spoT (H. pylori )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  spoT
Gene Name:  guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
Original Clone ID:
6163a2BCt1ba1
Keyword:
full-length cds
Species:
Helicobacter pylori
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HpCD00369512
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2352nts
Open reading frame: 1 to 2352

AACGAAATTGACAAATCCGTTGATATCGGATTCTTACGGATTCTGGATGTTATTAAAAAA
GTTAAAACCCCAAAGGGTGGTATTGAGGTTTTAAGGACTTTAATTGATTTCACGCCCAAA
ATTGAAAACGCCCTAAATTTAGCGACCAAAAGCCATAAGGGGCAATACAGAAAGAGCGGT
GAGCCTTATATTGTCCATCCTATTTGCGTGGCGAGCGTGGTGGCGTTTTGTGGGGGCGAT
GAGGCGATGGTGTGCGCCGCGCTTTTGCATGATGTGGTAGAAGACACGCCTTGTGAGATT
GAAACGATTGAGCGAGAATTTGGGCAAGATGTGGCTAATTTAGTGGATGCGCTCACCAAA
ATCACTGAAATCAGGAAAGAAGAGTTAGGCGTGAGTTCTCAAGATCCCAGAATGGTGGTT
TCAGCCCTCACTTTTAGAAAGATCCTTATTAGCGCGATACAAGATCCAAGAGCCTTAGTG
GTAAAGATTAGCGACAGGTTGCACAACATGCTCACCTTAGACGCCTTGCCTCATGACAAG
CAGGTGCGTATTTCTAAAGAAACTCTAGCGGTGTATGCCCCCATAGCGAGTCGATTGGGC
ATGTCTTCAATCAAAAACGAATTAGAAGACAAGAGCTTTTATTATATTTATCCAGAAGAG
TATAAAAATATTAAGGAGTATTTGCACAAAAACAAACAGTCTTTACTCTTAAAACTCAAC
GCTTTTGCGAGCAAGTTAGAAAAAAAGCTTTTTGACAGCGGGTTTAGCCATTCGGATTTT
AAACTCGTTACAAGGGTGAAACGCCCTTATTCTATTTATCTTAAGATGCAACGAAAAGGG
GCGGTTAATATTGATGAAATTTTGGACTTGTTAGCCATTAGGATTTTATTGAAAAACCCG
ATTGATTGCTACAAGGTTTTAGGGATTATTCATTTGAATTTCAAACCCATTGTTTCTCGT
TTTAAAGATTACATCGCTTTGCCCAAAGAAAATGGCTATAAGACAATACACACGACGATT
TTTGATGAATCTTCTGTTTATGAAGTGCAGATCCGCACTTTTGATATGCACATGGGGGCG
GAGTATGGTAATTCAGCCCATTGGAAGTATAAAGCCGGGGGCGTGGATCATGAAGAACAT
CATGAGGGCATGCGGTGGTTGCAAAATTTTAAATACCATGACAGCGATTTGAAAAACGAC
CCTAAGGAATTTTACGAACTCGCTAAGAACGATTTGTATCGTGAAGATATTGTCGTTTTT
TCGCCCCATGGGGACACTTACACTTTACCGGTGGGCGCGATCGCTTTAGATTTCGCTTAC
ATGGTGCATAGTGATTTGGGCGATAAAGCCACGGACGCTTATATCAATAGTAAAAAAGCC
TTACTCAATCAAGAATTAAGGAGTGGGGATGTGGTTAAAATCATTAAAGGCGATAAAGTA
ATACCTCGTTTCATTTGGATGGATCAGCTTAAAACTTCTAAGGCTAAAAACCATTTGCGC
ATCCAAAGAAGAAACCGCTTGAAAGAAATTGACACTAAGAGCATGATCAATATCTTAGCG
ACTTTTTTTGGGCGCTCTGTTTTTGAAGATGCGGATTTGAAAGATTATAAAAACTTTGAA
GAAAAATTAACAGATTGTGGGGTGGAGACCACCTTAACAGAAGCGATGAAAAGCTTTGAA
AATTTGGCCAAACTCACTGAAGAAATTGAAAATAAGGTGTTTTCTTTAAAAGAAGACGCG
ATTTTAGAATACCAGGAGATGAGTTTATGGACTCGAGGTTTGAGATATTTGGGTTTTAAA
ACCAATGTTTTGAATTTTTTAACCCCCAACCGGCAATGGCAGTGTAAGGAATTGGAGCAT
TTTAGCGTTTGTTCAAGTAACGCTTTAGAAATCAAACAGGTGTTATTGAATGATTGTTGT
TACCCTAAATATGGCGATGAAATCATTGCGATTGTAACGGATTTGAAAGATCCAAAGGCG
ATTGCACACCATAAATTTTGCAAAAAAGCGATAGCGGAAGTGGATGCTAAAGTGCCTATG
GTTTATATAGAATGGCACAAGCGGGATCGAACGATTTATAAAATGATGTTTTATTTAGGA
GAGAAAAAGTCGGTTTTAGCGAGTTTACTGACTTTTTTAAACAGAAATGAATGCAATATT
GTGGGCGTGTCTTACTTAGGCTATAAGGACAAATATTCTAGCCATTGCGAAGTGAGTTTT
GAAATAGCCACGGATAAGGCGGATTGGATGAGGGCTTTAATCAATCGCAAATATCAGGAT
AGGATTGTAGAATTATCCAGTCTGGATGACGCTTATGAGCTACAAGAAGGGCATCATCAC
CATCACCACTGA

Protein sequence predicted based on nucleotide sequence

NEIDKSVDIGFLRILDVIKKVKTPKGGIEVLRTLIDFTPKIENALNLATKSHKGQYRKSG
EPYIVHPICVASVVAFCGGDEAMVCAALLHDVVEDTPCEIETIEREFGQDVANLVDALTK
ITEIRKEELGVSSQDPRMVVSALTFRKILISAIQDPRALVVKISDRLHNMLTLDALPHDK
QVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKLN
AFASKLEKKLFDSGFSHSDFKLVTRVKRPYSIYLKMQRKGAVNIDEILDLLAIRILLKNP
IDCYKVLGIIHLNFKPIVSRFKDYIALPKENGYKTIHTTIFDESSVYEVQIRTFDMHMGA
EYGNSAHWKYKAGGVDHEEHHEGMRWLQNFKYHDSDLKNDPKEFYELAKNDLYREDIVVF
SPHGDTYTLPVGAIALDFAYMVHSDLGDKATDAYINSKKALLNQELRSGDVVKIIKGDKV
IPRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILATFFGRSVFEDADLKDYKNFE
EKLTDCGVETTLTEAMKSFENLAKLTEEIENKVFSLKEDAILEYQEMSLWTRGLRYLGFK
TNVLNFLTPNRQWQCKELEHFSVCSSNALEIKQVLLNDCCYPKYGDEIIAIVTDLKDPKA
IAHHKFCKKAIAEVDAKVPMVYIEWHKRDRTIYKMMFYLGEKKSVLASLLTFLNRNECNI
VGVSYLGYKDKYSSHCEVSFEIATDKADWMRALINRKYQDRIVELSSLDDAYELQEGHHH
HHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6163a
Recommended expression in:
Not Applicable