Original Clone ID:
NCI10412
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
HsCD00358149
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG
GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA
GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT
CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT
GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG
GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC
TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA
ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT
ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT
TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG
AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG
GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA
TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC
TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG
GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG
Protein sequence predicted based on nucleotide sequence
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRH PNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCH SRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSAR YSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNT FPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
Gene
Gene Symbol :
CDK1
Symbol Nomenclature :
CDK1
SYNONYM :
CDC2; CDC28A; P34CDC2
Designation :
cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
Full Nomenclature :
cyclin-dependent kinase 1
GENEID :
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Reference Sequence Alignment
HsCD00358149 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG XM_005270303.1 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG NM_001786.4 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG ************************************************************ HsCD00358149 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA XM_005270303.1 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA NM_001786.4 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA ************************************************************ HsCD00358149 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT XM_005270303.1 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT NM_001786.4 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT ************************************************************ HsCD00358149 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT XM_005270303.1 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT NM_001786.4 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT ************************************************************ HsCD00358149 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG XM_005270303.1 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG NM_001786.4 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG ************************************************************ HsCD00358149 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC XM_005270303.1 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC NM_001786.4 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC ************************************************************ HsCD00358149 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA XM_005270303.1 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA NM_001786.4 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA ************************************************************ HsCD00358149 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT XM_005270303.1 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT NM_001786.4 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT ************************************************************ HsCD00358149 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT XM_005270303.1 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT NM_001786.4 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT ************************************************************ HsCD00358149 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG XM_005270303.1 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG NM_001786.4 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG ************************************************************ HsCD00358149 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG XM_005270303.1 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG NM_001786.4 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG ************************************************************ HsCD00358149 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA XM_005270303.1 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA NM_001786.4 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA ************************************************************ HsCD00358149 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC XM_005270303.1 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC NM_001786.4 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC ************************************************************ HsCD00358149 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG XM_005270303.1 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG NM_001786.4 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG ************************************************************ HsCD00358149 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG XM_005270303.1 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG NM_001786.4 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG ******************************************************
Pathways
NCI :
AP-1 transcription factor network
NCI :
E2F transcription factor network
NCI :
FOXM1 transcription factor network
NCI :
p73 transcription factor network
NCI :
PLK1 signaling events
NCI :
Retinoic acid receptors-mediated signaling
Reactome :
Activated TLR4 signalling
Reactome :
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
Activation of NIMA Kinases NEK9, NEK6, NEK7
Reactome :
APC/C-mediated degradation of cell cycle proteins
Reactome :
APC/C:Cdc20 mediated degradation of Cyclin B
Reactome :
APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome :
Cell Cycle
Reactome :
Cell Cycle Checkpoints
Reactome :
Cell Cycle, Mitotic
Reactome :
Centrosome maturation
Reactome :
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
Reactome :
Condensation of Prometaphase Chromosomes
Reactome :
Condensation of Prophase Chromosomes
Reactome :
Cyclin A/B1 associated events during G2/M transition
Reactome :
Cyclin B2 mediated events
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Depolymerisation of the Nuclear Lamina
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
E2F mediated regulation of DNA replication
Reactome :
E2F-enabled inhibition of pre-replication complex formation
Reactome :
ERK activation
Reactome :
ERK1 activation
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
G0 and Early G1
Reactome :
G1/S Transition
Reactome :
G1/S-Specific Transcription
Reactome :
G2/M Checkpoints
Reactome :
G2/M DNA damage checkpoint
Reactome :
G2/M DNA replication checkpoint
Reactome :
G2/M Transition
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
Golgi Cisternae Pericentriolar Stack Reorganization
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin-2 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
Loss of Nlp from mitotic centrosomes
Reactome :
Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome :
M Phase
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MASTL Facilitates Mitotic Progression
Reactome :
Mitotic G1-G1/S phases
Reactome :
Mitotic G2-G2/M phases
Reactome :
Mitotic M-M/G1 phases
Reactome :
Mitotic Prometaphase
Reactome :
Mitotic Prophase
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Nuclear Envelope Breakdown
Reactome :
Nuclear Pore Complex (NPC) Disassembly
Reactome :
Phosphorylation of Emi1
Reactome :
Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes
Reactome :
Phosphorylation of the APC/C
Reactome :
Prolonged ERK activation events
Reactome :
RAF/MAP kinase cascade
Reactome :
Recruitment of mitotic centrosome proteins and complexes
Reactome :
Recruitment of NuMA to mitotic centrosomes
Reactome :
Regulation of APC/C activators between G1/S and early anaphase
Reactome :
Regulation of mitotic cell cycle
Reactome :
Regulation of PLK1 Activity at G2/M Transition
Reactome :
Resolution of Sister Chromatid Cohesion
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Cell cycle
WikiPathway :
DNA damage response
WikiPathway :
G1 to S cell cycle control
WikiPathway :
Integrated Cancer pathway
WikiPathway :
miRNA regulation of DNA Damage Response
WikiPathway :
TGF beta Signaling Pathway
Protein
SMART domain :
SMART domain :
UniProt :
B7Z3D6
UniProt :
HPRD :
00302
Clone
Organism
Species Specific ID:
Genome
Reagents
Labome :