POFUT2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  POFUT2
Gene Name:  protein O-fucosyltransferase 2
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358354
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1227nts
Open reading frame: 1 to 1227

TCCGGCCAGGAGTTCTGGCCCGGACAATCGGCGGCCGATATTCTGTCGGGGGCGGCTTCC
CGCAGACGGTATCTTCTGTATGACGTCAACCCCCCGGAAGGCTTCAACCTGCGCAGGGAT
GTCTATATCCGAATCGCCTCTCTCCTGAAGACTCTGCTGAAGACGGAGGAGTGGGTGCTT
GTCCTGCCTCCATGGGGCCGCCTCTATCACTGGCAGAGTCCTGACATCCACCAGGTCCGG
ATTCCCTGGTCTGAGTTTTTTGATCTTCCAAGTCTCAATAAAAACATCCCCGTCATCGAG
TATGAGCAGTTCATCGCAGAATCTGGTGGGCCCTTTATTGACCAGGTTTACGTCCTGCAA
AGTTACGCAGAGGGGTGGAAAGAAGGGACCTGGGAAGAGAAGGTGGACGAGCGGCCGTGT
ATTGATCAGCTCCTGTACTCCCAGGACAAGCACGAGTACTACAGAGGATGGTTTTGGGGT
TATGAGGAGACCAGGGGTCTAAACGTCTCCTGTCTGTCCGTCCAGGGCTCAGCCTCCATC
GTGGCGCCCCTGCTGCTGAGAAACACATCAGCCCGGTCCGTGATGTTAGACAGAGCCGAG
AACCTACTTCACGACCACTATGGAGGGAAAGAATACTGGGATACCCGTCGCAGCATGGTG
TTTGCCAGGCACCTGCGGGAGGTGGGAGACGAGTTCAGGAGCAGACATCTCAACTCCACG
GACGACGCAGACAGGATCCCCTTCCAGGAGGACTGGATGAAGATGAAGGTCAAGCTGGGC
TCCGCGCTAGGGGGCCCCTACCTGGGAGTCCACCTGAGAAGAAAAGATTTCATCTGGGGT
CACAGACAGGATGTACCCAGTCTGGAAGGGGCCGTGAGGAAGATCCGCAGCCTCATGAAG
ACCCACCGGCTGGACAAGGTGTTTGTGGCCACAGATGCCGTCAGAAAGGAATATGAAGAG
CTAAAAAAGCTGTTACCCGAGATGGTGAGGTTTGAACCCACGTGGGAGGAGCTGGAGCTC
TACAAGGACGGAGGCGTTGCGATTATTGACCAGTGGATCTGCGCACACGCCAGGTTTTTT
ATTGGCACCTCAGTCTCAACATTTTCTTTTCGGATTCATGAGGAAAGAGAAATCCTGGGG
TTGGACCCCAAGACGACGTACAACAGGTTCTGCGGAGACCAAGAGAAGGCGTGTGAGCAA
CCCACCCACTGGAAGATCACCTACTAG

Protein sequence predicted based on nucleotide sequence

SGQEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRIASLLKTLLKTEEWVL
VLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQ
SYAEGWKEGTWEEKVDERPCIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASI
VAPLLLRNTSARSVMLDRAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNST
DDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMK
THRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFF
IGTSVSTFSFRIHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKITY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,63

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable