HPSE (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  HPSE
Gene Name:  heparanase
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358371
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1524nts
Open reading frame: 1 to 1524

CAGGACGTCGTGGACCTGGACTTCTTCACCCAGGAGCCGCTGCACCTGGTGAGCCCCTCG
TTCCTGTCCGTCACCATTGACGCCAACCTGGCCACGGACCCGCGGTTCCTCATCCTCCTG
GGTTCTCCAAAGCTTCGTACCTTGGCCAGAGGCTTGTCTCCTGCGTACCTGAGGTTTGGT
GGCACCAAGACAGACTTCCTAATTTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGA
AGTTACTGGCAATCTCAAGTCAACCAGGATATTTGCAAATATGGATCCATCCCTCCTGAT
GTGGAGGAGAAGTTACGGTTGGAATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACAC
TACCAGAAAAAGTTCAAGAACAGCACCTACTCAAGAAGCTCTGTAGATGTGCTATACACT
TTTGCAAACTGCTCAGGACTGGACTTGATCTTTGGCCTAAATGCGTTATTAAGAACAGCA
GATTTGCAGTGGAACAGTTCTAATGCTCAGTTGCTCCTGGACTACTGCTCTTCCAAGGGG
TATAACATTTCTTGGGAACTAGGCAATGAACCTAACAGTTTCCTTAAGAAGGCTGATATT
TTCATCAATGGGTCGCAGTTAGGAGAAGATTTTATTCAATTGCATAAACTTCTAAGAAAG
TCCACCTTCAAAAATGCAAAACTCTATGGTCCTGATGTTGGTCAGCCTCGAAGAAAGACG
GCTAAGATGCTGAAGAGCTTCCTGAAGGCTGGTGGAGAAGTGATTGATTCAGTTACATGG
CATCACTACTATTTGAATGGACGGACTGCTACCAGGGAAGATTTTCTAAACCCTGATGTA
TTGGACATTTTTATTTCATCTGTGCAAAAAGTTTTCCAGGTGGTTGAGAGCACCAGGCCT
GGCAAGAAGGTCTGGTTAGGAGAAACAAGCTCTGCATATGGAGGCGGAGCGCCCTTGCTA
TCCGACACCTTTGCAGCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCCGAATG
GGAATAGAAGTGGTGATGAGGCAAGTATTCTTTGGAGCAGGAAACTACCATTTAGTGGAT
GAAAACTTCGATCCTTTACCTGATTATTGGCTATCTCTTCTGTTCAAGAAATTGGTGGGC
ACCAAGGTGTTAATGGCAAGCGTGCAAGGTTCAAAGAGAAGGAAGCTTCGAGTATACCTT
CATTGCACAAACACTGACAATCCAAGGTATAAAGAAGGAGATTTAACTCTGTATGCCATA
AACCTCCATAATGTCACCAAGTACTTGCGGTTACCCTATCCTTTTTCTAACAAGCAAGTG
GATAAATACCTTCTAAGACCTTTGGGACCTCATGGATTACTTTCCAAATCTGTCCAACTC
AATGGTCTAACTCTAAAGATGGTGGATGATCAAACCTTGCCACCTTTAATGGAAAAACCT
CTCCGGCCAGGAAGTTCACTGGGCTTGCCAGCTTTCTCATATAGTTTTTTTGTGATAAGA
AATGCCAAAGTTGCTGCTTGCATC

Protein sequence predicted based on nucleotide sequence

QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFG
GTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREH
YQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKG
YNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKT
AKMLKSFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTRP
GKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYHLVD
ENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDLTLYAI
NLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTLPPLMEKP
LRPGSSLGLPAFSYSFFVIRNAKVAACI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,105

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable