Ta0085m (T. acidophilum )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9271d1BCt8p1
Keyword:
full-length cds
Species:
Thermoplasma acidophilum
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below. High number of silent and/or missense mutations in CDS. Reference sequence corrected.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
TaCD00369995
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1218nts
Open reading frame: 46 to 1263

TCAATATTTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAATTCTATTATCAAA
AACATAGAGATAATAGAGATGGGAGAAAAATCGCGTGAGACTTCCTCTCCCTGGAGTTCA
ACGATTTTGCTTGTGAAGATAACAAGCGCTGACGGACTTGTCGGATATGGCGAAGCACCG
ACGACACTCATGACGCTTCCGGTGCTTGAGAGCATGAAGGAAGTTGCAAGGGTCTTCAAC
GGAAAGGACTTCTTCAATGTGGAACGCAACGTTTTCGACTTTTACAGAAATTCGTTCTAC
CTTCCTGTGTCCATTGAGGCGACCTCAGCATTGAGCGCATTCGAAATAGCCTCATGGGAT
TTGATAGGAAAGCAGCTTGGTACTCCAGTGTACAAGCTTCTGGGCGGAGAAGTAAACCAG
AAGATGAGGGCCTATGCCAATGGCTGGTACAATGACTGCGTATCGCCAGATGACTTCCTC
AAGAAGGCAAAGGAAGTTGTGAAGATGGGATTCACAGCAATGAAGTTTGATCCCTTCGGT
GATAACTACGATTCAATAACAAACGACGGCCTAGAGAATGCCAGAAACATAGTTGGAACG
CTCAGATCCGAGCTTGGTGATAAGGTAGACCTGTTGATCGAATACCATGGCAGGTTTTCG
AAGATAGCAGCCATAAAGGCCGGCATGGCTCTGGAGGAATACCATCCGTTCTTTTACGAG
GAACCCCTGCATCCTGACCTTGATCACGACCTGCTAGATCTGAAGAACTACTACAGGGTT
CCGATAGCGCTTGGGGAAAGAGTACTTAACAAGAAGATATTTGCTAGGTTCATCTCACAG
AAACTGGTTGACATAATACAGCCAGATCTTACCAACTCAGGAGGCATACTGGAGGGAAAG
AAGATAGCCGCTATCGCAGATGGCTTCGGTATACCTGTAGCGCCGCACAACGCATTCGGG
CCCATACAGACCGCAGCAACGCTGAATCTGGATACTACCATAACCAACTTCATAATACAG
GAGAGCTTTGAAATGTTCTGGCCAGATTGGAAGAAGAAGCTCGTGTCCTCTGGCTATAAG
CTCGAGAATGGATACTTCACGCTGACCAACGAGCCTGGCGTATGCAAGGTTAACGAGGAC
ATCGTTGAAAAATATCAGGTTTCAGGTATGGAACCATTCGATGATAAGGAACCTCCATGG
GTTGTCTCTGGTACATTCAAGCCATACCGACAAAAAGAAGGGCATCATCACCATCACCAC
TGA

Protein sequence predicted based on nucleotide sequence

NSIIKNIEIIEMGEKSRETSSPWSSTILLVKITSADGLVGYGEAPTTLMTLPVLESMKEV
ARVFNGKDFFNVERNVFDFYRNSFYLPVSIEATSALSAFEIASWDLIGKQLGTPVYKLLG
GEVNQKMRAYANGWYNDCVSPDDFLKKAKEVVKMGFTAMKFDPFGDNYDSITNDGLENAR
NIVGTLRSELGDKVDLLIEYHGRFSKIAAIKAGMALEEYHPFFYEEPLHPDLDHDLLDLK
NYYRVPIALGERVLNKKIFARFISQKLVDIIQPDLTNSGGILEGKKIAAIADGFGIPVAP
HNAFGPIQTAATLNLDTTITNFIIQESFEMFWPDWKKKLVSSGYKLENGYFTLTNEPGVC
KVNEDIVEKYQVSGMEPFDDKEPPWVVSGTFKPYRQKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-9271d
Recommended expression in:
Not Applicable