Gene:
Gene Symbol:
CPR_1843
Gene Name:
FucA
Original Clone ID:
11095g6BCt9p1
Keyword:
full-length cds
Species:
Clostridium perfringens SM101
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
CpCD00369573
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2211nts
Open reading frame: 1 to 2211
GGACAAGGATCTATATTAACTAAGGCAAATACTATAAATAATATGGAATATGTAACAGAA
GTATTTGGAGCAATTCCAACAGAAGAACAGGTTAGCTATCAAAAAGAGGAACTTACAGCA
TTTATACACTTTGGTGTGAATACCTTTACGGGAAGGGAGTGGGGAGATGGAACAGAAAAT
CCAGAAATTTTTAACCCTACTAATTTAGATGCTGATCAATGGGTAAAAACTTTGGCGGAG
GCAGGCTTTGGAAGGGTTATATTAACTGCAAAACATCATGATGGATTTTGTTTGTGGGAT
AGTGCATATACAAAGCATGATGTAGCTAGTAGTCCTTGGAAAAATGGAAAGGGAGATGTG
GTTAAAGAGGTTTTAGAAGCTTGTGCTAAATATAATATAAAATTCGGAGTTTATTTGTCA
CCATGGGACCAAAATTCAGAACATTATGGAGACGGCAATGGTGGTGATTATAATGAGTTT
TACATGAATCAGTTAAGGGAATTATTAACTAACTATGGACCTATAGCTGAAGTTTGGATG
GATGGAGCAAAGGGATCGAATGTTAAGCAAGAATATAACTTTGAAGAATGGTTTGCTCTT
ATAAAAAAATTACAACCAGAATGTTTAATATTTAGTCCACAGGGGCCAGATATTAGATGG
ATTGGAAATGAAAAAGGATATGCAGGAGAACCTTGCTGGTCAACTATAGATATTGAAAAA
ATGAAAGAAAGGGAAAATCCAACATATTTGAATAATGGAGAAGAAGGTGGACCAAATTGG
ATAGTTGGAGAATCAGATGTATCCATAAGACCAGGTTGGTTTTACCATGAATCTCAAGAT
AATGAAGTTAAATCCTTAGAAAAAATGATGGATATTTATTTTAAATCCATAGGGAGAAAT
TCAGTTTTATTATTAAATGTTCCCCCTAATAAGGAAGGAAAGTTACATGAAAATGATGTT
AATAGATTAAAGAAATTTGGTGAAACAATTAAAGAGTTATTTAATGATGATTTAGCTTTA
AATAAAGAGGTAATAGTAGATGGTTTTGCTAATAATGATGAGACATATGGTGCAAATAAA
ATTGTAGATGGAGATTATGATACCTATTGGGCACCAGATAATAGTAGTAAAACAGGTACT
ATTGAAATAGATTTAGGTGGAAGTAAAGAATTTGATGTTATTTCTTTGCAAGAGTATATA
CCATTAGGTCAAAGAGTATCCAGTTTTAATGTAGAAGTATTGCAAGGAGAAAATTGGAAT
AAGGTTTATGAAGGAAAAACAATAGGATATAAAAGACTTGTTAGAATAGCTCCAACTAAA
GGAGAAAAAATAAGAATTAATATAACGGGTTCATTAGAAGTACCACTTATAAATAACGTT
GGAGTTTATAAACAACCTATTAGTATAGAACTTCCATCAGGGCCACCAGCTGGGTTGAAA
GTATTAAATGATGATAATAAGGGAAATGAATTAGAACAATTTAATTTTAGTGATGGATGG
ATATATGAGACTATCCATGGAGAAGATGATTTAGGTGGAGATGCCCATTATACAAGTAAA
ATTAATGCTACAGTTAATATTAAATTCAAGGGAACTAAGTTTTTCTTATCAGGAACAAAG
GATTCAGGACATGGAATAATGGAAATTTCAATAGATGGTGAAAATCCAGTAGAGGTTGAC
TTATATTCTCCTAATAGAAAATCTAAAGAGATAGTTTTTGAAAGTGAAGATTTAAGTGAT
GGAGAGCATGAAGTTACGGTTAAATGTACTGGAAGAAAGAATTCAAATTCTAGAGGGATA
GTGGCTCATATAGATGGAGCTTATGTATTAGACAATGGTGGAAAAGGTATGGTTGAATTT
GAAAAGGTAGGATACAAAGTAAGTGAAAATATAGGAACTGCTACTTTTAAGGTTATAAGA
AAAGGAGGAAGTAATGGTAAGCTTGAAGTTAACTATGATACTTTAGCTGGCACTGCTTTA
AATGGAGTTGATTATCAAACATGGTCTGGTACTTTAGCATTTAATGAAGGAGAAACAGAA
AAAACTTTTGATATAACAATAATTGATGATAAGGAAAAAGAAGAGCCTAAGGAATTCTAT
TTAAAATTAAGTGATCCAATAGGTGGAATATTAGGATTTAATTCAAGAGCTACAGTTATT
ATTAATGATGATGAGCAAATTAAAGAAGGGCATCATCACCATCACCACTGA
Protein sequence predicted based on nucleotide sequence
GQGSILTKANTINNMEYVTEVFGAIPTEEQVSYQKEELTAFIHFGVNTFTGREWGDGTEN PEIFNPTNLDADQWVKTLAEAGFGRVILTAKHHDGFCLWDSAYTKHDVASSPWKNGKGDV VKEVLEACAKYNIKFGVYLSPWDQNSEHYGDGNGGDYNEFYMNQLRELLTNYGPIAEVWM DGAKGSNVKQEYNFEEWFALIKKLQPECLIFSPQGPDIRWIGNEKGYAGEPCWSTIDIEK MKERENPTYLNNGEEGGPNWIVGESDVSIRPGWFYHESQDNEVKSLEKMMDIYFKSIGRN SVLLLNVPPNKEGKLHENDVNRLKKFGETIKELFNDDLALNKEVIVDGFANNDETYGANK IVDGDYDTYWAPDNSSKTGTIEIDLGGSKEFDVISLQEYIPLGQRVSSFNVEVLQGENWN KVYEGKTIGYKRLVRIAPTKGEKIRINITGSLEVPLINNVGVYKQPISIELPSGPPAGLK VLNDDNKGNELEQFNFSDGWIYETIHGEDDLGGDAHYTSKINATVNIKFKGTKFFLSGTK DSGHGIMEISIDGENPVEVDLYSPNRKSKEIVFESEDLSDGEHEVTVKCTGRKNSNSRGI VAHIDGAYVLDNGGKGMVEFEKVGYKVSENIGTATFKVIRKGGSNGKLEVNYDTLAGTAL NGVDYQTWSGTLAFNEGETEKTFDITIIDDKEKEEPKEFYLKLSDPIGGILGFNSRATVI INDDEQIKEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11095g
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
