GXYLT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  GXYLT1
Gene Name:  glucoside xylosyltransferase 1
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358350
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1131nts
Open reading frame: 1 to 1131

CCCGCAGCGCATCCCGGCGTGTCGGACAGGTGTAAAGATTTCTCTCTGTGTTACTGGAAT
CCCTATTGGATGCTGCCCTCTGATGTTTGTGGAATGAACTGCTTTTGGGAAGCGGCTTTT
AGGTACAGTCTGAAAATACAGCCTGTTGAGAAAATGCATCTAGCTGTAGTTGCCTGTGGT
GAAAGACTGGAAGAAACTATGACCATGTTGAAGTCAGCTATCATTTTCAGCATCAAACCT
CTTCAATTCCATATTTTTGCTGAAGATCAGCTACATCATAGCTTTAAAGGCAGACTTGAC
AACTGGTCATTTCTACAAACATTTAATTATACGTTATACCCCATAACCTTTCCAAGTGAG
AATGCAGCAGAGTGGAAAAAACTCTTTAAACCATGTGCTTCGCAGAGATTGTTCTTGCCG
TTAATCCTGAAAGAAGTTAACTCACTATTGTATGTCGACACTGATATCCTTTTTTTACGA
CCAGTTGATGATATTTGGTCTTTACTAAAGAAATTTAATTCCACACAAATTGCTGCAATG
GCACCAGAACATGAGGAACCTCGAATAGGATGGTATAATCGCTTTGCTAGGCATCCATAT
TATGGAAAAACTGGAGTAAACTCTGGAGTTATGTTGATGAACATGACTCGAATGAGAAGG
AAGTATTTCAAGAATGATATGACAACTGTACGACTACAATGGGGAGATATACTTATGCCA
TTGCTTAAAAAATACAAACTAAACATCACATGGGGTGATCAAGATCTATTGAATATCGTG
TTTTTTCATAATCCAGAAAGCCTTTTTGTTTTTCCGTGTCAATGGAATTATCGACCAGAT
CATTGTATATATGGAAGCAATTGCCAAGAAGCAGAAGAAGGAGGAATCTTTATTCTTCAT
GGGAACAGAGGTGTTTACCATGACGATAAGCAACCAGCATTTAGAGCTGTTTATGAAGCA
CTGAGAAATTGTTCTTTTGAAGATGACAACATCCGTTCCTTATTAAAACCTTTAGAACTG
GAACTACAAAAAACAGTGCATACATACTGTGGAAAAATTTACAAAATATTTATCAAACAA
CTAGCAAAAAGTGTAAGAGATCGTTATGCCAGATCACCAAAGGAAAAGTAG

Protein sequence predicted based on nucleotide sequence

PAAHPGVSDRCKDFSLCYWNPYWMLPSDVCGMNCFWEAAFRYSLKIQPVEKMHLAVVACG
ERLEETMTMLKSAIIFSIKPLQFHIFAEDQLHHSFKGRLDNWSFLQTFNYTLYPITFPSE
NAAEWKKLFKPCASQRLFLPLILKEVNSLLYVDTDILFLRPVDDIWSLLKKFNSTQIAAM
APEHEEPRIGWYNRFARHPYYGKTGVNSGVMLMNMTRMRRKYFKNDMTTVRLQWGDILMP
LLKKYKLNITWGDQDLLNIVFFHNPESLFVFPCQWNYRPDHCIYGSNCQEAEEGGIFILH
GNRGVYHDDKQPAFRAVYEALRNCSFEDDNIRSLLKPLELELQKTVHTYCGKIYKIFIKQ
LAKSVRDRYARSPKEK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,192

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable