LCTL (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  LCTL
Gene Name:  lactase-like
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358266
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1545nts
Open reading frame: 1 to 1545

CCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGCTTCTCCTGGGGCGTGGGC
AGTTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGGAAAGGGCCTAGCATCTGG
GACGTCTTCACACACAGTGGGAAGGGGAAAGTGCTTGGGAATGAGACGGCAGATGTAGCC
TGTGACGGCTACTACAAGGTCCAGGAGGACATCATTCTGCTGAGGGAACTGCACGTCAAC
CACTACCGATTCTCCCTGTCTTGGCCCCGGCTCCTGCCCACAGGCATCCGAGCCGAGCAG
GTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGATGCCCTTCTGAGCAGCAAC
ATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAGCTGCTCCAGGTCAAATAC
GGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGACTACGCCAACCTGTGCTTT
GAGGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGTGATCCTCGGGCAATGGCA
GAAAAAGGCTATGAGACGGGCCACCATGCGCCGGGCCTGAAGCTCCGCGGCACCGGCCTG
TACAAGGCAGCACACCACATCATTAAGGCCCACGCCAAAGCCTGGCATTCTTATAACACC
ACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGGAATTTCATTGAACTGTGACTGGGGGGAA
CCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAGAGATACCTACAGTTCTGT
CTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCCCAAGTCATGAAGGACTAC
ATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGGTTACCGGTGTTCTCACTC
CAGGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGATTAGGTCATTTTACTACT
CGGTACATCACGGAAAGGAACTACCCCTCCCGCCAGGGGCCCAGCTACCAGAACGATCGT
GACTTGATAGAGCTGGTTGACCCAAACTGGCCAGATCTGGGGTCTAAATGGCTATATTCT
GTGCCATGGGGATTTAGGAGGCTCCTTAACTTTGCTCAGACTCAATACGGTGATCCTCCC
ATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGTACTCAATTATGTGATGAG
TGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTAAAAGCTATAAAAGATGGT
GCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAGTTTGAATGGGAGAAAGGA
TACTCAGATAGATATGGATTCTACTATGTTGAATTTAACGACAGAAATAAGCCTCGCTAT
CCAAAGGCTTCAGTTCAATATTACAAGAAGATTATCATTGCCAATGGGTTTCCCAATCCA
AGAGAGGTGGAAAGTTGGTACCTCAAAGCTTTGGAAACTTGCTCTATCAACAATCAGATG
CTTGCTGCAGAGCCCTTGCTAAGTCACATGCAAATGGTTACGGAG

Protein sequence predicted based on nucleotide sequence

PEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVA
CDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSN
ITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMA
EKGYETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGE
PVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSL
QEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYS
VPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDG
ANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNP
REVESWYLKALETCSINNQMLAAEPLLSHMQMVTE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,78; del@1627,75

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable