HYAL4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  HYAL4
Gene Name:  hyaluronoglucosaminidase 4
Original Clone ID:
None
Keyword:
partial cds (N and C terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358291
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1275nts
Open reading frame: 1 to 1275

AAGTCTATCTCTTGTCTAAAACCTGCTCGACTTCCAATTTATCAAAGGAAACCTTTTATA
GCTGCTTGGAATGCTCCAACAGATCAGTGTTTGATAAAATATAATTTAAGACTAAATTTG
AAAATGTTTCCTGTGATTGGAAGCCCACTGGCCAAGGCCAGGGGGCAAAATGTCACTATA
TTTTATGTCAACAGATTGGGATACTATCCGTGGTATACATCACAAGGGGTCCCCATTAAT
GGAGGTCTCCCACAGAACATAAGTTTACAAGTACATCTGGAAAAAGCTGACCAAGATATT
AATTATTACATCCCTGCTGAAGATTTCAGTGGACTTGCTGTTATAGATTGGGAATATTGG
CGACCACAGTGGGCCCGGAACTGGAACTCAAAAGATGTTTACAGACAGAAGTCAAGAAAG
CTTATTTCCGATATGGGAAAGAATGTATCAGCTACCGATATTGAATATTTAGCCAAAGTG
ACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAGGAAACCATCAAATTGGGAATTAAGAGC
CGACCCAAAGGCCTTTGGGGTTATTATTTATATCCTGATTGCCACAATTATAACGTTTAT
GCCCCAAACTACTCTGGGTCATGCCCAGAAGACGAAGTCTTGAGGAACAATGAGCTCTCT
TGGCTCTGGAACAGCAGTGCTGCTTTATATCCTTCTATCGGTGTCTGGAAATCCCTTGGA
GACAGTGAAAACATTTTGCGCTTCTCCAAATTTCGGGTGCATGAATCCATGAGGATCTCC
ACCATGACATCTCATGATTATGCTCTGCCTGTATTTGTCTACACAAGGCTAGGGTACAGA
GATGAACCTTTATTTTTCCTTTCTAAGCAAGATCTAGTCAGCACCATAGGAGAAAGTGCT
GCCTTGGGAGCTGCAGGCATTGTTATTTGGGGAGACATGAATTTAACTTCATCCAAGGCC
AACTGTACAAAGGTGAAGCAGTTTGTGAGTTCTGATTTAGGGAGCTACATAGCCAATGTG
ACCAGAGCTGCTGAGGTATGCAGCCTTCACCTCTGCAGGAACAATGGCAGGTGCATAAGG
AAGATGTGGAACGCGCCCAGTTACCTTCACTTGAACCCTGCAAGTTACCACATAGAGGCC
TCTGAGGACGGGGAGTTTACTGTGAAAGGAAAAGCATCTGATACAGACCTGGCAGTGATG
GCAGATACATTTTCCTGTCATTGTTATCAGGGATATGAAGGAGCTGATTGCAGAGAAATA
AAGACGGCTGATTAG

Protein sequence predicted based on nucleotide sequence

KSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTI
FYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYW
RPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKS
RPKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLG
DSENILRFSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESA
ALGAAGIVIWGDMNLTSSKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIR
KMWNAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREI
KTAD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,87; del@1363,84

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable