POGLUT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  POGLUT1
Gene Name:  protein O-glucosyltransferase 1
Original Clone ID:
None
Keyword:
partial cds (N and C terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358346
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1095nts
Open reading frame: 1 to 1095

CAGAAGGAGTCAGGTTCAAAATGGAAAGTATTTATTGACCAAATTAACAGGTCTTTGGAG
AATTACGAACCATGTTCAAGTCAAAACTGCAGCTGCTACCATGGTGTCATAGAAGAGGAT
CTAACTCCTTTCCGAGGAGGCATCTCCAGGAGGATGATGGCAGAGGTAGTCAGACGGAAG
CTAGGGACCCACTATCAGATCACTAAGAACAGACTGTACCGGGAAAATGACTGCATGTTC
CCCTCAAGGTGTAGCGGTGTTGAGCACTTTATTTTGGAAGTGATCGGGCGTCTCCCTGAC
ATGGAGATGGTGATCAATGTACGAGATTATCCTCAGGTTCCTAAATGGATGGAGCCTGCC
ATCCCAGTCTTCTCCTTCAGTAAGACATCAGAGTACCATGATATCATGTATCCTGCTTGG
ACATTTTGGGAAGGGGGACCTGCTGTTTGGCCAATTTATCCTACAGGTCTTGGACGGTGG
GACCTCTTCAGAGAAGATCTGGTAAGGTCAGCAGCACAGTGGCCATGGAAAAAGAAAAAC
TCTACAGCATATTTCCGAGGATCAAGGACAAGTCCAGAACGAGATCCTCTCATTCTTCTG
TCTCGGAAAAACACAAAACTTGTTGATGCAGAATACACCAAAAACCAGGCCTGGAAATCT
ATGAAAGATACCTTAGGAAAGCCAGCTGCTAAGGATGTCCATCTTGTGGATCACTGCAAA
TACAAGTATCTGTTTAATTTTCGAGGCGTAGCTGCAAGTTTCCGGTTTAAACACCTCTTC
CTGTGTGGCTCACTTGTTTTCCATGTTGGTGATGAGTGGCTAGAATTCTTCTATCCACAG
CTGAAGCCATGGGTTCACTATATCCCAGTCAAAACAGATCTCTCCAATGTCCAAGAGCTG
TTACAATTTGTAAAAGCAAATGATGATGTAGCTCAAGAGATTGCTGAAAGGGGAAGCCAG
TTTATTAGGAACCATTTGCAGATGGATGACATCACCTGTTACTGGGAGAACCTCTTGAGT
GAATACTCTAAATTCCTGTCTTATAATGTAACGAGAAGGAAAGGTTATGATCAAATTATT
CCCAAAATGTTGTAG

Protein sequence predicted based on nucleotide sequence

QKESGSKWKVFIDQINRSLENYEPCSSQNCSCYHGVIEEDLTPFRGGISRRMMAEVVRRK
LGTHYQITKNRLYRENDCMFPSRCSGVEHFILEVIGRLPDMEMVINVRDYPQVPKWMEPA
IPVFSFSKTSEYHDIMYPAWTFWEGGPAVWPIYPTGLGRWDLFREDLVRSAAQWPWKKKN
STAYFRGSRTSPERDPLILLSRKNTKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLVDHCK
YKYLFNFRGVAASFRFKHLFLCGSLVFHVGDEWLEFFYPQLKPWVHYIPVKTDLSNVQEL
LQFVKANDDVAQEIAERGSQFIRNHLQMDDITCYWENLLSEYSKFLSYNVTRRKGYDQII
PKML*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,72; del@1165,15

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable