B3GALT4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  B3GALT4
Gene Name:  UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358341
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1059nts
Open reading frame: 1 to 1059

TCGGGGTTGGGGGAGGAGCTGCTGAGCCTCTCACTAGCCTCCCTGCTCCCAGCCCCCGCC
TCACCGGGGCCGCCCCTGGCCCTGCCCCGCCTCTTGATCCCCAACCAGGAAGCTTGCAGT
GGTCCCGGGGCCCCTCCCTTCCTGCTCATCCTGGTGTGCACGGCTCCGGAGAACCTGAAC
CAGAGAAACGCCATTCGGGCTTCGTGGGGCGGGCTGCGCGAGGCCCGGGGGCTCAGGGTA
CAGACGCTATTCTTGCTGGGAGAGCCGAACGCACAGCACCCCGTGTGGGGTTCCCAGGGG
AGTGACCTGGCCTCGGAGTCAGCAGCCCAGGGGGATATCTTGCAGGCCGCCTTCCAGGAC
TCCTACCGCAACCTCACCCTAAAGACCCTCAGCGGGCTGAACTGGGCTGAGAAACACTGC
CCCATGGCCCGATACGTCCTCAAGACGGACGATGATGTGTATGTCAACGTCCCTGAACTG
GTATCAGAGCTGGTCTTGCGAGGGGGCCGTTGGGGGCAATGGGAGAGAAGCACGGAACCC
CAGAGAGAGGCTGAGCAGGAAGGAGGCCAGGTTTTGCACAGCGAGGAAGTGCCTCTTCTG
TACTTGGGCCGGGTGCACTGGCGCGTGAACCCCTCTCGGACACCGGGGGGCAGGCACCGC
GTATCAGAGGAGCAGTGGCCTCACACCTGGGGCCCCTTTCCACCCTATGCCTCAGGCACG
GGGTATGTGCTGTCAGCGTCTGCTGTGCAGCTCATTCTCAAGGTGGCCAGCCGGGCACCC
CTTCTCCCATTAGAGGATGTCTTTGTGGGGGTAAGTGCCCGACGAGGAGGCCTCGCCCCA
ACACAGTGTGTCAAGCTGGCTGGTGCCACCCACTACCCGCTAGACCGGTGCTGCTATGGG
AAATTCCTGCTGACGTCCCACAGGCTGGACCCCTGGAAGATGCAGGAAGCCTGGAAGCTG
GTGGGTGGCTCTGACGGGGAAAGGACTGCGCCCTTTTGCTCCTGGTTCCAGGGAGTCCTG
GGCATCCTGCGGTGTCGAGCAATAGCCTGGCTTCAGAGC

Protein sequence predicted based on nucleotide sequence

SGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLN
QRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQD
SYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEP
QREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGT
GYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYG
KFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,75

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable