAACCCCACCTCGGCTGAGTGTTGGGCAGCACTTCTACAT
GATCCTATGACTCTTGATATGGACGCAGTCCTGTCAGACTTTGTTCGGTCCACGGGGGCA
GAACCTGGTCTGGCCAGAGACCTGCTGGAAGGCAAAAACTGGGACCTGACAGCCGCTCTG
AGCGACTATGAGCAGCTCCGCCAGGTGCACACAGCCAATCTGCCACATGTGTTCAATGAA
GGGCGGGGTCCCAAGCAGCCAGAGCGAGAGCCACAGCCCGGGCACAAGGTGGAGCGACCC
TGCCTGCAGAGGCAGGACGACATTGCCCAAGAAAAGCGGCTTTCCCGGGGGATTTCCCAC
GCCAGCTCAGCCATCGTCTCCCTGGCCCGGTCCCACGTGGCAAGTGAATGCAACAACGAG
CAGTTCCCCCTGGAGATGCCAATCTACACATTCCAGTTGCCAGACCTGAGCGTGTACAGC
GAGGATTTCAGGAGCTTCATCGAGCGGGACTTGATCGAGCAGGCAACAATGGTGGCTTTG
GAGCAGGCAGGTCGCCTGAACTGGTGGTCCACTGTGTGCACGAGCTGTAAACGGCTTCTT
CCTCTGGCCACAACAGGGGATGGGAACTGCCTTTTACATGCTGCTTCACTGGGAATGTGG
GGGTTTCACGACCGGGACCTGGTGTTACGGAAAGCTCTCTATACCATGATGAGGACGGGA
GCTGAGAGGGAAGCCCTGAAGCGGAGGTGGAGGTGGCAGCAGACGCAGCAGAATAAGGAG
GAGGAGTGGGAGCGGGAGTGGACGGAGCTGCTGAAGCTGGCCTCCAGCGAGCCGCGCACA
CACTTCAGCAAGAATGGCGGCACGGGCGGGGGTGTGGACAACTCTGAGGACCCCGTGTAC
GAGAGCCTGGAAGAGTTCCACGTTTTTGTCCTAGCCCATATATTAAGAAGGCCCATCGTT
GTTGTGGCAGATACAATGTTAAGAGACTCAGGTGGAGAAGCGTTCGCACCCATCCCATTC
GGAGGGATCTACCTGCCCTTGGAGGTCCCTCCCAACAGATGCCACTGCTCGCCTCTGGTT
CTGGCCTATGATCAAGCCCATTTCTCTGCCCTTGTGTCCATGGAACAGAGAGACCAGCAA
AGAGAACAAGCCGTGATCCCCCTGACGGATTCTGAGCACAAGCTGCTGCCTCTGCACTTT
GCAGTGGACCCTGGCAAGGACTGGGAGTGGGGGAAAGACGACAACGATAACGCCCGGCTG
GCCCACCTTATCCTGTCGCTAGAAGCCAAGCTGAACCTTCTGCACAGCTACATGAACGTG
ACGTGGATCCGGATCCCCTCCGAGACACGGGCGCCCCTGGCACAGCCGGAGTCTCCCACG
GCCTCGGCAGGGGAGGACGTGCAGTCCCTGGCCGACTCGCTGGACTCGGACCGCGATTCG
GTGTGCAGCAATTCTAACAGCAATAACGGCAAGAACGGCAAGGACAAGGAGAAGGAGAAG
CAGCGCAAGGAGAAGGACAAGACGCGCGCCGACTCCGTGGCCAACAAGCTGGGCAGCTTC
AGCAAGACGCTGGGCATCAAGCTGAAGAAAAACATGGGCGGCCTCGGCGGCCTGGTGCAC
GGCAAGATGGGCCGCGCCAACTCCGCCAATGGCAAGAACGGGGACTCGGCCGAGCGGGGC
AAGGAGAAGAAGGCCAAGTCGCGCAAGGGCAGCAAGGAGGAGTCTGGTGCGTCGGCCAGC
ACGTCGCCGTCGGAAAAGACCACGCCGTCGCCCACAGACAAGGCAGCGGGCGCGTCGCCG
GCGGAGAAGGGCGGTGGGCCGCGGGGCGACGCCTGGAAGTACAGCACGGATGTGAAGCTG
AGCCTCAACATCCTGCGCGCCGCCATGCAGGGGGAGCGCAAGTTCATCTTCGCCGGCCTG
CTGCTCACCAGCCACCGGCACCAGTTCCACGAGGAGATGATCGGCTACTACCTGACGAGC
GCGCAGGAGCGCTTCAGCGCCGAGCAGGAGCAGCGGCGCCGCGACGCCGCTACTGCGGCC
GCCGCTGCCGCCGCCGCCGCCGCCGCCACGGCCAAGCGGCCGCCGCGCAGACCGGAGACG
GAGGGCGTGCCGGTCCCGGAGCGCGCCTCTCCGGGCCCACCCACGCAGCTGGTGCTCAAG
CTCAAGGAGCGGCCGAGCCCCGGGCCCGCGGCAGGGCGTGCGGCGCGGGCGGCGGCGGGC
GGCACGGCCTCCCCGGGGGGAGGCGCGCGGCGTGCGAGCGCCAGCGGACCAGTGCCTGGC
CGCAGCCCCCCGGCGCCAGCGCGCCAGAGCGTCATCCACGTGCAGGCGTCGGGCGCGCGG
GACGAGGCGTGCGCGCCGGCCGTGGGGGCGCTGCGGCCGTGCGCCACGTACCCGCAGCAG
AACCGCTCGCTGTCGTCGCAGAGCTACAGCCCGGCGCGCGCCGCCGCCCTGCGCACCGTC
AACACGGTCGAGTCGCTGGCGCGCGCGGTGCCCGGGGCCCTACCGGGCGCGGCGGGGACG
GCGGGGGCGGCCGAGCACAAGTCGCAGACCTACACCAACGGCTTCGGCGCCCTGCGCGAC
GGCCTGGAGTTCGCCGACGCCGACGCGCCGACCGCGCGCTCGAACGGTGAGTGCGGCCGT
GGCGGCCCGGGGCCGGTGCAGCGGCGCTGCCAGCGCGAGAACTGTGCGTTCTACGGGCGC
GCCGAGACCGAGCACTACTGCTCCTACTGCTACCGCGAGGAGCTGCGGCGGCGGCGCGAG
GCGCGCGGGGCCCGGCCC
Protein sequence predicted based on nucleotide sequence
MVSSVLPNPTSAECWAALLHDPMTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDLTAAL SDYEQLRQVHTANLPHVFNEGRGPKQPEREPQPGHKVERPCLQRQDDIAQEKRLSRGISH ASSAIVSLARSHVASECNNEQFPLEMPIYTFQLPDLSVYSEDFRSFIERDLIEQATMVAL EQAGRLNWWSTVCTSCKRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYTMMRTG AEREALKRRWRWQQTQQNKEEEWEREWTELLKLASSEPRTHFSKNGGTGGGVDNSEDPVY ESLEEFHVFVLAHILRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPPNRCHCSPLV LAYDQAHFSALVSMEQRDQQREQAVIPLTDSEHKLLPLHFAVDPGKDWEWGKDDNDNARL AHLILSLEAKLNLLHSYMNVTWIRIPSETRAPLAQPESPTASAGEDVQSLADSLDSDRDS VCSNSNSNNGKNGKDKEKEKQRKEKDKTRADSVANKLGSFSKTLGIKLKKNMGGLGGLVH GKMGRANSANGKNGDSAERGKEKKAKSRKGSKEESGASASTSPSEKTTPSPTDKAAGASP AEKGGGPRGDAWKYSTDVKLSLNILRAAMQGERKFIFAGLLLTSHRHQFHEEMIGYYLTS AQERFSAEQEQRRRDAATAAAAAAAAAAATAKRPPRRPETEGVPVPERASPGPPTQLVLK LKERPSPGPAAGRAARAAAGGTASPGGGARRASASGPVPGRSPPAPARQSVIHVQASGAR DEACAPAVGALRPCATYPQQNRSLSSQSYSPARAAALRTVNTVESLARAVPGALPGAAGT AGAAEHKSQTYTNGFGALRDGLEFADADAPTARSNGECGRGGPGPVQRRCQRENCAFYGR AETEHYCSYCYREELRRRREARGARP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
