GCNT2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  GCNT2
Gene Name:  glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358391
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1128nts
Open reading frame: 1 to 1128

AATTTTGGGGGAGATCCAAGCTTCCAAAGGCTAAATATCTCAGACCCTTTGAGGCTGACT
CAAGTTTGCACATCTTTTATCAATGGAAAAACACGTTTCCTGTGGAAAAACAAACTAATG
ATCCATGAGAAGTCTTCTTGCAAGGAATACTTGACCCAGAGCCACTACATCACAGCCCCT
TTATCTAAGGAAGAAGCTGACTTTCCCTTGGCATATATAATGGTCATCCATCATCACTTT
GACACCTTTGCAAGGCTCTTCAGGGCTATTTACATGCCCCAAAATATCTACTGTGTTCAT
GTGGATGAAAAAGCAACAACTGAATTTAAAGATGCGGTAGAGCAACTATTAAGCTGCTTC
CCAAACGCTTTTCTGGCTTCCAAGATGGAACCCGTTGTCTATGGAGGGATCTCCAGGCTC
CAGGCTGACCTGAACTGCATCAGAGATCTTTCTGCCTTCGAGGTCTCATGGAAGTACGTT
ATCAACACCTGTGGGCAAGACTTCCCCCTGAAAACCAACAAGGAAATAGTTCAGTATCTG
AAAGGATTTAAAGGTAAAAATATCACCCCAGGGGTGCTGCCCCCAGCTCATGCAATTGGA
CGGACTAAATATGTCCACCAAGAGCACCTGGGCAAAGAGCTTTCCTATGTGATAAGAACA
ACAGCGTTGAAACCGCCTCCCCCCCATAATCTCACAATTTACTTTGGCTCTGCCTATGTG
GCTCTATCAAGAGAGTTTGCCAACTTTGTTCTGCATGACCCACGGGCTGTTGATTTGCTC
CAGTGGTCCAAGGACACTTTCAGTCCTGATGAGCATTTCTGGGTGACACTCAATAGGATT
CCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAACCTCAGAGCTATAAAG
TGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTATGTACATGGTATTTGT
ATCTATGGAAACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGCCTGTTTGCTAACAAG
TTTGAGCTTAATACCTACCCCCTTACTGTGGAATGCCTAGAACTGAGGCATCGCGAAAGA
ACCCTCAATCAGAGTGAAACTGCGATACAACCCAGCTGGTATTTTTAG

Protein sequence predicted based on nucleotide sequence

NFGGDPSFQRLNISDPLRLTQVCTSFINGKTRFLWKNKLMIHEKSSCKEYLTQSHYITAP
LSKEEADFPLAYIMVIHHHFDTFARLFRAIYMPQNIYCVHVDEKATTEFKDAVEQLLSCF
PNAFLASKMEPVVYGGISRLQADLNCIRDLSAFEVSWKYVINTCGQDFPLKTNKEIVQYL
KGFKGKNITPGVLPPAHAIGRTKYVHQEHLGKELSYVIRTTALKPPPPHNLTIYFGSAYV
ALSREFANFVLHDPRAVDLLQWSKDTFSPDEHFWVTLNRIPGVPGSMPNASWTGNLRAIK
WSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRER
TLNQSETAIQPSWYF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,75

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable