GALNT10 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  GALNT10
Gene Name:  UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358388
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1647nts
Open reading frame: 1 to 1647

CACAGTCGACAAAAGAAAACGTTTTTCTTGGGAGATGGGCAGAAGCTGAAGGACTGGCAT
GACAAGGAGGCCATCCGGAGGGACGCTCAGCGCGTAGGAAATGGAGAACAAGGAAGACCT
TACCCCATGACCGATGCTGAGAGAGTGGATCAGGCATACCGAGAAAATGGATTTAACATC
TACGTCAGTGATAAAATCTCCTTGAATCGCTCTCTCCCAGATATCCGGCACCCAAACTGC
AACAGCAAGCGCTACCTGGAGACACTTCCCAACACAAGCATCATCATCCCCTTCCACAAC
GAGGGCTGGTCCTCCCTCCTCCGCACCGTCCACAGTGTGCTCAATCGCTCGCCTCCAGAG
CTGGTCGCCGAGATTGTACTGGTCGACGACTTCAGTGATCGAGAGCACCTGAAGAAGCCT
CTTGAAGACTACATGGCCCTTTTCCCCAGTGTGAGGATTCTTCGAACCAAGAAACGGGAA
GGGCTGATAAGGACCCGAATGCTGGGGGCCTCAGTGGCAACTGGGGATGTCATCACATTC
TTGGATTCACACTGTGAAGCCAATGTCAACTGGCTTCCCCCCTTGCTTGACCGCATTGCT
CGGAACCGCAAGACCATTGTGTGCCCGATGATTGATGTAATTGACCATGACGACTTTCGG
TACGAGACACAGGCAGGGGATGCCATGCGGGGAGCCTTTGACTGGGAGATGTACTACAAG
CGGATCCCGATCCCTCCAGAACTGCAGAAAGCTGACCCCAGCGACCCATTTGAGTCTCCC
GTGATGGCCGGTGGACTGTTCGCCGTGGATCGGAAGTGGTTCTGGGAACTCGGCGGGTAT
GACCCAGGCTTGGAGATCTGGGGAGGGGAGCAGTATGAAATCTCCTTCAAGGTGTGGATG
TGTGGGGGCCGCATGGAGGACATCCCCTGCTCCAGGGTGGGCCATATCTACAGGAAGTAT
GTGCCCTACAAGGTCCCGGCCGGAGTCAGCCTGGCCCGGAACCTTAAGCGGGTGGCCGAA
GTGTGGATGGATGAGTACGCAGAGTACATTTACCAGCGCCGGCCTGAATACCGCCACCTC
TCCGCTGGGGATGTCGCAGTCCAGAAAAAGCTCCGCAGCTCCCTTAACTGCAAGAGTTTC
AAGTGGTTTATGACGAAGATAGCCTGGGACCTGCCCAAATTCTACCCACCCGTGGAGCCC
CCGGCTGCAGCTTGGGGGGAGATCCGAAATGTGGGCACAGGGCTGTGTGCAGACACAAAG
CACGGGGCCTTGGGCTCCCCACTAAGGCTAGAGGGCTGCGTCCGAGGCCGTGGGGAGGCT
GCCTGGAACAACATGCAGGTATTCACCTTCACCTGGAGAGAGGACATCCGGCCTGGAGAC
CCCCAGCACACCAAGAAGTTCTGCTTTGATGCCATTTCCCACACCAGCCCTGTCACGCTG
TACGACTGCCACAGCATGAAGGGCAACCAGCTGTGGAAATACCGCAAAGACAAGACCCTG
TACCACCCTGTCAGTGGCAGCTGCATGGACTGCAGTGAAAGTGACCATAGGATCTTCATG
AACACCTGCAACCCATCCTCTCTCACCCAGCAGTGGCTGTTTGAACACACCAACTCAACA
GTCTTGGAAAAATTCAATAGGAACTAG

Protein sequence predicted based on nucleotide sequence

HSRQKKTFFLGDGQKLKDWHDKEAIRRDAQRVGNGEQGRPYPMTDAERVDQAYRENGFNI
YVSDKISLNRSLPDIRHPNCNSKRYLETLPNTSIIIPFHNEGWSSLLRTVHSVLNRSPPE
LVAEIVLVDDFSDREHLKKPLEDYMALFPSVRILRTKKREGLIRTRMLGASVATGDVITF
LDSHCEANVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRYETQAGDAMRGAFDWEMYYK
RIPIPPELQKADPSDPFESPVMAGGLFAVDRKWFWELGGYDPGLEIWGGEQYEISFKVWM
CGGRMEDIPCSRVGHIYRKYVPYKVPAGVSLARNLKRVAEVWMDEYAEYIYQRRPEYRHL
SAGDVAVQKKLRSSLNCKSFKWFMTKIAWDLPKFYPPVEPPAAAWGEIRNVGTGLCADTK
HGALGSPLRLEGCVRGRGEAAWNNMQVFTFTWREDIRPGDPQHTKKFCFDAISHTSPVTL
YDCHSMKGNQLWKYRKDKTLYHPVSGSCMDCSESDHRIFMNTCNPSSLTQQWLFEHTNST
VLEKFNRN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,165

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable