CHI3L2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  CHI3L2
Gene Name:  chitinase 3-like 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358347
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1092nts
Open reading frame: 1 to 1092

TACAAACTGGTTTGCTACTTTACCAACTGGTCCCAGGACCGGCAGGAACCAGGAAAATTC
ACCCCTGAGAATATTGACCCCTTCCTATGCTCTCATCTCATCTATTCATTCGCCAGCATC
GAAAACAACAAGGTTATCATCAAGGACAAGAGTGAAGTGATGCTCTACCAGACCATCAAC
AGTCTCAAAACCAAGAATCCCAAACTGAAAATTCTCTTGTCCATTGGAGGGTACCTGTTT
GGTTCCAAAGGGTTCCACCCTATGGTGGATTCTTCTACATCACGCTTGGAATTCATTAAC
TCCATAATCCTGTTTCTGAGGAACCATAACTTTGATGGACTGGATGTAAGCTGGATCTAC
CCAGATCAGAAAGAAAACACTCATTTCACTGTGCTGATTCATGAGTTAGCAGAAGCCTTT
CAGAAGGACTTCACAAAATCCACCAAGGAAAGGCTTCTCTTGACTGTGGGCGTATCTGCA
GGGAGGCAAATGATTGATAACAGCTATCAAGTTGAGAAACTGGCAAAAGATCTGGATTTC
ATCAACCTCCTGTCCTTTGACTTCCATGGGTCTTGGGAAAAGCCCCTTATCACTGGCCAC
AACAGCCCTCTGAGCAAGGGGTGGCAGGACAGAGGGCCAAGCTCCTACTACAATGTGGAA
TATGCTGTGGGGTACTGGATACATAAGGGAATGCCATCAGAGAAGGTGGTCATGGGCATC
CCCACATATGGGCACTCCTTCACACTGGCCTCTGCAGAAACCACCGTGGGGGCCCCTGCC
TCTGGCCCTGGAGCTGCTGGACCCATCACAGAGTCTTCAGGCTTCCTGGCCTATTATGAG
ATCTGCCAGTTCCTGAAAGGAGCCAAGATCACGCGGCTCCAGGATCAGCAGGTTCCCTAC
GCAGTCAAGGGGAACCAGTGGGTGGGCTATGATGATGTGAAGAGTATGGAGACCAAGGTT
CAGTTCTTAAAGAATTTAAACCTGGGAGGAGCCATGATCTGGTCTATTGACATGGATGAC
TTCACTGGCAAATCCTGCAACCAGGGCCCTTACCCTCTTGTCCAAGCAGTCAAGAGAAGC
CTTGGCTCCCTG

Protein sequence predicted based on nucleotide sequence

YKLVCYFTNWSQDRQEPGKFTPENIDPFLCSHLIYSFASIENNKVIIKDKSEVMLYQTIN
SLKTKNPKLKILLSIGGYLFGSKGFHPMVDSSTSRLEFINSIILFLRNHNFDGLDVSWIY
PDQKENTHFTVLIHELAEAFQKDFTKSTKERLLLTVGVSAGRQMIDNSYQVEKLAKDLDF
INLLSFDFHGSWEKPLITGHNSPLSKGWQDRGPSSYYNVEYAVGYWIHKGMPSEKVVMGI
PTYGHSFTLASAETTVGAPASGPGAAGPITESSGFLAYYEICQFLKGAKITRLQDQQVPY
AVKGNQWVGYDDVKSMETKVQFLKNLNLGGAMIWSIDMDDFTGKSCNQGPYPLVQAVKRS
LGSL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,78

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable