ST8SIA4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ST8SIA4
Gene Name:  ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358433
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 921nts
Open reading frame: 1 to 921

AAAATCATTCGAAAGGCTGGCTCTTCAATCTTCCAGCACAATGTAGAAGGTTGGAAAATC
AATTCCTCTTTGGTCCTAGAGATAAGGAAGAACATACTTCGTTTCTTAGATGCAGAACGA
GATGTGTCAGTGGTCAAGAGCAGTTTTAAGCCTGGTGATGTCATACACTATGTGCTTGAC
AGGCGCCGGACACTAAACATTTCTCATGATCTACATAGCCTCCTACCTGAAGTTTCACCA
ATGAAGAATCGCAGGTTTAAGACCTGTGCAGTTGTTGGAAATTCTGGCATTCTGTTAGAC
AGTGAATGTGGAAAGGAGATTGACAGTCACAATTTTGTAATAAGGTGTAATCTAGCTCCT
GTGGTGGAGTTTGCTGCAGATGTGGGAACTAAATCAGATTTTATTACCATGAATCCATCA
GTTGTACAAAGAGCATTTGGAGGCTTTCGAAATGAGAGTGACAGAGAAAAATTTGTGCAT
AGACTTTCCATGCTGAATGACAGTGTCCTTTGGATTCCTGCTTTCATGGTCAAAGGAGGA
GAGAAGCACGTGGAGTGGGTTAATGCATTAATCCTTAAGAATAAACTGAAAGTGCGAACT
GCCTATCCGTCATTGAGACTTATTCATGCTGTCAGAGGTTACTGGCTGACCAACAAAGTT
CCTATCAAAAGACCCAGCACAGGTCTTCTCATGTATACACTTGCCACAAGATTCTGTGAT
GAAATTCACCTGTATGGATTCTGGCCCTTCCCTAAGGATTTAAATGGAAAAGCGGTCAAA
TATCATTATTATGATGACTTAAAATATAGGTACTTTTCCAATGCAAGCCCTCACAGAATG
CCATTAGAATTCAAAACATTAAATGTGCTACATAATAGAGGAGCTCTAAAACTGACAACA
GGAAAGTGTGTAAAGCAATAG

Protein sequence predicted based on nucleotide sequence

KIIRKAGSSIFQHNVEGWKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLD
RRRTLNISHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSECGKEIDSHNFVIRCNLAP
VVEFAADVGTKSDFITMNPSVVQRAFGGFRNESDREKFVHRLSMLNDSVLWIPAFMVKGG
EKHVEWVNALILKNKLKVRTAYPSLRLIHAVRGYWLTNKVPIKRPSTGLLMYTLATRFCD
EIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMPLEFKTLNVLHNRGALKLTT
GKCVKQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable