EXT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  EXT1
Gene Name:  exostosin glycosyltransferase 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358381
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2133nts
Open reading frame: 1 to 2133

CGGAGAGAAGAACACAGCGGTAGGAATGGCTTGCACCACCCCAGTCCGGATCATTTCTGG
CCCCGCTTCCCGGACGCTCTGCGCCCCTTCGTTCCTTGGGATCAATTGGAAAACGAGGAT
TCCAGCGTGCACATTTCCCCCCGGCAGAAGCGAGATGCCAACTCCAGCATCTACAAAGGC
AAGAAGTGCCGCATGGAGTCCTGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAA
GTCTACGTATACCCACAGCAAAAAGGGGAGAAAATCGCCGAAAGTTACCAAAACATTCTA
GCGGCCATCGAGGGCTCCAGGTTCTACACCTCGGACCCCAGCCAGGCGTGCCTCTTTGTC
CTGAGTCTGGATACTTTAGACAGAGACCAGTTGTCACCTCAGTATGTGCACAATTTGAGA
TCCAAAGTGCAGAGTCTCCACTTGTGGAACAATGGTAGGAATCATTTAATTTTTAATTTA
TATTCCGGCACTTGGCCTGACTACACCGAGGACGTGGGGTTTGACATCGGCCAGGCGATG
CTGGCCAAAGCCAGCATCAGTACTGAAAACTTCCGACCCAACTTTGATGTTTCTATTCCC
CTCTTTTCTAAGGATCATCCCAGGACAGGAGGGGAGAGGGGGTTTTTGAAGTTCAACACC
ATCCCTCCTCTCAGGAAGTACATGCTGGTATTCAAGGGGAAGAGGTACCTGACAGGGATA
GGATCAGACACCAGGAATGCCTTATATCACGTCCATAACGGGGAGGACGTTGTGCTCCTC
ACCACCTGCAAGCATGGCAAAGACTGGCAAAAGCACAAGGATTCTCGCTGTGACAGAGAC
AACACCGAGTATGAGAAGTATGATTATCGGGAAATGCTGCACAATGCCACTTTCTGTCTG
GTTCCTCGTGGTCGCAGGCTTGGGTCCTTCAGATTCCTGGAGGCTTTGCAGGCTGCCTGC
GTCCCTGTGATGCTCAGCAATGGATGGGAGTTGCCATTCTCTGAAGTGATTAATTGGAAC
CAAGCTGCCGTCATAGGCGATGAGAGATTGTTATTACAGATTCCTTCTACAATCAGGTCT
ATTCATCAGGATAAAATCCTAGCACTTAGACAGCAGACACAATTCTTGTGGGAGGCTTAT
TTTTCTTCAGTTGAGAAGATTGTATTAACTACACTAGAGATTATTCAGGACAGAATATTC
AAGCACATATCACGTAACAGTTTAATATGGAACAAACATCCTGGAGGATTGTTCGTACTA
CCACAGTATTCATCTTATCTGGGAGATTTTCCTTACTACTATGCTAATTTAGGTTTAAAG
CCCCCCTCCAAATTCACTGCAGTCATCCATGCGGTGACCCCCCTGGTCTCTCAGTCCCAG
CCAGTGTTGAAGCTTCTCGTGGCTGCAGCCAAGTCCCAGTACTGTGCCCAGATCATAGTT
CTATGGAATTGTGACAAGCCCCTACCAGCCAAACACCGCTGGCCTGCCACTGCTGTGCCT
GTCGTCGTCATTGAAGGAGAGAGCAAGGTTATGAGCAGCCGTTTTCTGCCCTACGACAAC
ATCATCACAGACGCCGTGCTCAGCCTTGACGAGGACACGGTGCTTTCAACAACAGAGGTG
GATTTCGCCTTCACAGTGTGGCAGAGCTTCCCTGAGAGGATTGTGGGGTACCCCGCGCGC
AGCCACTTCTGGGATAACTCTAAGGAGCGGTGGGGATACACATCAAAGTGGACGAACGAC
TACTCCATGGTGTTGACAGGAGCTGCTATTTACCACAAATATTATCACTACCTATACTCC
CATTACCTGCCAGCCAGCCTGAAGAACATGGTGGACCAATTGGCCAATTGTGAGGACATT
CTCATGAACTTCCTGGTGTCTGCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAG
AAGCAGTATAAGGAGACAATGATGGGACAGACTTCTCGGGCTTCCCGTTGGGCTGACCCT
GACCACTTTGCCCAGCGACAGAGCTGCATGAATACGTTTGCCAGCTGGTTTGGCTACATG
CCGCTGATCCACTCTCAGATGAGGCTCGACCCCGTCCTCTTTAAAGACCAGGTCTCTATT
TTGAGGAAGAAATACCGAGACATTGAGCGACTT

Protein sequence predicted based on nucleotide sequence

RREEHSGRNGLHHPSPDHFWPRFPDALRPFVPWDQLENEDSSVHISPRQKRDANSSIYKG
KKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFV
LSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAM
LAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGI
GSDTRNALYHVHNGEDVVLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCL
VPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRS
IHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVL
PQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIV
LWNCDKPLPAKHRWPATAVPVVVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEV
DFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYS
HYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADP
DHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,105

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable