GLT25D2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  COLGALT2
Gene Name:  collagen beta(1-O)galactosyltransferase 2
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358404
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1800nts
Open reading frame: 1 to 1800

CGCTTCGTCGCCGAGCGGGACTCGGAGGACGACGGAGAGGAGCCGGTGGTTTTCCCGGAG
TCGCCCCTGCAGAGCCCCACGGTGCTCGTGGCGGTCCTCGCCCGCAACGCGGCGCACACG
CTGCCGCACTTCCTCGGCTGCCTGGAGCGGCTGGACTACCCCAAGAGCAGGATGGCCATC
TGGGCAGCCACTGATCACAATGTGGATAATACAACAGAAATATTCAGGGAGTGGTTGAAA
AATGTACAGAGACTCTATCACTATGTGGAGTGGAGGCCTATGGATGAACCAGAGTCTTAC
CCTGATGAAATTGGACCAAAGCACTGGCCAACCTCCCGGTTTGCCCATGTGATGAAACTA
CGACAGGCAGCCCTTCGAACTGCGAGGGAAAAATGGTCAGACTACATTCTGTTCATAGAT
GTTGACAATTTCCTGACTAATCCACAGACCCTCAATCTACTGATTGCAGAAAACAAAACT
ATTGTGGCCCCCATGCTGGAGTCTCGGGGCCTGTATTCTAATTTCTGGTGCGGAATCACC
CCTAAGGGCTTCTATAAGAGGACCCCAGACTACGTTCAGATTCGAGAATGGAAGAGGACA
GGCTGCTTCCCCGTCCCCATGGTCCACTCCACCTTCCTAATTGACCTCAGGAAGGAGGCC
TCGGACAAGCTGACTTTCTACCCCCCACACCAGGACTACACCTGGACCTTTGATGACATC
ATTGTCTTTGCCTTCTCCAGCAGGCAAGCAGGCATCCAGATGTACCTCTGCAACAGAGAG
CACTATGGCTACCTGCCCATCCCCCTGAAGCCCCATCAGACACTGCAGGAAGACATCGAG
AACCTCATCCATGTGCAGATTGAAGCAATGATTGACCGTCCTCCAATGGAACCCTCCCAG
TATGTCTCAGTTGTCCCTAAATATCCAGACAAGATGGGATTTGATGAGATTTTCATGATA
AACCTCAAACGCAGAAAGGACAGGCGGGACCGGATGCTGCGCACACTGTATGAACAGGAG
ATTGAGGTCAAGATTGTCGAGGCTGTGGATGGAAAGGCACTCAACACAAGCCAGCTGAAG
GCACTGAATATTGAAATGCTGCCTGGCTATCGAGATCCCTATTCCTCCAGGCCTCTAACA
AGGGGTGAAATCGGCTGCTTTCTCAGCCACTACTCAGTCTGGAAAGAGGTAATTGATCGA
GAGCTAGAGAAGACTCTTGTAATTGAAGACGATGTGCGTTTTGAGCATCAGTTTAAGAAG
AAGCTGATGAAGCTGATGGATAACATTGACCAGGCTCAGCTGGACTGGGAACTGATTTAT
ATTGGTAGGAAGAGGATGCAAGTAAAGGAGCCAGAGAAAGCAGTGCCCAATGTGGCAAAC
CTGGTCGAAGCCGACTATTCCTACTGGACCCTGGGCTACGTCATCTCTCTGGAAGGAGCA
CAGAAGCTGGTTGGAGCCAATCCTTTTGGGAAGATGCTGCCAGTGGATGAGTTTCTGCCA
GTCATGTACAACAAGCATCCCGTAGCCGAGTACAAGGAGTATTATGAATCCAGGGACCTG
AAAGCCTTCTCTGCAGAACCCTTGCTCATCTACCCTACGCACTACACAGGCCAGCCGGGG
TACCTGAGTGACACGGAGACCTCCACCATCTGGGACAATGAGACAGTGGCCACCGACTGG
GATAGGACACATGCCTGGAAGTCCCGGAAGCAAAGCCGCATCTACAGCAATGCCAAGAAC
ACAGAGGCCCTGCCACCGCCAACCTCCCTGGACACTGTGCCTTCAAGGGATGAGCTATAG

Protein sequence predicted based on nucleotide sequence

RFVAERDSEDDGEEPVVFPESPLQSPTVLVAVLARNAAHTLPHFLGCLERLDYPKSRMAI
WAATDHNVDNTTEIFREWLKNVQRLYHYVEWRPMDEPESYPDEIGPKHWPTSRFAHVMKL
RQAALRTAREKWSDYILFIDVDNFLTNPQTLNLLIAENKTIVAPMLESRGLYSNFWCGIT
PKGFYKRTPDYVQIREWKRTGCFPVPMVHSTFLIDLRKEASDKLTFYPPHQDYTWTFDDI
IVFAFSSRQAGIQMYLCNREHYGYLPIPLKPHQTLQEDIENLIHVQIEAMIDRPPMEPSQ
YVSVVPKYPDKMGFDEIFMINLKRRKDRRDRMLRTLYEQEIEVKIVEAVDGKALNTSQLK
ALNIEMLPGYRDPYSSRPLTRGEIGCFLSHYSVWKEVIDRELEKTLVIEDDVRFEHQFKK
KLMKLMDNIDQAQLDWELIYIGRKRMQVKEPEKAVPNVANLVEADYSYWTLGYVISLEGA
QKLVGANPFGKMLPVDEFLPVMYNKHPVAEYKEYYESRDLKAFSAEPLLIYPTHYTGQPG
YLSDTETSTIWDNETVATDWDRTHAWKSRKQSRIYSNAKNTEALPPPTSLDTVPSRDEL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,81

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable