Gene:
Gene Symbol:
VPA1593
Gene Name:
cyclodextrin glucanotransferase
Original Clone ID:
11097c2BCt5p1
Keyword:
full-length cds
Species:
Vibrio parahaemolyticus RIMD 2210633
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
VpCD00369560
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2274nts
Open reading frame: 1 to 2274
AATAAAATAAAAATACTTCCTTTAGCGATCGCTCTTGCTCTCTCTGGCTGTGGAAGCGAC
GAAACCACACCAATCGATACAGGCAGCCACGTGGCATCCCCTGATTGGCAAGATCAAATC
ATTTACTTCCTGATGATCGACCGTTTTAACGACGGCGACAGCGCTCTCAATGATCAAGGG
CAAAACGAATTCGATCCGACTTCGGATAAAAAATTCAGCGGCGGCGACCTCGTTGGCGTG
AGTGATAAGTTAAGCTACATCGAAGATCTCGGTGCCACATCGATCTGGATTACTCCGCCA
GTTGCTAACCAATGGTGGGATGCAGAGCAAAACTACGGCGGCTACCACGGGTACTGGGCG
CGCGATTTCCAAAAAGTGGATGAACACTTTGGTGACATGGAAAGCTACCAAGCCTTAGCA
CGCGAAGTGCATGCGCGTAACATGTTCTTAATTCAGGACATCGTGACCAACCATGTAGGC
AACTTCTTTACCTACGATGATCCTTACAGTTATGATCCAAGCCTACCTTGCCAAGGCTTC
CGTTTGATTAAAAATGCACTACCCGCCGGGCAAGAACTGCCATACCCGCTCAACATGAAC
GAGTGTAAAACAGACGGCACAGGTAGTTACCACTGGACGCCAACCATCACCGACCACAAC
GATCCGGTACAAGAAAAAACCTGGCAGCTCTCTGATTTGGATGACCTTAATACGTCCGAT
CCTGAAGTACGCGCTTATCTAAAAGAGAGCTACCGAAAATGGATTCGCGAAGTCGGCGTC
GACGGATTCCGTATCGATACGGTGAAGTTTGTTGAACACGACTTCTGGAGCGACTTCCTA
CATGCCGACGATGGTGTGATGACGCAAGCGGTTGATACCGGTCGTGACAACTTCCTCACT
TTTGGTGAAGTATTCGAAACATCTACGCCTTACCACACCGAAGGCGAAGAGAAGATGCTG
ACTTACATCGGTGAAAGTGGTTCGCCGAAACAGCTAACCTCGGTACTGAACTTCCCATTG
CAAGCGACCATGACGCGCGTATTTGCATCAGGACAACCGACCGACTATTTACGTTTCCGT
CTTGAGAAAATGATGGAGATGTTCCCAAATCCATACATCATGCCGAACTTCATTGATAAT
CACGACATGCCGCGCTTTCTGTCGCAAGGTAGCGTGAATGACATGAAACAAGCACTGATC
ACCATGATGAGCGTACCGGGTATTCCTGTGATTTACCAAGGTACAGAGCAAGCGATGTCA
GCGGCGCGTGATGCGATGTTCGCCGGAGGGTACCGTGAAGATGGCAACTACGTAGACTCA
TTCGACCAAAACAGTGAGATGTACTTGTTTATTCAAGAACTTGCCAAGCTACGCACCGAA
AACAAAGTCATGACACGTGGCGAAATAAAAGTGATTGGCAGCGACAAAGCGGGCGCGGGC
GTGTTCGCCTTTACCCGAACGTTGGATAACGACGAAGTCTTGGTGGTCATGAACACATCC
AACTCGCCGATGTTGATGAACCAACTCGATGTAGAGCAAGCGGGCGGTACCGTCTTCAAA
CAGCAAATCATGTCGAACTGGGCAGACGCTCCAGAACAGTTAGTCGCAGATGAAAATGGT
CACGTAACCCTTGAGTTGCCAGCCAAGTCAGCAGTGATTTACTCAAAAACAAGCAGCAAC
CAATCTGTAAATACCCCAGACCTCAACATCCAACTGGCGCAGGATTGGGAAGGAAAAACC
ATCACTGGTGATATTGTTATCGCGGGTAGTGCGAACGCCAACGAGAAGTTGAACCTCGTC
GTCGATGGTAACTTGGAAACAGCGCAAACCATTACTGTAGGAGCTGATGGTCAGTTTAGT
GTCACGCTGTCGACTCGTCATTTTGCGATTGGCGAGCAACAGCACCGCTTCGCGATTTAT
TCCACCGAGAAAAAAGCAGGCATCGAGGACGTGAATTTTGTCTCGAACCTAAGCTGGTCA
AACACGCCGGATGACACCATTGATGATGCAGGTGATGCACAAGACGGCGTGGGCGGCCCA
AACGGCAACTACTCATTACCAACCGATCCAACGTTCGACAAAGACAACAGCCAACTCGCG
ATCAACAAAGCAGAAGTGTTTACCGTTGGCAGTAACGTACGTTTGACCTTCACTATGGAC
AAAATCACCGACACTTGGTTGCCACCAAACGGCTTCGACCACGTGGGTTTCACCATCTTT
ATTGATTTACCGGAAGAAGCCGCAACCGAAGGGCATCATCACCATCACCACTGA
Protein sequence predicted based on nucleotide sequence
NKIKILPLAIALALSGCGSDETTPIDTGSHVASPDWQDQIIYFLMIDRFNDGDSALNDQG QNEFDPTSDKKFSGGDLVGVSDKLSYIEDLGATSIWITPPVANQWWDAEQNYGGYHGYWA RDFQKVDEHFGDMESYQALAREVHARNMFLIQDIVTNHVGNFFTYDDPYSYDPSLPCQGF RLIKNALPAGQELPYPLNMNECKTDGTGSYHWTPTITDHNDPVQEKTWQLSDLDDLNTSD PEVRAYLKESYRKWIREVGVDGFRIDTVKFVEHDFWSDFLHADDGVMTQAVDTGRDNFLT FGEVFETSTPYHTEGEEKMLTYIGESGSPKQLTSVLNFPLQATMTRVFASGQPTDYLRFR LEKMMEMFPNPYIMPNFIDNHDMPRFLSQGSVNDMKQALITMMSVPGIPVIYQGTEQAMS AARDAMFAGGYREDGNYVDSFDQNSEMYLFIQELAKLRTENKVMTRGEIKVIGSDKAGAG VFAFTRTLDNDEVLVVMNTSNSPMLMNQLDVEQAGGTVFKQQIMSNWADAPEQLVADENG HVTLELPAKSAVIYSKTSSNQSVNTPDLNIQLAQDWEGKTITGDIVIAGSANANEKLNLV VDGNLETAQTITVGADGQFSVTLSTRHFAIGEQQHRFAIYSTEKKAGIEDVNFVSNLSWS NTPDDTIDDAGDAQDGVGGPNGNYSLPTDPTFDKDNSQLAINKAEVFTVGSNVRLTFTMD KITDTWLPPNGFDHVGFTIFIDLPEEAATEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11097c
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
