LARGE (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  LARGE
Gene Name:  like-glycosyltransferase
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358422
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2001nts
Open reading frame: 1 to 2001

CCATCCCATCGCCGAGGCAACCACTCCAAGACCTACTCCATGGAGGAGGGCACTGGAGAC
AGCGAGAACCTTCGGGCTGGCATCGTGGCAGGCAACAGCTCCGAGTGTGGGCAGCAGCCG
GTCGTGGAGAAATGCGAGACAATCCACGTTGCTATTGTCTGCGCCGGATACAATGCCAGC
CGGGATGTCGTCACCCTGGTCAAATCCGTCCTGTTCCATAGACGGAACCCTCTGCACTTC
CACCTTATTGCTGACTCCATTGCGGAGCAGATCCTGGCCACGCTCTTCCAGACCTGGATG
GTGCCCGCTGTGCGTGTGGACTTCTACAATGCAGACGAGCTCAAGTCTGAAGTTTCCTGG
ATCCCCAATAAACATTACTCTGGGATTTATGGTCTGATGAAGCTTGTCCTGACCAAGACT
CTTCCTGCCAACCTGGAGAGAGTCATCGTCCTTGACACGGATATCACCTTTGCCACTGAC
ATTGCAGAGCTGTGGGCTGTGTTCCACAAGTTCAAAGGTCAGCAAGTCCTGGGCTTGGTG
GAGAACCAGAGTGACTGGTACCTTGGAAACCTGTGGAAAAATCACCGCCCATGGCCAGCC
CTTGGAAGAGGCTACAACACAGGGGTGATCCTGTTACTTCTGGATAAGCTGCGGAAGATG
AAATGGGAGCAGATGTGGAGGCTGACCGCAGAGAGGGAGCTCATGGGCATGCTCTCTACA
TCCTTAGCTGACCAGGATATTTTCAATGCCGTCATCAAACAAAACCCCTTCCTTGTGTAC
CAGCTCCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCCGAGCAGTGCTAC
AGAGACGTGTCTGATCTAAAGGTCATTCACTGGAACTCCCCCAAGAAGCTCCGGGTGAAG
AACAAGCATGTGGAGTTTTTTCGCAACCTCTACCTGACCTTCCTGGAGTATGACGGCAAT
CTTCTGAGGCGGGAACTGTTTGGCTGCCCCAGTGAGGCTGATGTCAACAGTGAAAACCTC
CAGAAGCAGCTGTCTGAGCTGGACGAGGACGACCTGTGCTATGAGTTCCGGCGAGAGCGC
TTCACTGTCCACCGCACCCACCTGTACTTCCTGCACTACGAGTATGAGCCTGCAGCAGAC
AGCACGGACGTCACCCTGGTCGCTCAGCTGTCCATGGACAGGCTCCAGATGCTGGAGGCC
ATCTGCAAGCACTGGGAGGGGCCCATCAGCCTGGCCCTCTACCTGTCAGACGCCGAGGCC
CAGCAGTTCCTCCGCTACGCACAGGGCTCTGAGGTGCTTATGAGCCGCCACAACGTGGGC
TACCACATCGTGTACAAGGAGGGCCAGTTCTACCCCGTGAACCTGCTGCGCAACGTGGCC
ATGAAGCACATCAGCACTCCCTACATGTTCCTGTCTGACATTGACTTCCTGCCCATGTAT
GGGCTCTATGAGTACCTCAGGAAGTCTGTCATCCAGCTCGATCTTGCCAACACCAAGAAA
GCAATGATTGTCCCCGCGTTCGAGACACTGCGCTACCGGCTGTCCTTCCCCAAGTCAAAA
GCGGAGTTGCTGTCAATGCTGGACATGGGGACCCTCTTCACATTCAGGTACCACGTCTGG
ACGAAAGGCCACGCACCCACAAACTTCGCCAAGTGGCGGACCGCCACCACGCCTTACCGG
GTTGAGTGGGAGGCCGATTTTGAGCCGTATGTTGTTGTGAGACGTGACTGCCCGGAGTAC
GACCGGAGGTTTGTAGGCTTTGGCTGGAACAAAGTGGCTCATATCATGGAGCTGGATGTG
CAGGAGTATGAGTTCATTGTGCTGCCCAACGCCTACATGATCCACATGCCTCATGCCCCC
AGCTTCGACATTACCAAGTTCCGTTCCAACAAGCAATACCGCATCTGTCTCAAAACCCTC
AAGGAAGAGTTTCAGCAGGACATGTCCCGCCGCTACGGCTTTGCTGCCCTGAAATATCTC
ACAGCCGAGAACAACAGCTAG

Protein sequence predicted based on nucleotide sequence

PSHRRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHVAIVCAGYNAS
RDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSW
IPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLV
ENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLST
SLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVK
NKHVEFFRNLYLTFLEYDGNLLRRELFGCPSEADVNSENLQKQLSELDEDDLCYEFRRER
FTVHRTHLYFLHYEYEPAADSTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEA
QQFLRYAQGSEVLMSRHNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMY
GLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVW
TKGHAPTNFAKWRTATTPYRVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDV
QEYEFIVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYL
TAENNS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,270

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable